简介：摘要目的建立无水碳酸钠中EDTA-2Na的残留测定方法。方法色谱柱InertsilODS-3，250mm×4.6mm，5μ；流动相0.025mol/L硫酸铵溶液（用硫酸调节pH值至3.0）；检测波长254nm；流速1.0ml/min；进样量50μl。结果EDTA-2Na在0.03～7.49μg/ml浓度范围内具有良好的线性关系，平均回收率为98.02%，RSD为0.82%。结论本法测定无水碳酸钠中EDTA-2Na具有良好的专属性、线性、重复性和准确度，适用于EDTA-2Na的检测。

简介：本文用离子色谱法同时测定了饮用水中的六种阳离子，选择最佳工作参数，提高分析的精密度、准确度。六种离子测定线性范围，相关系数γ>0．9996，相对标准偏差小于0．6％，回收率在98％～103％之间。最低检出限均小于0．004mg／l。

简介：摘要患儿 男，6岁4月龄，因“右下肢跛行5年”于2018年2月就诊于郑州大学附属儿童医院康复医学科。主要临床表现为双下肢无力，右侧显著。腓肠肌肌张力增高，跟腱反射未引出，胸部CT平扫示胸椎侧弯畸形，肌电图示双下肢及右上肢被检神经及肌肉呈神经源性损伤。基因检测示IGHMBP2基因存在c.1202A>G（p.His401Arg）与c.1693G>A（p.Asp565Asn）2个杂合错义变异，属于复合杂合变异。诊断为腓骨肌萎缩症2S型。

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简介：Lactivicin,anovelinhibitorofbacterialcellwallsynthesis,wasisolatedfromtheculturefil-tratesofmicroorganismYK-258andYK-422.Itexhibitsbiologicalactivitiessimilartothoseoftheβ-lactamantibiotics,althoughitdoesnothaveaβ-lactamringinitsmolecule.Sincethediscoveryoflactivicin,hundredsofitsderivativeshavebeensynthesized.Most

简介：<正>Paget’s病也称变形性骨炎或畸形性骨炎,是一种以慢性进行性原因不明的局部骨组织病变为特点的疾病。主要表现为骨的过度吸收,同时伴有骨的过度形成,但新生骨失去正常形态结构。本病以欧美国家发病率较高,我国发病率较低。吴恩惠等曾有报告,而颅

简介：Thisexperimentusedwhole-cellpatch-clamptechniquetoinvestigatethecourseofrecoveryfromuse-dependentblockofNa+channels(Nav1.5)inhumanembryonickidney(HEK)cells,onwhichtoverifytheeffectsofvolatileoilofNardostachychinesisBatal(Gansong).MethodsTwopulsesgeneratedbycomputerfollowedbyarecoverypulseandatestpulse,theintervaldurationbetweenthetwopulsesvariedfrom16msto1s,andholdingpotentialis-80mVto-140mV.ThepeakNa+currentforagivenrecoverytimewasnormalizedtothefullyrecoveredpeakcurrent,andthenormalizedvaluewastheplotasafunctionoftherecoverytimetostudytheeffectsof3ppmconcentrationGansongvolatileoilonrecoveryfromuse-dependentblockofNav1.5inHEK.ResultsItshowedthatGansonggroup,comparingwithcontrolgroup,delayedthetimecoursesofrecoveryfromuse-dependentblock[(33.2±5.77)msforcontrolgroupand(52.5±6.08)msfor3ppmGansonggroup,P<0.05].InthepresenceofGansong,inhibitionoftheNa+currentwasenhancedbyincreasingfrequencyofdepolarizingpulsefrom56.5msto16ms.Inthecontrolgroup,thetimecourseofrecoveryshowedthatrecoverystartedat19.5msandfinishedby36.5ms.InthepresenceofGansong,thetimecourseofrecoveryshowedthatrecoverystartedat36.5msandfinishedby56.5ms.Na+currentsrecoveredfromtheuse-dependentblockvaryingwithholdingpotential(holdingpotential-dependent).ConclusionsTheresultssuggestedthatNa+currentsrecoveredfromtheuse-dependentblockcorrelatedwithpersistenttime,holdingpotential.TheGansongvolatileoilhasinhibitiveeffectontheNa+currentrecovery.

