简介:分子标记辅助聚合育种是累加有利基因的有效手段.培育粳型亲籼系是有效克服水稻籼粳亚种间杂种不育性,从而利用水稻亚种间杂种优势的重要途径之一.本研究利用以PCR为基础的分子标记进行辅助选择,对不同粳型亲籼系中不同分化度的特异亲和基因进行了聚合,并将4个抗白叶枯病基因和来源于IR24的两个恢复基因导入粳型亲籼系中.主要结果如下:1、以粳型亲籼系G2417-2-1和粳型广亲和系G2605为亲本构建分离群体,利用本研究筛选的与S-b,S-c,S-d三个F1花粉不育基因座位紧密连锁的PCR标记进行辅助选择.在F2共选择到特异亲籼聚合植株6株,它们分别是58号,93号,94号,115号,139号,200号;广亲和聚合植株4株,它们分别是11号,14号,121号,177号.2、对当选聚合系的亲籼性和亲粳性综合分析表明:各个特异亲籼聚合系的亲粳性之间及各个广亲和聚合系的亲籼性之间都有显著差异.特异亲籼聚合系的平均亲籼性和平均亲粳性与亲本G2417-2-1相比都没有显著差异.广亲和聚合系的平均亲籼性高于亲本G2605,平均亲粳性显著低于亲本G2605.这些聚合系的亲和性与其MAS基因型相一致.3、利用四类粳型亲籼系与携带有2个恢复基因和4个抗白叶枯病基因的品系构建回交群体,应用本研究筛选的以PCR为基础的分子标记进行辅助选择.在BC1F1共选择到2个恢复基因和4个抗白叶枯病基因全杂合的植株19个,其中以IC31为受体的5株,以IC32为受体的11株,以IC33为受体的2株,以IC34为受体的1株.4、当选的19个单株自交繁殖BC1F2,利用与目标基因紧密连锁的单一分子标记进行MAS.共选择到各类可供进一步利用的材料393株,其中携带有两个纯合恢复基因的植株158株,同时携带有两个纯合恢复基因和两个纯合显性抗白叶枯病基因的植株40株.5、从上述158个植株中选出两个显性抗性基因均纯合或者任意三个抗性基因均纯合的�
简介:本研究利用Wx基因微卫星特异性分子标记"484/485"对13份国内常用保持系进行PCR检测,筛选出具有中等AC含量、Ⅰ型带型的优质保持系"宜香1B"作为优质供体。配制"协青早B/宜香1B",对其F1、F2代单株用"484/485"PCR分子检测,证明其带型符合一对基因分离模式。在此基础上,对"协青早B/宜香1B"的杂交、回交和自交的低世代群体(BC1F1、BC1F2)进行"484/485"分子标记辅助选择,再结合优质不育系其它性状选择,快速育成了中等AC含量、垩白率低、透明度高、不育特性和农艺性状稳定的籼型优质三系不育系"浙农3A",并于2009年9月顺利通过了浙江省品种审定委员会组织的专家现场鉴定。本文还就利用"484/485"分子标记有效改良稻米AC含量等问题进行了讨论。
简介:CMSphysicistsneedtoseamlesslyaccesstheirexperimentaldataandresults,independentoflocationandstoragemedium,inordertofocusontheexplorationforthenewphysicssignalsartherthanthecomplexitiesofworldwidedatamanagement.Inordertoachievethisgoal,CMShasadoptedatieredworldwidecomputingmodelwhichwillincorporateemergingGridtechnology.CMShasstartedtouseGridtoolsfordataprocessing,replicationandmigration,ImportantGridcomponentsareexpectedtobedeliveredbytheDataGridprojects.likeprojects,CMShascreatedasetoflong-termrequirementstotheGridprojects.Theserequirementsarepresentedanddiscussed.
简介:TheCMSexperimentattheCERNLHCcolliderisproducinglargeamountsofsimulateddatainordertoprovideanadequatestatisticfortheTriggerSystemdesign.Theseproductionsareperformedinadistributedenvironment,prototypingthehierarchicalmodelofLHCcomputingcentersdevelopedbyMONARC.AGRIDapproachisbeingusedforinterconnectingtheRegionalCenters.Themainissueswhicharecurrentlyaddressedare:automaticsubmissionofdataproductionrequeststoavailableproductioinsites,datatransferamongproductionsites,“best-replica”locationandsubmissionofenduseranalysisjobtotheappropriateRegionalCenter,Ineachproductionsitedifferenthardwareconfigurationsarebeingtestedandexploited.Furthermorerobustjobsubmissionsystems.whicharealsoabletoprovidetheneededbookkeepingoftheproduceddataarebeingdeveloped.BOSS(BatchObjectSubmissionSystem)isaninterfacetothelocalcomputingcenterschedulingsystemthathasbeendevelopedinordertoallowrecordinginarelationaldatabaseofinformationproducedbythejoberunningonthebatchfacilitiesAsummaryofthecurrentactivitesandaplanfortheuseofDataGridPM9toolsarepresented.
简介:TheCMSOOreconstructionprogram-ORCA-hasbeenusedsince1999toproducelargesamplesofreconstructedMonte-Carloeventsfordetectoroptimization,triggerandphysicsstudies,TheeventsarestoredinseveralObjectivityfederationsatCERN,intheUS,Italyandothercountries.ToperformtheirstudiesphysicistsusedifferenteventsamplesrangingfromcompletedatasetsofTBytesizetoonlyafeweventsoutofthesedatasets.WedescribetheimplementationoftheserequirementsintheORCAsoftwareandthewaycollctionsofeventsareaccessedforreading,writingorcopying.
