简介：Atrialnatriureticpeptide(ANP)isapeptidehormonethathaspotentnatriuretic,diuretic,vasodilator,sympatholytic,andrenin-andaldos-terone-suppressingactivitiesandisinvolvedintheregulationofvolumeandelectrolytebalanceandbloodpressure.Further,ANPhasalsobeenshowntoinhibitcellulargrowth,proliferationandinduceapoptosisinavarietyofcelllines,includingvascularsmoothmusclecellsandcardiacmyocytes.RecentstudieshavedemonstratedthatANPisnotonlyinvolvedinbloodpressureandvolumehomeostasisbutalsointhedirectregulationofcardiacgrowth.Weandotherinvestigatorshavedemonstratedtheexistenceofnatriureticpeptidereceptorsintheheartandcardiaccells,suggestingthatANPhasdirectactionsoncardiactissue.SeveralrecentinvivostudieshavesuggestedthatstatementofANPisinverselyrelatedtocardiacgrowth/hypertrophy.TransgenicmiceoverexpressingANPhavelowerheartweightandbloodpressurethanwildtypemice.Conversely,we

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简介：C类型natriuretic肽(CNP)是调停的本地规章的因素的一种最新发现的类型它通过specic的生物效果，膜界限natriuretic肽receptor-B(NPR-B)。最近的研究证实了CNP是仔细与男繁殖功能有关。这研究的目的是通过不同方法在人的呼喊的精子决定CNP/NPR-B的分发，然后在精子功能invitro上评估CNP的影响(例如immunolocalization，实时聚合酶链反应和西方的污点)，例如活动性和acrosome反应。人的精液样品从同意的施主被收集为normozoospermia满足了世界健康组织的标准。我们的结果证明CNP的specic受体NPR-B在头和精子的前端尾巴的膜的acrosomal区域是局部性的，并且没有在人的精子的CNP的信号。与控制相比，CNP能在精子活动性和acrosome反应导致signicant剂量依赖者增加。在摘要，CNP/NPR-B能影响精子活动性和acrosome反应，因此调整男性的繁殖功能。CNP可以是在调整精子功能的一个新关键因素。

简介：ObjectivesTostudytherelationshipbetweenplasmaBNPandcardiacstructural,functionalchangesafterAMI,andtoevaluatetheapplicationofplasmaBNPdeterminationinpredictingtheearlyphaseofventricleremodelingafterAMI.MethodsAMIgroupincluded37patients,allpatientunderwentstrictmedicaltreatment;32healthyindividualswereenrolledascontrols.PlasmaBNPwasdeterminatedbyimmunofluorescence.EchocardiogramexaminationwasusedtodeterminateLVEF,V_E,V_A,V_E/V_A,LVEDd,LVEDV;Allpatientsunderwenttheexaminationsat72h,30daysand90daysafterAMIoccurred,respectively,andwerecomparedwithhealthycontrols.ResultsIncomparingtocontrols,PlasmaBNPinAMI72h,30daysand90dayselevatedsignificantly.LVEDdandLVEDVwereincreasedsignificantlyinall3phasesofAMI,andEF,V_E/V_Aweresignificantlylowerthanincontrols.CorrelationanalysisrevealedthatplasmaBNPlevelin72hafterAMIwaspositivelycorrelatedtoLVEDd,LVEDV,andwaspositivelycorrelatedtoEF,V_E/V_Ain30daysand90daysafterAMI.ConclusionsPlasmaBNPlevelin72hafterAMIisapowerfulindextopredictleftventricleremodelingandventriclefunctionasplasmBNPlevelispositivelycorrelatedtoventricleremodeling;loweringtheplasmBNPlevelmightbeusedasanendpointfortreatmentofheartfailure.