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简介：目的了解福建汉族人群D2S44、D10S28、D4S163VNTR多态性，为亲权关系概率计算提供基因频率。方法采用美国Lifecodes公司提供的VNTR检测试剂盒，应用RFLP技术首次对福建省9个地市103名汉族无关个体进行D2S44、D10S28、D4S163VNTR多态性研究，并将所得基因频率与中国台湾、香港、新加坡等地区进行比较。结果福建汉族人群三位点VNTR呈高度多态性，其杂合率分别为88．6％、86．3％、90．6％，呈孟德尔经典遗传，分布符合Hardy-Weinberg平衡定律。结论福建、台湾、香港、新加坡华人互为比较，D2S44、D10S28位点基因频率没有显著性差别(P>0．500)。

简介：AbstractIntroduction:Darier’s disease is a rare autosomal dominant skin disorder caused by mutations in ATP2A2. Recently, the high prevalence of neuropsychiatric symptoms is frequently reported in Darier’s disease patients.We present a case of patient with concurrence of Darier’s disease and depression and detected the mutations in ATP2A2.Case presentation:A 29-year-old man presented with a 15-year history of brown, harsh keratotic papules on his scalp, face, neck, trunk, axilla, ingunia and upper limbs. Meanwhile, he had persistent depression. He was diagnosed as Darier’s disease according to clinical manifestations and result of histological examination. We sequenced all coding regions of ATP2A2. The mutation c.2993_2994del (p. Val998Alafs*33) in exon 20 of ATP2A2 was detected.Discussion:Darier’s disease has been associated with several extracutaneous manifestations, in particular neuropsychiatric morbidity. Likely gene disrupting mutations in ATP2A2 was reported to have a close relationship to Darier’s disease cases with reported co-occurring neuropsychiatric features. Consistent with the previous reports, an likely gene disrupting mutations was detected in our patient with concurrence of DD and depression.Conclusion:The psychiatric disorders in patients with Darier’s disease should be well appreciated .The underlying mechanism in correlation between the mutation in ATP2A2 and neuropsychiatric phenotypes in Darier’s disease remain unclarified and warrants further investigation.

简介：目的：建立一次性使用真空采血管中的EDTA-Na2的高效液相色谱检测方法。方法：采用VenusilMPC18柱（250mm×4.6mm,5μm）,流动相为甲醇-25%四丁基氢氧化铵-88%甲酸-水（20：1.3：0.6：980）（pH3.5）;检测波长：254nm;柱温：30℃;流速：0.6mL/min。结果：乙二胺四乙酸二钠峰不受干扰,在4μg～100μg/ml浓度范围内线性关系良好（r=1.0000）。高、中、低3种浓度的平均回收率为97.4%。结论：本方法快速简便,专属性强,重复性好,结果准确可靠,适用于多种一次性使用真空采血管中EDTA-Na2的检测。

简介：摘要目的通过温水膀胱冲洗加S2-S4区间腰骶部按摩治疗神经源性尿潴留的课题研究，探讨能够有效改善脑梗死所引发的神经源性尿潴留的方法。方法本课题将2012年3月至2013年2月100例我院收治的脑梗死引发的神经源性尿潴留患者随机分为两组研究组50例，采用液体温度为30-37℃的生理盐水在膀胱排空情况下进行膀胱冲洗加S2-S4区间的腰骶部神经按摩。对照组50例，进行间歇性导尿术，具体方法是嘱患者先自己试行排尿，再插入导尿管排尽尿液，同时可以测残余尿量，根据患者的膀胱残余尿量制定导尿次数。二组疗程均相同，一周为一疗程，间隔一天后，进行第二个疗程，共进行2个疗程后，对数据进行统计分析。结果两组脑梗死引发的神经源性尿潴留患者治疗后评分的比较差异具有统计学意义(P<0.05)，研究组优于对照组。结论本课题对100例我院收治的脑梗死引发的神经源性尿潴留患者，运用温水膀胱冲洗加S2-S4区间神经脊髓段按摩的治疗护理方法与传统的间歇性导尿术比较。通过观察统计，对于促进患者排尿神经功能的恢复及减少并发症、提高患者生活质量，具有确切的疗效。

