简介：ChinesepopulationsinfectedwithHIV-1.Methods:GenomeDNAfromperipheralbloodmononuclearcells(PBMCs)of78HIV-1infectorswasamplifiedbypolymerasechainreaction(PCR).CCR5,CCR2bandSDF1genefragmentswereobtainedfromrestrictivefragmentlengthpolymorphism(RFLP)and/orCCR△32,CCR5m303,CCR2b-64IandSDF1-3'Aallelicgenes'mutationalfrequenciesweresequenceddirectlyfromPCRproducts.Results:NoneofCCR5△32,CCR5m303genemutationwerefoundin78subjectswithHIV-1infection.TheallelicgenemutationfrequenciesofCCR2b-64IandSDF1-3'Acorrespondingto14.9-34.0%and17.6-38.2%of95%CI,were22.79%and26.92%respectively.TheircolonydistributionconformedtotheHardy-Weinbergequilibrium.Conclusion:TheHIV-1infectionsfoundatpresentareallsusceptiblepopulationofCCR5△32andCCR5m303.ThepolymorphismandfrequenciesofCCR5△32,CCR5m303,CCR2b-64IandSDF1-3'AallelesfromChineseHIV-1infectedpopulationweredisclosedinthisstudyforthefirsttime,whichisofsignificanceforstudyingthegeneticresistancetosusceptibilitytoHIV-1infectionaswellasAIDSdiseaseprogression.

简介：ToinvestigatethephenotypicknockoutofHIV-1chemokinecoreceptorCXCR4andCCR5byintrakinesanditsinhibitoryeffectonHIV-1infection.PrimaryhumanPBLsweretransducedwiththerecombinantvectorpLNCX-R-K-S-K(△NGFR),followedbyanti-NGFR/anti-IgG-magneticbeadmethodselectionandFCMdetection.ThetransducedPBLswereinfectedwithDP1HIV-1virusthereafterenvelope-mediatedsyncytiumformationandp24detectionwerecarriedouttostudytheblockageofHIV-1infectionbyco-inactivationofCCR5andCXCR4.pLNCX-R-K-S-K(△NGFR)-transducedPBILswereisolatedwithananti-NGFR/anti-IgG-magneticbeadmethod.Afterisolation,about70%ofthePBLswerepositivefortheNGFRmarker.WhenthetransducedPBLswereinfectedwithDP1HIV-1virus,envelop-mediatedsyncytiumformationwasalmostcompletelyinhibitedbypLNCX-R-K-S-K(△NGFR)transfection.Also,p24antigenwasverylowintheculturesofpLNCX-R-K-S-K(△NGFR)transducedPBLs.pLNCX-R-K-S-K(△NGFR)transductioninhibitedtheproductionofDP1p24antigenby15%,43%and19%ondays4,7and10respectively.ThelymphocyteswiththephenotypicknockoutofCCR5andCXCR4couldprotectprimaryhumanPBLsfromDP1HIV-1virusinfection.

简介：WeareracingwithHIV-1,theetiologicagentforAIDSinhumanbeings[1,2],withtwopossibleendconsequences:ifwewin,HIV-1willbeunderourcontrolbyimmunologicortherapeuticmeasures;ifHIV-1wins,theSIVAfricanmonkeys'storywouldrepeatinhumans,i.e.,onlythefewindividualsthatarenotkilledbythevirus

简介：ToexplorethemechanismoftheinhibitionofHIV-1byMycoplasmafermerttans,culturesupernatantsandthallodicproteinsfromM.fermerttansPG18werepreparedandtheproteincomponentsofthesupernatantswerepurifiedwithhighperformanceliquidchromatography(HPLC).Theinhibitoryactivitiestoreversetranscriptase(RT)andthenucleaseactivitiesweredetected;theinfluenceofM.fermerttansonIL-10secretionbybothnormalandH1V-1infectedhumanPBMCweredetermined,andtheinhibitoryeffectofrhIL-10onH1V-1replicationwasdetectedwithEI,ISAmethod.Theresultsshowedthatthepurifiedproteinswithamolecularweightof67-100kDaor10-25kDashoweda36%or34%inhibitoryac-tivitytoRTandpartialnucleaseactivity.ThethallodicproteincouldinducebothnormalandH1V-1infectedPBMCtosecretIL-10remarkably,andtothelatter,thiseffectwasmoreapparent.WhilerhIL-10couldinhibitreplicationofH1V-1inPB-MCinvitroinadose-dependantmanner.ItconcludesthattheinhibitoryeffectoftheM.fermentansPG18culturesupernatantsonRTandthepromotingeffectofPG18thallodicproteinonIL-10secretioninPBMCexplainthemechanismsofinhibitiontoHIV-1byM.fermentansPG18.

