简介：人的telomerase颠倒transcriptase(hTERT)在超过85%肿瘤房间被表示，但是通常没在正常房间被发现，它作为理想的肿瘤伙伴抗原(TAA)做hTERT开发没有在人的癌症免疫疗法损害正常纸巾，明确地破坏癌症的潜在的疫苗。这被考察hTERT或它的肽和早临床的trialsusing的immunogenicity上的研究的基本进展在最后十年的hTERT疫苗途径。

简介：Thereversetranscriptase(RT)proteinofhepatitisBvirus(HBV)hasbeensuccessfullyexpressedbyrecombinanttechnologyinEschericahiacoli(E.coli).Inthisstudyweaimedtodevelopasemi-quantitativeassayforthestudyofHBVRTproteinusingthissystem.CompleteHBVpolymerasegenefromawildtypevirus(rt306P)andthepolymerasegenefromamutant,withrt306Psubstitutedbyserine(rtP306S)wereseparatelyfusedtothemaltosebindingprotein(MBP)geneandexpressedinE.colirespectively.TheexpressionlevelsofHBVpolymerasegenesfromthewildtypevirusanditscounterpartmutantatrt306werecompared.Whentheseproteinsweresemi-quantifiedbyWesternblottingusingrabbitanti-TPserum,thertP306SmutantshoweddecreasedexpressionofMBP-HBVpolymerase.Bythismethod,wehaveshownthattheexpressionlevelofHBVRTcouldbeaffectedbysubstitutionsinitsaminoacidsequences,andthismethodcouldbeusedtostudythecharacteristicsofHBVRTprotein.

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简介：最近，韩国成人MMORPG“Reverseonline”在韩国玩家中非常盛行，这款游戏增加了不少成年玩家们喜爱的游戏要素，《Reverse》的空间背景是“地球”，但在这里登场的“地球”与我们所知道的地理完全不同，支配这个“地球”的是“ID”和“GURU”两个种族，虽然在外形上看起来跟我们所熟悉的人类相近，但他们向往的文明却是与我们所知完全不同的世界，

简介：Thetitlecompoundchlorpropham(CASnumber:101-21-3,C10H12ClNO2,Mr=213.66)waspreparedbytheadditionreactionof3-chlorophenylisocyanatewithisopropanol.Spectraldata,IR,NMRandMS,werereported.ThispaperprovidessomerelatedinformationaboutRegulatoryStatus,ToxicologicalEffects,EcologicalEffectsandEnvironmentalFatealso.

简介：AbstractAs human life expectancy continues to increase and the birth rate continues to decline, the phenomenon of aging is becoming more prominent worldwide. Therefore, addressing the problems associated with global aging has become a current research focus. The main manifestations of human aging are structural degeneration and functional decline of aging tissues and organs, quality of life decline, decreased ability to resist diseases, and high incidence rates of a variety of senile degenerative diseases. Thus far, no ideal treatments have been found. Stem cell (SC) therapies have broad application prospects in the field of regenerative medicine due to the inherent biological characteristics of SCs, such as their plasticity, self-renewal, and multidirectional differentiation potential. Thus, SCs could delay or even reverse aging. This manuscript reviews the causes of human aging, the biological characteristics of SCs, and research progress on age reversal.

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简介：Ameloblastomaisabenignbutlocallyaggressiveodontogenieneoplasmthataccountsfor10%ofalltumorsarisinginthemandibleandmaxilla(1).Eightypercentofameloblastomasariseinthemandible,andtheyareusuallyfoundinyoungadults.Itfrequentlyrecursifnotadequatelyresected.Therefore,thestandardtherapyforthistumoriscompleteboneresectionwithanadequatemarginofsafety:marginalorsegmentalosteotomy.However,aestheticdeformities,functionalimpairmentsandpsychologicalimpairmentsafterradicalsurgeryforlargeameloblastoma,havebeenseriousissues(1).

简介：Apotentialdualinhibitor(4)forexogenousabsorptionandendogenicsynthesisofcholesterolwasdesignedbasedontheconjugationoftheβ-lactampharmacophoreofezetimibeandtheδ-lactonepharmacophoreofstatins.Themergerofezetimibeandstatin4wassynthesizedfromp-hydroxybenzaldehydethroughaten-steproute.1HNMRanalysisshowedexistenceoffourpairsofenantiomers(5.7:5.7:1:1,molarratio).Andcompound4wasfoundtolowertotalglucose(TG)levelinratserumviaahigh-cholesterolandhigh-fatfeedingexperiment.

简介：Objective:ToinvestigatetheeffectsofE7080andN5-(1-iminoethyl)-L-ornithinedihydrochloride(L-NIO)oncolorectalcanceraloneandincombination.Methods:HT29colorectalcancercelllinefromSapInstitutewasused.Real-timecellanalysis(xCELLigencesystem)wasperformedtodeterminetheeffectsofE7080andL-NIOoncolorectalcellproliferation.WhileapoptosiswasdeterminedwithAnnexinVstaining,andtheeffectofagentsonangiogenesiswasdeterminedwithchorioallantoicmembrane(CAM)model.Results:WefoundthatE7080hasastrongantiproliferativeeffectwithanhalfmaximuminhibitionofconcentration(IC50)valueof5.60×10–8mol/L.AlsoithasbeenobservedthatE7080showedantiangiogenicandapoptoticeffectsonHT29colorectalcancercells.AntiangiogenicscoresofE7080were1.2,1.0and0.6for100,10and1nmol/LE7080concentrations,respectively.Furthermore,apoptosishasbeendetectedin71%ofHT29colorectalcancercellsafteradministrationof100nmol/LE7080whichmayindicatestrongapoptoticeffect.MeanwhileadministrationofL-NIOalonedidnotshowanyeffect,butthecombinationofE7080withL-NIOincreasedtheantiproliferative,antiangiogenicandapoptoticeffectsofE7080.Conclusions:ResultsofthisstudyindicatethatE7080maybeagoodchoiceintreatmentofcolorectaltumors.FurthermoretheincreasedeffectsofE7080whencombinedwithL-NIOraisethepossibilitytousealowerdoseofE7080andthereforeavoid/minimizethesideeffectsobservedwithE7080.

