简介：ＲｅｎｅｗａｌｏｆＣｏｎｔｒｏｌｌｅｒｆｏｒＳＳＣＲａｄｉａｌＰｒｏｂｅｓ￥ＳｏｎｇＨａｉｈｏｎｇａｎｄＣｈｕＺｈｅｎｓｈｅｎｇＳＳＣｍｏｖａｂｌｅｒａｄｉａｌｐｒｏｂｅｓｐｌａｙａｎｉｍｐｏｒｔａｎｔｒｏｌｅｉｎｔｈｅｂｅａｍｔｕｎｉｎｇｏｆｃｙｃ...

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简介：Inthispaper,thefollowingproblemsarediscussed:themountingconditionsofthreeprobes,thetransmissioncharacteristicsofmeasuringsystem,theeliminationofmountingeccentricity,andsoforth.Themeasuresforsolvingtheseproblemsarealsopres-ented.Atlast,theauthorgivessomeideasaboutthesolubilityandthecorrelationsoftheoutputequations.

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简介：ThisstudypresentstheLangmuirandFaradayprobemeasurementsconductedtodeterminetheplumecharacteristicsoftheBUSTLabmicrowaveelectrothermalthruster(MET).Thethruster,designedtooperateat2.45GHzfrequency,isrunwithhelium,argonandnitrogengasesasthepropellant.Forthemeasurements,thepropellantvolumeflowrateandthedeliveredmicrowavepowerlevelsarevaried.Experimentswithnitrogengasrevealedcertainoperationregimeswhereaveryluminousplumeisobserved.Withtheuseofin-house-builtLangmuirprobesandaFaradayprobewithguardring,thrusterplumeelectrontemperature,plasmadensityandioncurrentdensityvaluesaremeasured,andtheresultsarepresented.ThemeasurementsshowthatMETthrusterplumeeffectsonspacecraftwilllikelybesimilartothoseofthearcjetplume.Itisobservedthatthemeasuredplumeionfluxlevelsareverylowforthehighvolumeflowratesusedfortheoperationofthisthruster.

简介：ConformationofL-lysineinaqueoussolutionwasinvestigatedbylanthanideshiftprobes（Dy,Ho,Er,TmandYb）.Reilley’smethodwasemployedtoseparatethecontactanddipolarcom-ponentsofthe13Cparamagneticshifts.ThisstudyrevealsthatCαshifthasthelargestcontactcon-tributionwhiletheothercarbonshiftsaredominatedbythedipolarcontribution.TheaverageoverallconformationofL-lysineinaqueoussolutionisextendedwiththemolecularbackboneintransform.Inthecomplex,lanthanideioncoordinatestothecarboxylgroupwithLn—Obondlength2.2/nandthewholeligandislocatedoutsidethezero-dipolarshiftconeofthelanthanideion.Theelectronicspindensitydistributionontheligandnucleishowsthatthespinpolarizationisthepredominantmech-anismofthecontactinteractionfornucleiincloseproximitytotheboundlanthanideion.

简介：AbstractLuminescence (mostly fluorescence and phosphorescence) probes are a powerful tool widely used in the life sciences research. They can be used, for example, in the quantitative analysis of physiological parameters, visualization of different cell organelles, and measurement of drug transportation. The luminescence intensity and lifetime of these probes are among the main signals recorded and evaluated in these applications. Other reviews have discussed optical probes from the perspective of their luminescence intensity. However, the luminescence lifetime, which depends on the molecular microenvironment but not the probe concentration, is another promising metric for biological sensing and imaging applications. In this review, we aim to introduce the basic strategies of FLIM probe design. We also present applications of these probes, including sensing of intracellular pH, cation/anion concentrations, oxygen levels, biomolecule contents, and physiological parameters, as well as live-cell imaging based on luminescence lifetime. Studies based on FLIM imaging of cells or tissues with endogenous organic molecules are not included in this mini review. With the rapid development of microscopy technology for fluorescence lifetime imaging, fluorescence lifetime-based probes have shown great potential in a variety of biological applications.

简介：AbstractObjective:Immunotherapy is an effective tumor treatment strategy. However, its long treatment cycle limits its wide application across all cancer types. In this study, we optimized upconversion nanoparticles and manganese composite particles with a porous structure as a nanoplatform for synergistic photodynamic therapy (PDT) and photothermal therapy (PTT), and subsequent longer-term immunotherapy.Methods:The morphology, phase, and stability were first characterized to evaluate the biocompatibility of this material. The upconversion and near infrared II luminescence properties of the material and its stimuli-response effect were assessed from the absorbance and photoluminescence spectra. Phototherapy including PDT and PTT was demonstrated in vitro and in vivo, and immunotherapy was used to enhance the phototherapy. This study was approved by the Xi’an Jiaotong University, China (approval No. XJTULAC2020-585) on April 2, 2020.Results:The nanoplatform showed good PDT and PTT effects with high upconversion luminescence, and exhibited a more sensitive glutathione response (detection limit: 55 μg/mL) using fluorescence recovery than that based on absorbance recovery, with the detection range extending up to 1.2 mg/mL. When the surface of the composite particles was modified with an anti-PD-L1 immune checkpoint inhibitor, it targeted A549 lung cancer cells. The resulting immune response enhanced the long-term anti-tumor effect of the therapy, especially in lung cancer patients with high PD-L1 expression.Conclusion:The designed composite can be simultaneously used to detect the glutathione concentration based on luminescence recovery in the tumor cells and as a theranostic nanoplatform for synergistic immuno-phototherapy when combined with an antibody.