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简介：Inthepresentstudy,aseriesofnovelnitricoxide-hydrogensulfidereleasingderivativesof(S)-3-n-butylphthalide((S)-NBP)weredesigned,synthesized,andevaluatedaspotentialantiplateletagents.CompoundNOSH-NBP-5displayedthestrongestactivityininhibitingthearachidonicacid(AA)-andadenosinediphosphate(ADP)-inducedplateletaggregationinvitro,with3.8-and7.0-foldmoreeffectivenessthan(S)-NBP,respectively.Furthermore,NOSH-NBP-5couldreleasemoderatelevelsofNOandH2S,whichwouldbebeneficialinimprovingcardiovascularandcerebralcirculation.Moreover,NOSH-NBP-5couldrelease(S)-NBPwhenincubatedwithratbrainhomogenate.Inconclusion,thesefindingsmayprovidenewinsightsintothedevelopmentofnovelantiplateletagentsforthetreatmentofthrombosis-relatedischemicstroke.

简介：目的探讨乙型肝炎病毒pre—S1Ag、pre—S2Ag、HBV—DNA、HBV—M的相关性及其临床意义。方法HBV—DNA采用荧光定量PCR法；HBV—M及pre—S1Ag、pre—S2Ag采用ELISA法，检测96例HBV—DNA阳性感染者血清中pre-S1Ag、pre—S2Ag、HBV—M，同时以30例HBV—M全阴性的健康体检者血清作为对照，并对结果进行统计学分析。结果96例HBV—DNA阳性患者中HBsAg、HBeAg、HBcAb阳性检出率为64．6％；HBsAg、HBeAb、HBcAb阳性检出率为20．8％；HBsAg、HBcAb阳性栓出率为14．6％；pre—S1Ag在96例HBV—DNA阳性标本中检出率为70．8％；pre—S2Ag检出率为79．2％；均明显高于HBeAg的阳性率64．6％，30例对照中未检测出pre—S1Ag、pre—S2Ag及HBV—DNA。结论HBeAg、HBV—DNA、pre—S1Ag、pre—S2Ag之间具有一定的关联性。pre—S1Ag和pre—S2Ag均较HBeAg敏感。pre—S1Ag与pre—S2Ag的栓出率差异无显著性，ELISA检测HBV—M、pre—S2Ag及pre—S1Ag只是表型指标，只能提供HBV感染的间接证据。而HBV—DNA的检测是HBV感染与否的直接证据。HBV—M、pre—S1Ag、pre—S2Ag、HBV—DNA的检测各自有其独特的临床意义。应用pre—S1Ag、pre—S2Ag、HBV—DNA及HBV—M进行联合检测，对HBV感染的早期诊断，了解HBV复制、转归及监测疗效和预后有重要的意义。

简介：RecombinanthumanGABAAreceptorswereinvestigatedinvitrobycoexpressionofcDNAscodingforα1,β2andγ2subunitsinthebaculovirus/Sf-9insectcellsystem,Asingleaminoacidexchangeα1(asparaticacid151toasparaginorα1(threonine149toglutamine)intheN-terminal,extracellularpartoftheα1subunitinducedabout10folddecreaseinanantagonistpitrazepineaffinity.OtherGABAAreceptorligandshadlittledifferenceintheiraffinity.Itwaslikelythat151and149aminoacidresidueswereessentialforthebindingaffinityandefficacyofpitrazepinetoGABAAreceptorcombinationscontainiαααααααααnganα1subunit.

