简介：摘要目的研究LMX１A基因启动子在胃癌细胞中的甲基化状态.方法运用甲基化特异PCR(MSP)法检测３０例原发胃癌和癌旁组织中LMX１A基因的甲基化水平.甲基化修饰后亚硫酸盐测序PCR(BSP)分析胃癌细胞MKN４５细胞LMX１A基因启动子CpG岛甲基化状态.提取细胞RNA,通过实时定量PCR(realtime－PCR)检测LMX１A基因mRNA在不同浓度的DNA甲基化酶抑制剂５’－杂氮－２’－脱氧胞嘧啶(５－aza－dC)处理MKN４５细胞后,LMX１A基因mRNA的改变情况.结果原发性胃癌的甲基化异常检出率是３３％(１０/３０).LMX１A基因在MKN４５细胞中存在高甲基化状态,MKN４５细胞经５－aza－dC处理后LMX１A基因mRNA表达明显上调.结论LMX１A基因在原发性胃癌中存在甲基化异常,说明LMX１A基因基因甲基化异常可能参与胃癌的发生.关键词胃癌细胞;LMX１A基因;DNA高甲基化;５－aza－dCHypermethylationofLMX１AgeneinhumanGastriccancerAbstractObjectiveTodetectthemethylationstatusofLMX１Apromoteringastriccancercells．Methods３０primarygastriccancertissuesandcorrespondingnormaltissuesweredetectedbyMethylation－specificPCR(MSP)method．MethylationstatusofLMX１ApromoterinCRCcelllineMKN４５wasexaminedbyBisulfite－sequencingPCR(BSP)．ExpressionofLMX１AinMKN４５orcellstreatedwithincreasingmountofdemethylationagent,５－aza－dC,wasidentifiedbyRT－PCR．ResultsAberrantmethylationinprimarygastriccancerwas３３％(１０/３０)．HypermethylationofLMX１AwasobservedinMKN４５cells．DecreasedLMX１AmRNAcanberestoredinMKN４５aftertreatmentwith５－aza－dC．ConclusionsThefreＧquentaberantmethylationofLMX１Aisobservedinprimarygastriccancertissues,anddecreasedLMX１AinMKN４５cellsissignificantlycorrelatedwithpKroeymowtoerrdshypermethylation,suggestingthatpromoterhypermethylationofLMX１Amayplayanimportantroleintumorigenesisofgastriccancer．Gastriccancercancer;LMX１A;Hypermethylation;５－aza－dC中图分类号R７３５．２文献标识码A文章编号１００８－６３１５(２０１５)１０－００４２－０１