简介：AsoilpotcultureexperimentwithfoursuppliedPlevels(i.e.P30,P50,P100,P200,representingsupplementalP30,50,100,200mg/kg,respectively)wasconductedtoinvestigateuptakeanduseabilitytoPandZninthericegenotypeswithdifferentP-efficiency,ofwhichricegenotypes508,99011,580,99112werelow-Ptolerantand99056,99012werelow-Psensitive.Low-Ptolerantrice580and99011absorbedmorePthantheothers,andricegenotype580hadstrongeruptakeabilityespeciallyatlow-PlevelsuchasP50andP30.508couldabsorbconsiderableP,andhadthelowestPpercentageofshoot,indicatingithadgoodperformanceinP-useefficiency.ThesethreericegenotypeshadlargerbiomassandlessresponsetochangedPlevelthanricegenotype99112,99056and99012.Ricegenotype99112showedLow-Ptolerancemainlybysacrificingbiomasstomaintainhighrelativegrainyield.TheleastamountofPabsorbedby99056showedithadthelowestPuptakeefficiency,andthehighestPpercentageinshootof99012meantithadthelowestPuseefficiency.Sotheytwoshowedlow-Psensitivity.ZncontentsinshootunderP200,P100andP50weresimilar,butP30increasedZncontentinshootsignificantly.TheZncontentsinshootof99112,99056and99012werehigherthanthoseof508,99011and580,especiallyattilleringstageandbootingstage.AsfortotalZncontentinshoot,Low-Ptolerantricegenotype580hadthelargestamountandfollowedby99011and508,low-Ptolerantricegenotype99012hadthesmallestamountatthethreesamplingstageandfollowedby99056.Furthermore,P/Zninshootof99012wasthehighest,andthatof99056wasthesmallestatthesamePlevel.

简介：Riceblack-streakeddwarfvirus(RBSDV)isarecognizedmemberofthegenusFijivirus,familyReoviridae.Itsgenomehastendouble-strandedRNA(dsRNA)segments(S1-S10),inwhichthefifthgenomesegment(S5)containstwoopenreadingframes(ORFs)withapartiallyoverlappingregion.ThesecondORFofRBSDVS5encodesaviralnonstructuralproteinnamedp5bwithunknownfunction.Torevealthefunctionofp5b,itsgenewasligatedintothebaitplasmidpGBKT7andanexpressionlibrarycontainingricecDNAswasconstructedusingplasmidpGADT7foryeasttwo-hybridassay.Thebaitproteinp5bwasdetectedinyeastbywesternblot,andtheresultofanauto-activationtestshowedthatp5bcouldnotautonomouslyactivatetheexpressionofreportergenesinyeast.Thenthebaitproteinp5bwasusedforscreeningthecDNAexpressionlibrariesofrice.Genefragmentsofsomepivotalenzymesinvolvedinphotosynthesis,respirationandotherimportantmetabolicprocesses,wereidentifiedtointeractwithp5binyeast,suggestingthattheseinteractionsmayplayrolesinsymptomdevelopmentininfectedplants.

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简介：TheexpressionpatternsofOsPIL11,oneofsixputativephytochrome-interactingfactors,wereanalyzedindifferentorgansoftransgenictobacco(Nicotianatabacum).TheexpressionofOsPIL11wasorgan-specificandwasregulatedbyleafdevelopment,abscisicacid(ABA),jasmonicacid(JA)andsalicylicacid(SA).TofurtherexploretheroleofOsPIL11inplantlightsignaltransduction,aplantexpressionvectorofOsPIL11wasconstructedandintroducedintotobacco.Whengrownundercontinuousredlight,OsPIL11-overexpressedtransgenictobaccoexhibitedshorterhypocotylsandlargercotyledonsandleavescomparedtowild-typeseedlings.Whengrownundercontinuousfar-redlight,however,transgenicandwild-typeseedlingsshowedsimilarphenotypes.TheseresultsindicatethatOsPIL11isinvolvedinredlightinducedde-etiolation,butnotinfar-redlightinducedde-etiolationintransgenictobacco,whichlaysthefoundationfordissectingthefunctionofOsPIL11inphytochrome-mediatedlightsignaltransductioninrice.

简介：TheexpressionpatternsofOsPIL11,oneofsixputativephytochrome-interactingfactors,wereanalyzedindifferentorgansoftransgenictobacco(Nicotianatabacum).TheexpressionofOsPIL11wasorgan-specificandwasregulatedbyleafdevelopment,abscisicacid(ABA),jasmonicacid(JA)andsalicylicacid(SA).TofurtherexploretheroleofOsPIL11inplantlightsignaltransduction,aplantexpressionvectorofOsPIL11wasconstructedandintroducedintotobacco.Whengrownundercontinuousredlight,OsPIL11-overexpressedtransgenictobaccoexhibitedshorterhypocotylsandlargercotyledonsandleavescomparedtowild-typeseedlings.Whengrownundercontinuousfar-redlight,however,transgenicandwild-typeseedlingsshowedsimilarphenotypes.TheseresultsindicatethatOsPIL11isinvolvedinredlightinducedde-etiolation,butnotinfar-redlightinducedde-etiolationintransgenictobacco,whichlaysthefoundationfordissectingthefunctionofOsPIL11inphytochrome-mediatedlightsignaltransductioninrice.