Antigen Gene Cloning and Expression of HIV-1 Toward AIDS Vaccine Design—Ⅱ.Shbtype Classification and Quasi-species Identification of HIV-1-中国期刊网

摘要 Objective:Toanalyzesubtypesandquasi-speciesofisolatedvirusesfromHIV-1infectedindividualsamongthepopulationepidemiologicaldynamicsoflocalHIV-1isolates,thuslayingafoundationfordesigningacandidateAIDSvaccine.Methods:Byhetero-duplexmobilityassay(HMA)andsinglestrandconformationpoly-morphism(SSCP)analysisonampliconsfromsingle-primedpolymerasechainreaction(SP-PCR),subtypesandquasi-speciesoftestedHIV-1isolateswereelucidated,andampliconsweresequencedforconfirmation.Results:Specificampliconsfromdifferentsubtypesandquasi-speciesofHIV-1couldbediscerniblebyHMAandSSCPanalysis.HIV-1isolatesfromdifferentpatientsmightbeeitheradifferentsrbtypeoranidenticalsubtype,andHIV-1isolatesfromanindividualwerepresentinapopulationofquasi-species.

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Antigen Gene Cloning and Expression of HIV-1 Toward AIDS Vaccine Design—Ⅱ.Shbtype Classification and Quasi-species Identification of HIV-1

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Antigen Gene Cloning and Expression of HIV-1 Toward AIDS Vaccine Design—Ⅱ.Shbtype Classification and Quasi-species Identification of HIV-1

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