简介:OfflineSoftwaresuchasSimulation,Reconstruction,Analysis,andVisualisationareallinneedofadetectordescription.Theseapplicationshaveseveralcommonbutalsomanyspecificrequirementsforthedetectordescriptioninordertobuilduptheirinternalrepresentations.Toachievethisinaconsistentandcoherentmanneracommonsourceofinformation,thedetectordescriptiondatabase,willbeconsultedbyeachoftheapplications.TheroleandsuitabilityofXMLinthedesignofthedetectordescriptiondatabaseinthescopeoftheCMSdetectorattheLHCisdiscussed.DifferentaspectssuchasdatamodellingcapabilitiesofXML,toolsupport,integrationtoC++representationsofdatamodelsaretreatedandrecentresultsofprototypeimplementationsarepresented.
简介:Powerfulmainstreamcomputingequipmentandtheadventofaffordablemulti-Gigabitcommunicationtechnologyallowustotackledataacquisitionproblemswithclustersofinexpensivecomputers.Suchnetworkstypicallyincorporateheterogeneousplatforms,real-timepartitionsandcustomdevices.Therefore,onemuststriveforasoftwareinfrastructurethatefficientlycombinesthenodestoasingle,unifiedresourcefortheuser,Overallrequirementsforsuchmiddlewarearehighefficiencyandconfigurationflexibility.IntelligentI/O(I2O)isanindustryspecificationthatdefinesaunifrommessagingformatandexecutingmodelforprocessor-enabledcommunicationequipment.Mappingthisconcepttoadistribultedcomputingenvironmentandencapsulatingthedetailsofthespecificationintoanapplication-programmingframeworkallowustoproviderun-timesupportforclusteroperation.Thispapergivesabriefoverviewofaframework.XDAQthatwedesignedandimplementedatCERNfortheCompactMuonSolenoidexperiment'sprototypedataacquisitionsystem.
简介:TheCMSgroupsintheUSAareactivelyinvolvedinseveralgrid-elatedprojects,includingtheDoE-fundedParticlePhysicsDataGrid(PPDG)andtheNSFfundedGridPhysicsNetwork(GriPhyN).Wepresentdevelopmentsof:theGriddataManagementPilot(GDMP)software;aJavaAnalysisStudio-basedprototyperemoteanalysisserviceforCMSdata;toolsforautomatingjobsubmissionschemesforlargescaledistributedsimulationandreconstructionrunsforCMS;modelinganddevelopmentofjobschedulingschemesusingtheMONARCtoolkit;arobustexecutionservicefordistributedprocessors.ThedeploymentanduseofthesetoolsatprototypeTier1andTier2computingcentersintheUSAisdescribed.
简介:TheCMSCollaborationof2000scientistsinvolves150institutionsfrom31nationsspreadallovertheworld.CMSsoftwaresystemintegrationandreleasemanagementisperformedatCERN.CodemanagementisbasedonCVS,withreadorwriteaccesstotherepositoryviaaCVSserver,Softwareconfiguration,releasemanagementtools(SCRAM)arebeingdevelopedatCERN.Softwarereleasesarethendistributedtoregionalcenters,wherethesoftwareisusedbyalocalcommunityforawidevarietyoftasks,suchassoftwaredevelopmentdetectorsimulationandreconstructionandphysicsanalysis.Dependingonspecificapplication,thesystemenvironmentandlocalhardwarerequirements,differentapproachesandtoolsareusedfortheCMSsoftwareinstallationatdifferentplaces.Thispresentationdescribesconceptsandreactialsolutionsforavarietyofwaysofsoftwaredistribution,withanemphasisontheCMSexperienceatFermilab,Installationandusageofdifferentmodelsusedfortheproductionfarm,forcodedevelopmentandforphysicsanalysisaredescribed.
简介:OneofthemostchallengingissuesfacedinHEPinrecentyearsisthequestionofhowtocapitaliseonsoftwaredevelopmentandmaintenanceexperienceinacontinuousmanner.Tocapitaliseinourcontextmeanstoevaluateandapplynewtechnologiesastheyarise,andtofurtherevolvetechnologiesarlreadywidelyinuse,Italsoimpliesthedefinitionandadoptionofstandards,whileensuringreproducibilityandqualityofresults.TheCMSprocessimprovementeffortistwo-pronged.ItaimsatcontinuousimprovementofthewayswedoObjectOrientedsoftware,aswellascontinuousimprovementintheefficiencyoftheworkingenviuronment.Inparticulartheuseandcreationofde-factosoftwareprocessstandardswithinCMShasproventobekeytooursuccessfulsoftwareprocessimprovementprogram.WedescribethesuccessfulCMSimplementationofasoftwareprocessimprovementstrategy,followingISO15504sincethreeyears.WegivethecurrentstatusofthemostimportantprocessesfamiliesformallyestablishedinCMS,andprovidetheguidelineswefollowedbothfortooldevelopment,andmethodologyestablishment.
简介:TheCMSregionalcalorimetertriggersystemdetectssignaturesofelectrons/photons,taus,jets,andmissingandtotaltransverseenergyinadeadtinmesspipelinedarchitecture.Thissystemreceives7000calorimetertreggertowerenergieson1.2Gbanddigitalcoppercableseriallinksandprocessestheminalow-latencypipelineddesignusingcustom-builtelectronics.AttheheartofthesystemistheReceiverCardwhichusesthenewgenerationofgigabitethernetreceiverchipsonamezzaninecardtoconvertserialdatatoparalleldatabeforetransmissionona160MHzbackplaneforfurtherprocessingbycardsthatsumenergiesandidentifyelectronsandjets.Wedescribethealgorithmsandhardwareimplementation,andsummarizethesimulationresultsthatshowthatthissystemiscapableofhandlingtheraterequirementswhiletriggeringonphysicssignalswithhighefficiency.