简介：Objective:Toanalyzesubtypesandquasi-speciesofisolatedvirusesfromHIV-1infectedindividualsamongthepopulationepidemiologicaldynamicsoflocalHIV-1isolates,thuslayingafoundationfordesigningacandidateAIDSvaccine.Methods:Byhetero-duplexmobilityassay(HMA)andsinglestrandconformationpoly-morphism(SSCP)analysisonampliconsfromsingle-primedpolymerasechainreaction(SP-PCR),subtypesandquasi-speciesoftestedHIV-1isolateswereelucidated,andampliconsweresequencedforconfirmation.Results:Specificampliconsfromdifferentsubtypesandquasi-speciesofHIV-1couldbediscerniblebyHMAandSSCPanalysis.HIV-1isolatesfromdifferentpatientsmightbeeitheradifferentsrbtypeoranidenticalsubtype,andHIV-1isolatesfromanindividualwerepresentinapopulationofquasi-species.

简介：Objective:ToevaluatethehumoralimmuneinductioninratsofacandidateAIDSvaccineexpressingthegagp24genefromasubtypeBHIV-1isolate.Methods:Theamplifiedp24genewasinsertedintoaneukaryoticexpressionvectortoformthesupercoiledDNAvaccine.ThelinearizedexpressedDNAvaccinewaspreparedfromtheexpressionplasmidbypolymerasechainreaction(PCR).TheantigengeneexpressioninratsofthelinearizedandsupercoiledDNAvaccineswereinvitroandinvivodetected.Results:InvitrotranscriptionandNorthernhybridizationshowedthatthelinearizedDNAvaccinecouldsynthesizeamountsofp24mRNAsimilartothesupercoiledDNAvaccine.AntibodyassaysofinoculatedratsconfirmedthatthelinearizedexpressionDNAcouldinduceaslightlyhigherantibodytiterthantheexpressionplasmid,whilethehighestantibodytiterhadbeeninducedbyplasmidplusadjuvantinoculation.Conclusion:TheconstructionofacandidateAIDSvaccinebasedonthep24genecouldshedlightonapotentialHIVvaccine,meritingevaluationinarhesusmacaqueSHIV-AIDSmodel.

简介：Objective:Toobservetheeffectivenessofhighlyactiveantiretrovirustherapy(HAART)onHIV/AIDSpatients.Methods:UsingHIV-Iquantitativemethodsandimmunologicalfunctioninspection,wemonitored4HIV/AIDSpatientswhoweresufferingfromimmunologicaldeficiencyandweretreatedwithHAART.Results:ThereproductionofHIVinall4patientswasefficientlycontrolledatthe4thweekofthetreatment.Theaverageviralloaddecreasedby1.99Log/ml(0.73-2.46Log/ml).ThenumberofCO+4andCD+sshowedasteadycontinuousincrease4to12weeksafterthetreatment,withanincreaseof67.2%and103.0%respectively.CorrelativestudyamongdifferentvariablesafterthetreatmentrevealedthatpositivecorrelationexistedbetweenthenumberofCD+4andCD+3aswellasCD+8,whilenegativecorrelationexistedbetweenthenumberofCD+4andplasmaviralload.Conclusion:HIV-Iquantitativemethod(plasmaviralload)andthenumberofCD+4inperipheralbloodcanbeusedasimportantreferenceindicatorsinevaluatingHAART.