简介：WereportontheopticaltrappingandorientationofEscherichiacoli(E.coli)cellsusingtwotaperedfiberprobes.Withalaserbeamat980nmwavelengthlaunchedintoprobeI,anE.colichainconsistingofthreecellswasformedatthetipofprobeI.Afterlaunchingabeamat980nmintoprobeII,theE.coliattheendofthechainwastrappedandorientedviatheopticaltorquesyieldedbytwoprobes.TheorientationoftheE.coliwascontrolledbyadjustingthelaserpowerofprobeII.Experimentalresultswereinterpretedbytheoreticalanalysisandnumericalsimulations.

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简介：二根比色的探针基于为Hg2+的察觉的oxazine-thione1a和1b被设计并且综合。关于在荧光和一种颜色的70褶层改进的探查1a展览在acetonitrile/wate溶剂在Hg2+的增加以后变化。1a对在另外的金属离子上的Hg2+也高度选择。而且，DFT/TDDFT计算被拿向在离开上解释排放反应。

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简介：有害海藻的花蕾最近在在全世界的聚光灯下面，因为他们海洋的环境，水产业，渔业以及公共健康上的否定影响。为原因的种类的快速、精确的鉴定和quantification的方法的发展为花蕾，技术基于分类探针在之中是赞成的themost的警告andmonitoring是必要的。在这研究，二有害水藻，即，最小的Prorocentrum和Kareniamikimotoi被考虑。两个的部分大子单元rDNA(D1-D2)种类是第一放大PCR的，克隆并且定序。获得的序列然后被介绍为基因执行排列分析特定的区域。为每种的三根各自的候选人探针被设计并且过去常由在杂交(鱼)测试的situ执行荧光屏蔽最佳的探查。结果证明探针Pmin0443和Kmik0602为P显示了最好的杂交。最小和K。mikimotoi分别地。两特定(分类)(Pmin0443和Kmik0602)并且控制探针(UniC0512和UniR0499)在我们的实验室与另外的microalgae被用于跨反应的测试。探针Pmin0443和Kmik0602是特定的并且能作为介绍进指向rRNA的技术的分类探针被服务，例如P的鱼，三明治杂交，和DNA-microarray试金。最小和K。mikimotoi以后。最后，有两探查的鱼分析在模仿的地样品上被执行。探针能专门有目标房间井的hybridize，和没有重要差别(p>0.05)在鱼和轻显微镜学(LM)决定的样品的房间密度被观察。都建议探针是特定的并且能为监视两有害水藻被介绍进鱼。

简介：Theeffectsofexerciseondecision-makingperformancehavebeenstudiedusingawidevarietyofcognitivetasksandexerciseinterventions.Althoughthecurrentliteraturesupportsabeneficialinfluenceofacuteexerciseoncognitiveperformance,themechanismsunderlyingthisphenomenonhavenotyetbeenelucidated.Wereviewstudiesthatusedsingle-pulsetranscranialmagneticstimulation(TMS)toprobetheexcitabilityofmotorstructuresduringwhole-bodyexerciseandpresentaframeworktoaccountforthefacilitatingeffectsofacuteexerciseonmotorprocesses.Recentresultssuggestthat,evenintheabsenceoffatigue,theincreaseincorticospinalexcitabilityclassicallyreportedduringsubmaximalandexhaustingexercisesmaybeaccompaniedbyareductioninintracorticalinhibition.Weproposethatreducedintracorticalinhibitionelicitsanadaptivecentralmechanismthatcounteractstheprogressivereductioninmuscleresponsivenesscausedbyperipheralfatigue.Suchareductionwouldrenderthemotorcortexmoresensitivetoupstreaminfluences,thuscausingincreasedcorticospinalexcitability.Furthermore,reductionofintracorticalinhibitionmayaccountforthemoreefficientdescendingdriveandfortheimprovementofreactiontimeperformanceduringexercise.Theadaptivemodulationinintracorticalinhibitioncouldbeimplementedthroughageneralincreaseinreticularactivationthatwouldfurtheraccountforenhancedsensorysensitivity.