简介：Objective:Toobservethetherapeuticeffectofelectroacupuncture(EA)onplasmaangiotensin(Ang*.Ⅱ),aldosterone(ALD)andatrialnatriureticpolypeptide(ANP)contentsinexperimentalcerebralinfarctionrabbitsforanalyzingtheunderlyingmechanismofacupunctureinamelioratingbloodsupplyofthebraintissue.Methods:Atotalof80rabbitswererandomizedintocontrol(n=8),pseudo-operation(n=24),model(n=24)andEA(n=24)groups.Cerebralinfarctionmodelwasestablishedbyinfusionofself-thrombusintothecarotidartery.EA(1mA,2Hz)wasappliedto'Baihui'(百会GV20)and'Shuigou'(水沟GV26)for30min,onceevery12hours.PlasmaAng-II,ALDandANPcontentsweredetectedwithradioimmunoassaymethod.Inthelater3groups,bloodsamplesweretakenat6h,24hand48haftercerebralischemia.Results:Comparedwithcontrolandpseudo-operationgroups,Ang-IIandALDcontentsofmodelgroupat6h,24hand48haftercerebralischemiaincreasedsignificantlywhileplasmaANPofthe3time-coursesofmodelgroupdecreasedconsiderably(P<0.01).Incomparisonwithmodelgroup,resultsshowedthatAng-IIandALDcontentsofEAgroupdecreasedsignificantlywhereasANPlevelofEAgroupincreasedstrikingly(P<0.01).Conclusion:ElectroacupuncturehastheeffectsofraisingplasmaANPlevelandloweringplasmaAng-IIandALDincerebralinfarctionrabbits.

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简介：AbstractBackground:Atrial natriuretic peptide (ANP) and its natriuretic peptide receptors A (NPR-A) and C (NPR-C) are involved in the regulation of physiological and pathophysiological process of blood pressure. The present study aimed to determine the role of NPR-C in the development of salt-sensitive hypertension.Methods:The Dahl salt-sensitive (DS) and salt-resistant (DR) rats were used in this study. Animals were matched according to their age and weight, and then placed on either a high-salt (HS, 8%) or a normal-salt (NS, 0.4%) diet for 6 weeks randomly using random number table. The systolic blood pressure (SBP), plasmatic sodium concentration (PLNa), urinary sodium excretion (UVNa), and serum creatinine concentration (Scr) were measured. The concentration of ANP in blood and tissues (heart and kidney) was detected by enzyme-linked immunosorbent assay. The expression of ANP, NPR-A, and NPR-C in kidney was evaluated with western blot analysis. Regarding renal redox state, the concentration changes in malondialdehyde (MDA), lipofuscin, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox), and nitric oxide synthase (NOS) in kidney were detected by a spectrophotometric method. The kidney damage was evaluated using pathological techniques and the succinodehydrogenase (SDHase) examination. Furthermore, after an intra-peritoneal injection of C-atrial natriuretic peptide (ANP)4-23 (C-ANP4-23), an NPR-C receptor agonist, the SBP, biochemical values in blood and urine, and renal redox state were evaluated. The paired Student’s t test and analysis of variance followed by the Bonferroni test were performed for statistical analyses of the comparisons between two groups and multiple groups, respectively.Results:The baseline SBP in all groups was within the normal range. At the end of the 6-week experiment, HS diet significantly increased the SBP in DS rats from 116.63 ± 2.90 mmHg to 162.25 ± 2.15 mmHg (t = -10.213, P < 0.001). The changes of SBP were not significant in DS rats on an NS diet and DR rats on an NS diet or on an HS diet (all P > 0.05). The significant increase of PLNa, UVNa, and Scr related to an HS diet was found in both DS and DR rats (all P < 0.05). However, significant changes in the concentration (t= -21.915, P < 0.001) and expression of renal ANP (t= -3.566, P = 0.016) and the expression of renal NPR-C (t = 5.864, P = 0.002) were only observed in DS hypertensive rats. The significantly higher desmin immunochemical staining score (t = -5.715, P = 0.005) and mitochondrial injury score (t = -6.325, P = 0.003) accompanied by the lower SDHase concentration (t= 3.972, P = 0.017) revealed mitochondrial pathologic abnormalities in podocytes in DS rats with an HS diet. The distinct increases of MDA (t= -4.685, P= 0.009), lipofuscin (t= -8.195, P= 0.001), and Nox (t= -12.733, P < 0.001) but not NOS (t = -0.328, P = 0.764) in kidneys were also found in DS hypertensive rats. C-ANP4-23 treatment significantly decreased the SBP induced by HS in DS rats (P < 0.05), which was still higher than NS groups with the vehicle or C-ANP4-23 treatment (P < 0.05). Moreover, the HS-induced increase of MDA, lipofuscin, Nox concentrations, and Nox4 expression in DS rats was significantly attenuated by C-ANP4-23 treatment as compared with those with HS diet and vehicle injection (all P < 0.05).Conclusions:The results indicated that the renal NPR-C might be involved in the salt-sensitive hypertension through the damage of mitochondria in podocytes and the reduction of the anti-oxidative function. Hence, C-ANP4-23 might serve as a therapeutic agent in treating salt-sensitive hypertension.

