简介:Whitespotsyndromevirus(WSSV)-resistantmolecularmarkerswerescreenedfromtheselectivelybrednewvariety‘HuanghaiNo.2’ofFenneropenaeuschinensisusingunlabeled-probehigh-resolutionmelting(HRM)technique.AftertheartificialinfectionwithWSSV,thefirst96deadshrimpsandthelast96survivingshrimpswerecollected,representingWSSV-susceptibleand-resistantpopulations,respectively.Thegenotypesatwell-developed39singlenucleotidepolymorphisms(SNPs)lociwereobtained.AsrevealedintheChi-squaretest,3SNPs,genotypeA/AofcontigC364-89AT,genotypeA/AofC2635-527CAandgenotypeC/TofcontigC12355-592CT,werepositivelycorrelatedwithdisease-resistancetraits.Other2SNPs,genotypeG/GofcontigC283-145AGandgenotypeC/CofcontigC12355-592CT,werenegativelycorrelated.Moreover,analysiswithBlastXprogramfordisease-resistantSNPsindicatedthat3contigs,Contig283,Contig364andContig12355,matchedtothefunctionalgenesofeffectorcaspaseofPenaeusmonodon,peptidetransporterfamily1-likeprotein,and40SribosomalproteinS2ofPercaflavescenswithhighsequencesimilarity.Theresultswillbehelpfultoprovidetheoreticalandtechnicalsupportsformolecularmarker-assistedselectivebreedingofF.chinensis.
简介:Inindirectdrive,reducingpeakintensityofasinglebeamandcontrollingoverlapofmulti-beamsaretwooppositerequirementsforlaserfocalspotdesign.Inthispaper,animprovedlaserspotdesigntechniqueforindirectdrivebuiltuponthegeometricstructuresoflaserpropagationintohohlraumhasbeenintroduced.Theproposedtechniqueisabletogenerateappropriatecontinuousphaseplate(CPP)producingaspecialshapedspotthatcanbalancetheoppositerequirements.ThecorrespondingCPPdoesnotbringdifficultiestothedesignandfabrication.Phaseaberrationsaremoresensitivetothespecialshapedspot;however,itcanbetolerableforthecurrentbeamcontrollevel.
简介:Forestsareamongthemostimportantcarbonsinksonearth.However,theircomplexstructureandvastareasprecludeaccurateestimationofforestcarbonstocks.Datasetsfromforestmonitoringusingadvancedsatelliteimageryarenowusedininternationalpolicyagreements.DatasetsenabletrackingofemissionsofCO2intotheatmospherecausedbydeforestationandothertypesofland-usechanges.TheaimofthisstudyistodeterminethecapabilityofSPOT-HRGSatellitedatatoestimateabovegroundcarbonstockinadistrictofDarabkolaresearchandtrainingforest,Iran.Preprocessingtoeliminateorreducegeometricerrorandatmosphericerrorwereperformedontheimages.Usingclustersampling,165sampleplotsweretaken.Of165plots,81wereinnaturalhabitats,and84wereinforestplantations.Followingthecollectionofgrounddata,biomassandcarbonstockswerequantifiedforthesampleplotsonaperhectarebasis.Nonparametricregressionmodelssuchassupportvectorregressionwereusedformodelingpurposeswithdifferentkernelsincludinglinear,sigmoid,polynomial,andradialbasisfunction.Theresultsshowedthatathird-degreepolynomialwasthebestmodelfortheentirestudiedareashavinganrootmeansquareerror,biasandaccuracy,respectively,of38.41,5.31,and62.2;42.77,16.58,and57.3%forthebestpolynomialfornaturalforest;and44.71,2.31,and64.3%forafforestation.Overall,theseresultsindicatethatSPOTHRGsatellitedataandsupportvectormachinesareusefulforestimatingabovegroundcarbonstock.
简介:摘要目的分析γ-干扰素其自身释放的T细胞斑点去进行试验(T-SPOT.TB)在结核病诊断中存在的意义。方法本文主要选择在2015年1月到12月之间在我院进行住院治疗的肺结核病人共30例,结核性的胸膜炎为30例,结核性的脑膜炎为9例完成T-SPOT.TB相关的检测,同时分别对于他们的血清还有胸腔积液以及脑脊液给予腺苷脱氨酶(ADA)的检测,在医院工作人员的进行身体检查的时候,使用T-SPOT.TB进行检测并且对他们的血清进行ADA检测。结果结核病患者30例,T-SPOT.TB阳性30例,ADA阳性25例,结核性胸膜炎30例,T-SPOT.TB阳性30例,ADA阳性27例,结核性9例脑膜炎,SPOT.TB阳性9例,ADA阳性5例。一家医院共有420名身体检查人员,做T-SPOT检测共291人,阳性54人,阳性率为18.6%,阴性237人,经调查后发现,54人有三人人有结核病治疗,已治愈。其他51人无症状(如下午发热,盗汗等),无迹象(如胸部X线损伤,包括钙化,无胸腔积液)。结论T-SPOT.TB是检测结核病感染最准确的方法之一。灵敏度非常高。所有结核病患者均为阳性,未感染结核病的患者一般均为阳性,可见T-SPOT.TB阳性对结核病的诊断有很强的作用,可是其自身有着一定的假阳性,并通过ADA阳性诊断结核病有一定的假阴性,两者结合,可以显着提高阳性率和特异性但T-SPOT.TB检测阴性,基本可排除结核病(HIV病人除外),其对于结核病的排除有着非常主要的意义。
简介:摘要目的探究T细胞斑点实验(T-spot.TB)在诊断肺结核的价值。方法选取2015年9月~2017年9月在我院收入拟诊肺结核的165例,分成结核病组(95人)和非结核病组(70人),同时选出70例健康的医务工作者做对照组。计量T细胞斑点数,比较T-spot.TB的阳性比率以及对于诊断肺结核灵敏度和特异性的影响。结果结核病组IL-10的表达高于非结核病组和正常组。结核病组T-spot.TB的阳性数值多于非结核病组和正常组(P<0.05),非结核病组和正常组的T-spot.TB的阳性数值没有统计学差别;IL-10表达对于结核病的判别特异性较高(84.1%);T-spot.TB对于结核病的判别敏感性较高(83.3%),采用两者联合检测敏感性最高(90.7%和91.2%)。结论应用T-spot.TB技术可以明显提升结核病诊断的准确性,对于之后进行结核病的医治具有指导价值,值得推广应用。
简介:【句型剖析】1.【原句再现】Johnwasstudyinginhisroomwhenheheardscreaming.约翰正在房间里做功课时,突然听到了尖叫声。【考点剖析】when在此句中意为“这时,突然”,相当于atthis/thattime,常用于以下结构中:bedoing…when…正在做……这时……;beabouttodo…when…正打算做……这时……;beiustgoingtodo…when…正要……这时……;hadiustdone…when…刚做了……这时……;beonthepointofdoing.…when…正要……这时……