简介：Alzheimer’sdisease(AD)isoneofthemostdevastatingdiseasesaffectingthelifeandhealthofagingpopulation.TwohallmarksofADaresenileplaquesandneurofibrillarytangles,andADiswellknownforthemassivelossofneuronsandimpairedcognitivefunctionsespeciallymemoryloss.Despiteextensivesearchforeffectivetreatment,available

简介：AbstractThe coronavirus disease 2019 (COVID-19) is still causing a wide range of infections and deaths due to the high variability of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Therefore, it is necessary to establish a reliable and convenient pseudovirus-based neutralization assay to develop drug targeted variants of SARS-CoV-2. Based on the HIV-1 backbone, we generated a high titer luciferase (Luc)-expressing pseudovirus packaging system. Three dominant S mutant substitution pseudovirus were also established and identified compared to wide type in hACE2-overexpressing HEK-293T cells (293T-ACE2 cells). Compared to serine protease inhibitor camostat mesylate, the cysteine protease inhibitor E-64d could significantly block all SARS-CoV-2 mutant S pseudovirus infection in 293T-ACE2 cells. Furthermore, the neutralization ability of two antibodies targeted receptor-binding domain (RBD) of SARS-CoV-2 spike protein (S) was evaluated, which showed different inhibition dose-effect curves among four types of S pseudovirus. Overall, we developed a pseudovirus-based neutralization assay for SARS-CoV-2, which would be readily adapted to SARS-CoV-2 variants for evaluating antibodies.

简介：BACKGROUND:GenetherapyforParkinson'sdiseaseisbeingexploredasaneffectivestrategytorestoreandprotectthefunctionofneuronalcellsinthesubstantianigra.Regulationofgeneexpressionisnecessaryforgenetherapytoavoidadverseeffectsduetoexcessivesynthesisoftransgeneproducts.OBJECTIVE:Herewedevelopedrecombinantadeno-associatedvirus(AAV)asaviralvector-mediatedgeneregulationsystembasedonCrerecombinasefusedtothemutatedligand-bindingdomainoftheestrogenreceptor(CreERT2)+inducingagenttamoxifen.InducibleCrerecombinasewasusedtoreducetyrosinehydroxylasegeneexpressionandtopreventtheexcessiveincreaseindopamine.DESIGN,TIMEANDSETTING:Ageneticengineeringinvitrocomparativestudyandrandomizedcontrolledanimalexperiment.ThisstudywasconductedattheGeneTherapyCenter,JichiMedicalSchool,JapanfromJune2002toJune2004.METHODS:ToconstructarecombinantAAVvectorcarryingadopaminesynthasegene.ThetyrosinehydroxylasegenewasinsertedusingaloxPfragmentthatcouldberegulatedbyCrerecombinase.TherecombinantAAVvectorcarryingtheCreERT2genewasco-transducedwithHEK293cellsandthecorpusstriatuminaratmodelofParkinson'sdisease,withinducingagenttamoxifentoregulategeneexpression.MAINOUTCOMEMEASURES:ThelevelsofdopamineandaromaticL-aminoaciddecarboxylase(AADC)activityweredetectedinHEK293cellmediumandinthecorpusstriatuminaratmodelofParkinson'sdiseaseusinghigh-performanceliquidchromatography.ImmunofluorescencedoublestainingwasusedtoobservetyrosinehydroxylaseandCreorAADCco-expressioninHEK293cellmedium.ImmunohistochemicalstainingwasemployedtoobservetyrosinehydroxylaseandAADCexpressionandbehavioralchangesweremeasuredinParkinson'srats.RESULTS:TransfectedAAV-CreERT2andAAVexpressingdopaminesynthesisenzymescouldincreasethesynthesisofdopamineinHEK293mediumandParkinson'sratstriatum(P<0.01)andimprovetherotationalbehaviorofParkin

简介：摘要：随着人类医疗健康及科学技术不断发展，我国医疗科研走在世界前沿，随之而来国民医学营养方面也逐渐成为新的浪潮且被重视起来，这股浪潮为医学营养行业带来了强劲动力的同时，商业模式的概念也贯穿而至，随着时间的推移，公司必须根据多样化趋势调整医学营养的商业模式才能得到最大的受益。因此，本文基于我国对医学营养应用领域及视角来探析S2B2C商业模式。