简介：ObjectivesToinvestigatethepossibleroleofamino-terminalpro-brainnatriureticpeptide(NT-proBNP)intheoccurrenceofatrialfibrillation(AF)aftercoronaryarterybypassgrafting(CABG).MethodsThisstudygroupincluded70consecutivepatientsscheduledforelectiveoff-pumpCABG.Thepatientswithejectionfraction(EF)lessthan0.30,historyofAF,useofclassIorIIIantiarrhythmicdrug,implantedpacemaker,postoperativemyocardialinfarctionorchestreopeningforpericardialtamponadewereexcluded.PreoperativeandpostoperativeserumNT-proBNPlevelsweremeasuredbyradioimmunoassaytechnique.ResultsPostoperativeAFoccurredin15patients(21.4%);thesepatientshadsignificantlyhighermedianNT-proBNPlevelswhencomparedwiththosewithoutAFaftertheoperation(P<0.01).Usingmultivariatelogisticregressionanalyses,anincreaseinNT-proBNPlevelafterCABGwasfoundtobeindependentlyassociatedwithAF(OR=3.78,95%IC=1.81～4.89,P<0.01).Increasedage,diabetesmellitus,preoperativeuseofβ-blocker,proximalrightcoronaryarteryinvolvement,andlongeroperationtimewerealsoassociatedwithAF.ConclusionsTheseresultsindicatedthatAFwasassociatedwithhigherNT-proBNPconcentrationsafteroffpumpCABG;theincreaseinNT-proBNPafterCABGmayplayanimportantroleintheoccurrenceofAFaftertheoperation.ThefurtherstudiesareneededtodefinethereasonthatleadtohigherNT-proBNPconcentrationsamongthepatientswhopresentAFafteroffpumpCABG.

简介：【摘要】目的：研究冠心病急性心肌梗死病人采用冠脉介入治疗对心室重塑和BNP指标的作用。方法：将我院2018年7月至2019年12月就诊的57例冠心病急性心肌梗死病人作为实验对象，依据不同的治疗方案进行分组。将28例采用常规治疗的病人分为参照组，29例采用冠状介入治疗的病人则为实验组，记录对比两组治疗前后BNP指标的变化情况，治疗后心功能相关指数。结果：经过治疗可见，实验组病人BNP指数（324.63±208.58）比参照组BNP指数（

简介：【摘要】目的：研究冠心病急性心肌梗死病人采用冠脉介入治疗对心室重塑和BNP指标的作用。方法：将我院2018年7月至2019年12月就诊的57例冠心病急性心肌梗死病人作为实验对象，依据不同的治疗方案进行分组。将28例采用常规治疗的病人分为参照组，29例采用冠状介入治疗的病人则为实验组，记录对比两组治疗前后BNP指标的变化情况，治疗后心功能相关指数。结果：经过治疗可见，实验组病人BNP指数（324.63±208.58）比参照组BNP指数（