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简介：InvitrodetectionmethodforthesensitivityofMagnaporthegriseatotricyclazolewasstudied,andthepotentialresistanceriskofblastdiseasetotricyclazolewasassessed.BothEC50ofhyphalmelanization(EC50-H)andminimuminhibitiveconcentrationofmelanizationinappressorial(MIC-A)byinhibitortricyclazoleshowedpositivecorrelationtotheEC50oftricyclazoleagainstblastdiseasetestedinvivo,withrelativeco-efficiency(R2)of0.8995and0.8244,respectively.However,stabilityandreproducibilityofEC50-HwerebetterthanthoseofMIC-A,suggestingthatitcouldbeusedtodetectthesensitivityofM.griseatotricyclazoleinvitro.TricyclazolesensitivityoftheprogeniesderivedfromsinglesporesofthemostsensitiveisolateDY2andtheleastsensitiveisolateGY6detectedinsensitivitymonitoringin2000wasnotstable,withmeanEC50valuesof4.4968μg/mLand5.4010μg/mL,respectively,indicatingthatthedifferenceinEC50betweenDY2andGY6wasnotcausedprobablybyresistancevariation.EC50ofGY6didnotincreasesignificantlywhencontinuouslyselectedfortwentygenerationsundertheselectionpressureoftricyclazoleinvivo.However,thesensitivityofDY2wasdecreasedby10-foldafterselectedfortwentygenerations.TheresultssuggestedthattricyclazolewasstilllowresistanceriskforM.griseainChina.

简介：Genotypingbysequencing(GBS)istherecentapproachofnext-generationsequencingtechniquefordiscoveringandgenotypingsinglenucleotidepolymorphisms(SNPs)incropspecies.Genotypicvariationstudies(SNPsandinsertion-deletions/InDels)wereperformedusingfourricelinesbasedonGBSdatabyaligningtothereferencegenomeNipponbare.LocalaromaticricelandraceTulaipanjiwascrossedwithRanjit,andtwodistinctlineswereidentifiedfromtheprogenies:onelinewithawnsandaromatraitsandtheotherwithoutawnsandaroma.TotalnumberofSNPsandInDelsidentifiedwere52810and4327atreaddepth10,respectively.OutofthetotalpolymorphicSNPs/InDels,16490wereintergeneric,7812wereinsidegene,and4435wereintronic.Phylogenetically,Tulaipanjiwasclosertothereferencegenomenipponbare.Basedonrecurrentparentgenomeanalysis,outof10013alleles,92.52%wasintrogressedintoprogeny-awnfromTulaipanjiand7.48%fromRanjit,whereasprogeny-awnlesscarried89.19%allelesfromRanjitandonly10.81%allelesfromTulaipanji.Inaddition,progeny-awnwasthehighestheterozygous(83.88%)andprogeny-awnlesswastheleast(2.24%)atthisfifthgenerationofrecombinantinbredlines.TheseSNPvariationsmaybelinkedtothephenotypictraitsandcanbeutilizedincropimprovementthroughlinkagemapping.TheseresultssuggestthataddingahighdensityofSNPmarkerstoamappingorbreedingpopulationthroughGBShasagreatvaluefornumerousapplicationsinricebreedingandgeneticsresearch.

简介：为米饭核心收集的十一评估参数被估计基于ongenotypic价值和分子的标记信息。与混合线性模型相结合的蒙特卡罗模拟被用来从环境消除干扰以便拉更可靠的结果。范围(CR)的巧合率是最佳的参数。吝啬的Simpson索引(M_D)，基因差异(M_I)的吝啬的Shannon编织索引和吝啬的多型性信息内容(M_(照片))是重要的评估参数。变异系数(VR)的可变的率能为评估核心收集的变化度充当一个重要引用参数。多态的loci(p)的百分比能为核心收集的尺寸被用作一个决心参数。吝啬的差别百分比(MD)是为核心收集的可靠性判断的一个决心参数。为在研究选择的核心收集的有效评估参数能在不同plantgermplasm人口为采样百分比被用作标准。

简介：Thesystemofriceintensification(SRI)isaproductionsystemthatinvolvestheadoptionofcertainchangesinmanagementpracticesforricecultivationthatcreateabettergrowingenvironmentforthecrop.Thissystemwascomparedwithconventionalpracticesandassessedunderorganicandinorganicmanagement.SRIpracticesshowedsignificantresponseinrootnumber,numberofeffectivetillersperhill,daystofloweringandharvestindex.Inaddition,SRIwasfoundeffectiveinminimizingpestanddiseasein...

简介：ThaijasminericeKDML105isconsumedaroundtheworld.BKOS,PKOSandTKOSarenewcultivarsproducedfromlow-energyionbeaminductioninKDML105.ThepurposeofthisstudyistocomparethemorphologyandanatomybetweenKDML105andthethreenewcultivars.Seedsofthefourcultivarsweregerminatedandgrowninpotsuntilfloweringphase.Theplants'organswereobservedandthelengthsofculms,ligules,leavesandpaniclesweremeasured.Leafsurfaceareawascalculatedandnumbersofroots,spikeletsandtillerswerecounted.BKOSandPKOShadsignificantlyshorterculmsthanKDML105andTKOS.ThelargestleafareawasfoundinKDML105followedbyTKOS,BKOSandPKOS,respectively.NumbersofrootsandtillersinBKOSandTKOSweresignificantlyfewerthanthoseinKDML105andPKOS.ThenumberofspikeletsperplantinBKOSwasthelowestamongallcultivars.Foranatomicalcomparison,crosssectionsofculmsandrootswereobserved.Allplantshadasimilararrangementoftissues,butthenumberandsizeofcellsweredifferent.Furthermore,longitudinalsectionsofculmsshowedthatthelengthsofepidermalandparenchymacellsweredirectlyrelatedwiththelengthoftheculm.Tocomparetheleaves,bothstomataandepidermalcellswerecountedandthelengthsoftheguardcellsweremeasured.ThelengthsofguardcellsofBKOSandPKOSwereshorter,butthestomataldensityandthestomatalindexweresignificantlygreaterthanthoseofKDML105.ForTKOS,thoughthelengthofguardcellswasshorterthanthatinKDML105,thedifferencewasnotsignificant.However,thestomataldensityandstomatalindexweresignificantlyhigherthanthoseinKDML105.