简介：MYBtranscriptionfactorsrepresentafamilyofgenesthatincludetheconservedMYBDNA-bindingdomain,andtheyarewidelyinvolvedintheregulationofplantdevelopmentandsecondarymetabolism.Inthisstudy,PartofsequencesoftwoMYBtranscriptionfactorswasdeterminedthroughthecDNAmicroarrayhybridizationandselectionofcDNAlibraryderivedfromtendershoots.Thefull-lengthcDNAsofthegeneswereobtainedwithRT-PCRandRACE,andtheywere1132bpand1020bp,namedasCsMYB1andCsMYB2(GenBankaccessionNo.HQ660373andHQ660374),andcontainedORFsof879bpand675bpencoding292and224aminoacids,respectively.Sequencesanalysisshowedthatthededucedproteinmolecularweightofthetwogeneswere32.9kuand25.4ku,andtheproteinscontainedtwoconservedMYBdomainsneartheN-terminusandaconservedC1motifneartheR3domains.ThededucedaminoacidsequenceofCsMYB1andCsMYB2fromteaplantshowedhighidentitywiththatofotherplants,forinstanceCsMYB1shared57%homologywithMYB1ofGossypiumhirsutumandCsMYB2shared75%homologywithMYBC2ofVitisvinifera.Theresultofrealtime-PCRanalysisshowedthetwogeneswereexpressedconstitutivelyinalltissueswithdifferentexpressionlevels,e.g.therelativeexpressionlevelofCsMYB2inleafwashundredtimeshigherthanthatinroot.Additionally,shadingenhancedCsMYB1expression,whilethetreatmentdidnotaltertheexpressionlevelofCsMYB2.

简介：内细胞团（ICM）和胚胎干细胞（ESCs）一个共同特点，就是对非典型细胞周期的绝对依赖，即G1期被缩短，以保持细胞的自我更新和亚全能特性。

简介：葡萄浆果的颜色是由花色素苷的量来决定的,UFGT控制花色素苷的合成,Myb相关基因通过时空表达调节UFGT,从而使花色素苷在葡萄发育的不同阶段和不同部位表达。不同葡萄品种调节花色素苷的合成的强度和模式的差异造成了有色葡萄品种的果皮有多种颜色,白色葡萄品种果皮是由于没有花色素苷的合成。Myb相关基因家族主要有八个成员,其中MybA的三个种类VlmybA1-1,VlmybA1-2和VlmybA2以及MybB的两个种类VlmybB1-1和VlmybB1-2得到了较深入的研究。

简介：AIM:Toinvestigatetheeffectsofc-mybantisenseRNAoncellproliferationandtheexpressionofc-myb,TGF-β1andα1-Ⅰcollageninculturedhepaticstellatecells(HSC)fromrats.METHODS:Recombinantretroviralvectorofc-mybantisensegene(pDOR-myb)wasconstructed,andthentransfectedintoretroviralpackagecelllinePA317bymeansofDOTAP.ThepseudovirusesproducedfromtheresistantPA317cellswereselectedwithG418toinfectHSCsisolatedfromratlivers.Thecellproliferationwasmeasuredby3-[4,5-Dimethylthiazolzyl]-2,5-diphenyltetrazo-diumbromide(MTT)method.Theexpressionofc-myb,α1-ⅠcollagenandTGF-β1rnRNA,andc-mybproteininHSCswasdetectedwithsemi-quantitivereversetranseription-polymerasechainreaction(RT-PCR)andWestern-blotrespectively.RESULTS:HSCsfromratswereisolatedsuccessfullywiththeviability>98%.InthepDOR-mybinfectedHSCs,thecmybproteinexpression,cellproliferation,andα1-ⅠcollagenandTGF-β1mRNAexpressionwererepressedsignificantlycomparedwiththeircorrespondingcontrolgroups(P<0.01).CONCLUSION:c-mybplaysakeyroleinactivationandproliferationofHSC.c-mybantisenseRNAcaninhibitcellproliferation,α1-ⅠcollagenandTGF-β1mRNAexpression,suggestingthatinhibitionofc-mybgeneexpressionmightbeapotentialwayforthetreatmentofliverfibrosis.