简介:摘要目的观察电针百会、太冲、三阴交对鱼藤酮致帕金森(PD)大鼠的中脑黑质超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量的影响,研究针刺治疗PD的作用机制。方法78只Wistar大鼠随机分为正常组、模型组、西药组和电针组,采用颈、背部皮下注射鱼藤酮溶液0.8mg/kg·d制备PD大鼠模型,造模成功后,西药组给予美多巴混悬液灌胃,1.67mg/kg·d,电针组选百会、三阴交、太冲穴,用φ0.30mm×13mm毫针刺入,接韩氏电针仪,采用疏密波,频率疏波2Hz、密波80Hz,强度2.0mA,每次10mg/kg·d,电针正极接一侧三阴交,负极接同侧太冲,双侧相同;西药和电针组均7d/疗程,共治疗2个疗程,疗程间休息1d;除西药组外,其余各组均灌服等体积的生理盐水;除电针组外,其余各组也均采用同一体位固定10min。各组大鼠脱臼处死,取脑,测定中脑黑质SOD活力、MDA含量。结果SOD活力比较模型组低于正常组,P<0.05,电针组高于模型组,P<0.05,西药组与模型组相比,P>0.05;电针组高于西药组P<0.05。MDA含量比较模型组高于正常组,P<0.05,电针组低于模型组P<0.05,西药组低于模型组P<0.05,电针组与西药组相比,P>0.05。结论电针百会、太冲、三阴交具有改善PD大鼠行为学,提高其SOD活力,降低MDA含量的作用。
简介:Humanextracellularsuperoxidedismutase(hEC-SOD)isasecretedtetramericproteininvolvedintheprotectionofahumanbodyfromoxygenfreeradicals.Itsthree-dimensionalstructurehasnotbeenconfirmed,hEC-SODcouldn′tbeexpressedinE.coll.Weconstructedahybridenzyme,whichcomprisestheNterminalandC-terminaldomainsfromhEC-SOD,fusedittohumanCu,Zn-SOD.ThehybridenzymeisexpressedsuccessfullyinE.coli.Further,weanalyzedtheexpressionofhEC-SODinE.colibymRNAdifferentialdisplaying.
简介:TheSOD-likeactivityoffiveCu(Ⅱ)complexes[Cu(HSal)_2·EtOH,Cu(Gly)_2,Cu(Lys)_2,Cu(His)_2,Cu(Try)_2]havebeenstudiedbyusingcytochromeCmethod,aswellaschemilumine-scenceandESRtechnique.Thefoursurfactants(CTAB,SDS,TritonX-100andTween20)werepurifiedandtheircriticalmiceileconcentration(CMC)inphosphatebuffer(pH=7.4)wasmea-sured.TheeffectsoffoursurfactantsonO_2~-havebeeninvestigated.Theco-operativeeffectsof
简介:摘要:近年来,SOD在农业和医药等多个领域都展现出了巨大的应用价值和开发前景。对SOD的功能、抗氧化机制、类型以及生产方式等进行了概述,并重点综述了SOD在食品工业上的应用情况及发展潜力。
简介:采用高效液相色谱(HPLC)等方法测定黑豆、黄豆、绿豆豆芽中异黄酮、多酚的水平,采用1,1-二苯基-2-三硝基苯肼(DPPH)和2,2’-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐(ABTS)方法分析3种豆芽异黄酮和多酚的体外抗氧化活性,研究异黄酮对黑腹果蝇体内超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力的影响。结果表明:黑豆芽的异黄酮、多酚含量最高,黄豆芽次之,绿豆芽最低。4种异黄酮单体中,从黑豆芽中检出染料木苷、大豆苷和黄豆黄苷,从黄豆芽中检出染料木苷和大豆苷,从绿豆芽中只检出大豆苷。3种豆芽异黄酮、多酚提取物的ABTS·+和DPPH·自由基清除能力具有剂量依赖性,拟合方程的相关系数(R2)在0.8957~0.9997区间,提示异黄酮、多酚质量浓度与自由基清除率之间具有较好的量效关系。黑豆芽异黄酮、多酚的IC50值最低,黄豆芽次之,绿豆芽最高,表明黑豆芽的自由基清除能力优于黄豆芽和绿豆芽。3种豆芽异黄酮的IC50值均低于多酚,说明异黄酮的体外抗氧化活性高于多酚。用含有0.5mg/g豆芽异黄酮的培养基饲喂果蝇,可使其SOD、GSH-Px活力明显升高。黑豆芽异黄酮提升果蝇抗氧化酶活力的效果最佳,优于黄豆芽和绿豆芽。本研究为豆芽食品的开发提供了参考依据。
简介:TheSOD-likeactivitiesofcoppersalicylateencapsulatedinneutralpositivelyandnegativelychargedliposomeswerestudiedwithcytochromecmethod.TheresultsshowedthattheSOD-likeactivityofcoppersalicylateencapsulatedinneutralliposomeswasenhancedover3timesmorethanthatofcoppersalicylateinaqueoussolution.However,theactivitydecreasedforcoppersalicylateencapsulatedinpositivelyandnegativelychargedliposomes.Itwasalsofoundthatatexperimentalconcentrationstherewasnoregularityforthechangeofactivityofcoppersalicylateencapsulatedinnegativelychargedliposomes,andcoppersalicylateencapsulatedinliposomeswithmorenegativechargescouldpromotethedismutationofsuperoxideanionfreeradicals,however,socouldcoppersalicylateencapsulatedinliposomeswithfewernegativechargesonlyinlowerconcentrations.