简介：LTR位于HIV前病毒同DNA基因组的两末端,Gag基因位于5′端LTR的下游。LTR对转录的调控作用主要是由5′端LTR进行的。LTR具有启动子的作用,在结构上具有真核启动子的典型特征。另外,HIV-1Gag蛋白还有能够自我装配成病毒样粒子(viruslikeparticle,VLP)并从细胞中释放出来的重要特性。我们利用痘苗病毒表达系统,探索了在细胞质环境中LTR中的多种功能结构对Gag蛋

简介：Objective:Toamplifyantigengenesfrompatientswithhumanimmunodeficiencyvirustype1(HIV-1)inGuangdongProvinceforcandidateAIDSvaccinedesign.Methods:Viralnucleicacidwasisolatedfrom10HIV-1infectedindividuals'peripheralbloodcollectedduring1995-2000inGuangdongProvince.Theviralgagp24geneandenvgp120genewereamplifiedbynested-PCRandsequenced.ThehomologiesamongHIV-1isolateswerecomparedwithHIV-BLAST.Results:Among10HIV-1isolates,ninearehomologoustovirusesofsubtypeB,andoneishomologoustovirusesofsubtypeE.Conclusion:SubtypeBvirusesofHIV-1arepredominantlypresentinGuangdongProvince.

简介：ThisarticleisageneralreviewoftheevolvementofHIV/AIDS-relatedpublicpoliciesinChinasince1980's.Ittrackstheimportantlaws,regulationsandothergovernmentaldocumentsinregardtoHIV/AIDSpreventionmainlyatcentrallevel.

简介：美国马利兰州巴尔的摩的约翰．霍普金斯大学学者ThomasQuinn及其同事报告说，血清中有较高浓度HlV一1的个体较那些有较低浓度的个体更易使其配偶感染。HIV-1RNA浓度上升10倍者较上升2倍者更易造成传播。Quinn指出，“结果提示静脉血中较低的病毒负荷可以降低其传播力。”

简介：Activehost-pathogeninteractionstakeplaceduringinfectionofhumanimmunodeficiencyvirustype1(HIV-1).Outcomesoftheseinteractionsdeterminetheefficiencyofviralinfectionandsubsequentdiseaseprogression.HIV-infectedcellsrespondtoviralinvasionwithvariousdefensivestrategiessuchasinnate,cellularandhumoralimmuneantiviralmechanisms.Ontheotherhand,thevirushasalsodevelopedvariousoffensivetacticstosuppressthesehostcellularresponses.Amongmanyoftheviraloffensivestrategies,HIV-1viralauxiliaryproteins(Tat,Rev,Nef,Vif,VprandVpu)playimportantrolesinthehost-pathogeninteractionandthushavesignificantimpactsontheoutcomeofHIVinfection.OneofthebestexamplesistheinteractionofVifwithahostcytidinedeaminaseAPOBEC3G.AlthoughspecificrolesofotherauxiliaryproteinsarenotaswelldescribedasVif-APOBEC3Ginteraction,itisthegoalofthisbriefreviewtosummarizesomeofthepreliminaryfindingswiththehopetostimulatefurtherdiscussionandinvestigationinthisexhilaratingareaofresearch.

简介：目的比较人的血液和尿液样本中HIV-1抗体的一致性.方法在广东省两所劳动教养所收集346名HIV高危者的血液和尿液样本,分别用血液和尿液ELISA初筛试剂检测HIV-1抗体.结果346份血液和尿液样本各18份阳性,其中有17人的血液和尿液平行样本均为HIV-1抗体阳性,血液和尿液样本HIV-1抗体检测的一致性为99.4%.结论在采集血液样本不便的情况下,可利用尿液初筛诊断试剂进行HIV感染的筛查和监测.

简介：HIVinfectionandAIDShasemergedasamajorpublichealthproblemallovertheworld.Inthe1980s,theinfectionwasfirstfoundtobetransmittedthroughhomosexualactivityandbloodproducttransfusion.Nowitisspreadingamongheterosexualsandinjectiondrugusers,andcanbetransmittedfrommotherstoinfants.

简介：Objective-TocomparetheconsistencyoftheresultsfromdetectingHIV-1antibodyinthepairedurineandserumspecimensfromdrugusersbyELISA.Methods:Thepairedurineandserumspecimensfrom273drugusersdetainedatadetoxificationunitwerecollected,andtheHIV-1antibodiesinthespecimensofthemwerescreenedbyurineandserumELISAkits,respectively.Results:Of273serumspecimens,94onesshowedpositivereactionandamong94counterparturinespecimens,93onesalsoappearedpositivereaction.Takingtheresultstogether,theconsistentrateofHIV-1antibodyscreenedbyurineandserumELISAkitswas99.6%.Conclusion:TheurineELISAkit,whichscreenedHIV-1antibodyofurineshowingalmostthesameresultstestedbyserumELISAkit,isreliable.ItisproposedthaturineELISAbeintroducedinmanyfields.