简介：AbstractReverse genetics via targeted modification of gene sequences to obtain a phenotype and the inference of a gene's function or regulatory mechanism is widely used as a potent tool in viral biology and application. However, while reverse genetics has contributed significantly to our understanding of molecular biology and the pathogenesis of viruses, its accessibility (operation) and openness (data) have raised many concerns regarding biosafety and biosecurity. In this review, we retrospectively examine the development of reverse genetics and its applications in virology, then emphasize global biosafety and biosecurity concerns regarding reverse genetics, and summarize global regulations, governance, and laws on reverse genetics. This review seeks to enhance our understanding and rational application of reverse genetics technology for the benefit of humankind.

简介：有弹性的移植被采用multicomponent处理地震数据的向量广泛地付了注意。光线基于的有弹性的Kirchhoff移植有象高灵活性和高效率的如此的性质。然而，它没能解决multipath引起的许多问题。在另一方面，有弹性的反向时间的移植(请读使用手册)基于双向波浪方程被知道能够处理这些问题，但是当在3D情况和速度模型大楼中适用时，它是极其昂贵的。基于有弹性的Kirchhoff-Helmholtz积分，我们计算decoupled由介绍有弹性的格林的向后继续的wavefields为P-waves,和S波浪工作，它被elastodynamicGaussian横梁的求和表示。PP和改正极性的PS图象被计算关联在之间获得向下并且decoupled向后继续的向量wavefields，在极性修正被分析在极化之间的关系执行的地方，变换PS的方向飘动并且接口上的事件角度。到大程度，我们的方法把基于光线的移植的高效率与波浪方程的高精确性相结合基于的反向时间的移植。到从差错模型和Marmousi2的合成数据集建模的multicomponent的这个方法的申请表明新方法的有效性，灵活性和精确性。

简介：极其细小的chitosan粒子被由水，三重氢核X-100，octanol和环己烷组成的反向的微乳液准备。粒子被采用并且比较了使用TEM和红外的准备极其细小的chitosan的二个方法，和为极其细小的chitosan粒子的形成的可能的机制被建议。试验性的结果证明联合了离子的冻结和cross-linking的方法与92nm的一条平均直径给了一致地大小的chitosannanoparticles，当没有离子的冻结的cross-linking与188nm的943nm和宽度的平均长度生产了细长纤弱的chitosan粒子时。

简介：近年来,我国房地产市场发展迅速,人们住房条件得到普遍改善。随着私人购房的增加和人口的老龄化,社会上存在另外一种现象:一些城市里的老人通过拆迁或其他方式持有价值不菲的房产,由于自身需要居住而无法出租,然而老人们的收入又都较低,很多都无法满足日常生活的开支,需要儿女们的资助。这就出现了老人们拥有大量的财富,但有可能日子却过得比较窘迫的奇怪现象。如何提高老人们的生活水平一直是各国政府头疼的一个话更多还原

简介：Anoptimumdesignapproachtoreverse-flowcyclonesbasedontheconceptofoptimizingcyclonedimensionsisintroducedinthispaper.Thisapproachinvolvesoptimizingcyclonedimensionsbycategories,calculatingcycloneperformancebycorrelatingsimilitudenumbersandoptimizingthecombinationoffourcycloneparameters,D1KA1dr^-andvi,whichhasbeenproventobeapplicablenotonlyforsingle-stagecyclone,butalsoformultistagecycloneseparators.ApplicationsofthedesignedcyclonesinFCCunitsandacrylonitrilereactorsarealsopresented.

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简介：AbstractLow-level viremia (LLV) was defined as persistent or intermittent episodes of detectable hepatitis B virus (HBV) DNA (<2000 IU/mL, detection limit of 10 IU/mL) after 48 weeks of antiviral treatment. Effective antiviral therapies for chronic hepatitis B (CHB) patients, such as entecavir (ETV), tenofovir disoproxil fumarate (TDF), and tenofovir alafenamide (TAF), have been shown to inhibit the replication of HBV DNA and prevent liver-related complications. However, even with long-term antiviral therapy, there are still a number of patients with persistent or intermittent LLV. At present, the research on LLV to address whether adversely affect the clinical outcome is limited, and the follow-up treatment for these patients is open to question. At the same time, the mechanism of LLV is not clear. In this review, we summarize the incidence of LLV, the association between LLV and long-term outcomes, possible mechanisms, and management strategies in these patient populations.

简介：Inrecentyears,intravascularultrasound(IVUS)follow-upisalwaysusedintheevaluationofthedevelopmentofatherosclerosis,anditcanalsobeusedastheendpointofdrugtherapyinclinicalobservation.Since1994,thefirststatinlipid-lowering4Sexperimentresultswasreported,thefollowingstatinforlipid-loweringtestsrepresentedbyREVERSALPROVE-IT,TNT,IDEAL,ASTEROIDandJUPITERstronglyconfirmedthatfurtherreducetheefficacyoflow-densitylipoproteincholesterol(LDL-C)(toenhancethelipid-loweringtreatment)accesstoincreaseeffectofthecardiovascularprotectionandalsoreversetheplaques'progress.Butscholars'opinionsonthemeritsanddemeritsofenhancestatincholesterol-loweringtherapyhasbeenindebate.Wereviewtherecentworkonstatinsandreversalofarterialplaquesforanumberofclinicalstudies.