简介：HIV-1粒子中含有3类结构蛋白,即核心蛋白(Gag)、聚合酶(pol)和外膜蛋白(Env)。Gag蛋白既具有诱导体液免疫和细胞免疫的抗原决定簇,又能够自我装配成病毒样粒子

简介：Theimmuneefficiencyofarecombinantadenovirustype5withtype35fibercontainingHIV-1gaggene(rAd5/F35-mod.gag)wasinvestigatedinBALB/cmice,inwhichtherAd5/F35-mod.gagwasfirstlyidentifiedwithPCR,thentransfectedto293cellsandtheinvitroexpressionlevelofGagproteinwasdeterminedbyWesternblottingandindirectimmuno-fluorescentassay.MicewereimmunizedwithintramuscularinjectionsofrAd5/F35-mod.gag,rAd5-mod.gagorDNAandwereboostedafter3weeks.Totesttheeffectofpre-existinganti-viralimmunityonimmunization,micewerealsoinjectedwithAd5-GFPvectorandthenimmunized4and7weekslaterwithAd5/F35-mod.gagvector.TheP24-specificIgGantibodyinseraofimmunizedmicewasdeterminedbyELISAandthespecificcytotoxicTlymphocyte(CTL)responsewasassayedbyintracellularcytokinestaining.ItwasdemonstratedthattherAd5/F35-mod.gagvectorcouldexpressefficientlytheHIVGagproteinin293cellsinvitroandinducestrongHIV-specificimmuneresponsesinvivo.ThestrongestCTLandserumIgGresponseoccurredwhenmicewereimmunizedtwicewithinjectionofrAd5/F35alone,buttheanti-Ad5antibodyafterprimaryinfectionwithadenoviruscouldinhibitthespecificimmuneresponsesinducedbyrAd5/F35vector.ItisconcludedthatsingleimmunizationwithrecombinantadenovirusrAd5/F35-mod.gagcaninducespecificCTLandserumIgGantibodyresponsesinmice,buttheimmunogenicityofrAd5/F35iscomparablyweakerthanthatofrAd5.

简介：摘要目的了解流行于柳州市吸毒者艾滋病病毒HIV-1毒株的亚型及各种亚型的分布特点。方法对34例HIV感染者进行详细的流行病学调查，并采集外周静脉防凝血,提取前病毒DNA进行体外扩增，获得包膜蛋白(env)、核心蛋白(gag)、tat区基因的核酸片段，并对各基因区核苷酸进行测定和分析。结果发现在所有目标人群中共存在B、C2种亚型以及CRF07-BC（44.12%）和CRF01-AE(55.88%)2种重组毒株。结论柳州市HIV-1亚型以B、C为主，重组毒株以CRF07-BC和CRF01-AE几乎各占一半，柳州市HIV-1亚型比较单一，控制柳州市HIV-1亚型的多样性，做好防艾任重而道远。

简介：目的了解在广东省受血及献血者中发现的HIV-1亚型的流行规律及其与国际参考株的同源性.方法应用套式聚合酶链反应(PCR)对14例采自于广东省HIV-1抗体阳性的受血者或献血者淋巴细胞富集液的核酸样品进行扩增,并使用AB1377型测序仪对扩增产物测序后,对其ENV基因C2-V3段的核酸序列进行比较分析.结果14份血样中,7份为泰国B'亚型,与国际参考株RL42的距离最近,基因离散率为(6.082±2.607)%,组内离散率为(5.963±2.383)%;4份为AE重组亚型,与国际参考株TH.90.CM240最近,基因离散率为(8.900±1.830)%,组内离散率为(13.810±1.317)%;2份为07-BC重组亚型,与国际参考株CN.97C54A最近,基因离散率为(4.155±1.223)%,组内基因距离为6.36%;1份为08-BC重组亚型,与国际参考株97CNGX-9F最近,基因距离为0.97%.结论本次检测的广东省受血及献血者HIV-1以泰国B'亚型为主,也存在主要在性途径感染人群中流行的AE重组亚型和吸毒者中流行的07-BC、08-BC重组亚型.

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