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简介：提取高质量的植物基因组ＤＮＡ是顺利开展ＲＡＰＤ分析研究的一项重要基础工作，在目前比较广泛使用的植物基因组ＤＮＡ的提取方法中，改进的Ｄｏｙｌｅ和Ｄｏｙｌｅ等人的ＣＴＡＢ植物基因组ＤＮＡ提取法被证实为一种简单、高效的方法，值得推荐．更多还原

简介：Nanogproteinisexpressedintheinteriorcellsofcompactedmorulaeandmaintainedtillepiblastsbutdownregulatedbyimplantationstage.Itisalsoexpressedinembryonicstemcells,embryoniccarcinomacellsandembryonicgermcellsbutdisappearedindifferentiatedEScells.Inthisstudy,wehaveisolated,sequenced,andperformedthefirstcharacterizationoftheNanogpromoter.Thetranscriptionstartsitesweremappedbyprimerextensionanalysis.TwopromoterregionswerefoundupstreamthetranscriptionstartsitesandtheexpressionofmajorNanogpromoter/reportergeneconstructisabolishedindifferentiatedF9ECcellsascomparedtotheundifferentiatedcounterpart.Wealsoshowedthataputativeoctamermotif(ATGCAAAA)isnecessaryforthemajorpromoteractivity.GelshiftandsupershiftassaysshowedthatOct-1,Oct-4andOct-6proteinselectivelybindtotheoctamermotif.

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简介：细菌的谷物的随便有机体的隔离和察觉上的调查米饭腐烂在19972006期间被进行。在2006，六病原的细菌的紧张从米饭(OryzasativaL.)的样品原来在海南省生产了然后在浙江省种了的二粒无症状的种子被孤立，中国。他们作为是细菌的谷物的原因的有机体的Burkholderiaglumae被识别与四标准参考紧张相比由生理的特征，殖民地形态学，致病力测试，Biolog，丰满的酸甲基酉旨(名声)分析和RAPD-PCR米饭腐烂。有B的感染，这被证实。处于看种子的所谓的健康的glumae。

简介：Inthisstudy,athermophilicoil-degradingbacterialconsortiumKO8-2growingwithinthetemperaturerangeof45-65℃(with55℃beingtheoptimumtemperature)wasisolatedfromoil-contaminatedsoilofKaramayinXinjiang,China.Denaturinggradientgelelectrophoresis(DGGE)showedthattherewereninestrainsincludedinKO8-2,whichoriginatedfromthegeneraofBacillus,GeobacillusandClostridium.Theyallbelongedtothermophilicbacteria,andhadbeenpreviouslyprovedasdegradersofatleastonepetroleumfraction.ThecrudeoildegradedbyKO8-2wasanalyzedbyinfraredspectrophotometry,hydrocarbongrouptypeanalysisandgaschromatography.TheresultsindicatedthatthebacterialconsortiumKO8-2wasabletoutilize64.33%ofsaturates,27.06%ofaromatics,13.24%ofresinsandtheoilremovalefficiencyreachedupto58.73%at55℃whentheoilconcentrationwas10g/L.DetailedanalysisshowedthatKO8-2wasabletoutilizethehydrocarboncomponentsbeforeC19,andthen-alkanesrangingfromC20-C33weresignifi-cantlydegraded.TheratiosofnC17/PrandnC18/Phwere3.12and3.87,respectively,beforedegradation,whereasafterdegradationtheratiosreducedto0.21and0.38,respectively.Comparedwiththecontrolsample,theoilremovalefficiencyinKO8-2compostingreactorreached50.12%afteradegradationdurationof60days.

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简介：Satellitecells(SCs)hasbeenshowntobethesourceofmyogenicprecursorcellsresponsibleformusclefiberrepairandregenerationduringatrophy,afterinjuryorneuromusculardisorders.RecentstudiesdemonstratedthefeasibilityofautologousSCstransplantationforrepairofmyocardialnecrosis.

简介：Circulatingtumorcells(CTCs)arethecancercellsthatcirculateintheperipheralbloodafterescapingfromtheoriginalormetastatictumors.CTCscouldbeusedasnon-invasivesourceofclinicalinformationinearlydiagnosisofcancerandevaluationofcancerdevelopment.Inrecentyears,CTCresearchhasbecomeahotspotfieldwhereinmanynovelCTCdetectiontechnologiesbasedonmicrofluidicshavebeendeveloped.Greatadvanceshavebeenmadethatexhibitobvioustechnicaladvantages,butcannotyetsatisfythecurrentclinicalrequirements.Inthisstudy,wereviewthemainadvancesinisolationanddetectionmethodsofCTCbasedonmicrofluidicsresearchoverseveralyears,proposefivetechnicalindicatorsforevaluatingthesemethods,andexploretheapplicationprospects.Wealsodiscusstheconcepts,issues,approaches,advantages,limitations,andchallengeswithanaimofstimulatingabroaderinterestindevelopingmicrofluidics-basedCTCdetectiontechnology.

简介：IsolationofHelicobacterpylori(HP)isthegoldstandardforthediagnosisofHPinfection.However,theconventionalisolationmethodistedious,expensiveandtroublesome,andisnotsuitableforuseingeneralpractice.Wehavedevelopedasimple,low-costandeffectivemethodfortheisolationofHP.TwobiopsyspecimensfromeachpatientwereinoculatedonaHPselectivebloodplate,andplacedinaspeciallydevisedplasticjarcoveredair-tightlywithalid.Afterfillingwithamixturegas(CO210%,O25%,N285%),

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简介：ActinomycetesinfivemarinesedimentscollectedfromtheArcticOceanatdepthsof43to3050mwerecultivatedusingavarietyofmedia.Atotalof61actinomycetecolonieswithsubstratemyceliaonlywereobserved,andnocolonieswithaerialmyceliawereobservedunderaerobicconditionsat15℃.Fromthesecolonies,28wereselectedtorepresentdifferentmorphologicaltypes.Denaturinggradientgelelectrophoresis(DGGE)wasusedtocheckthepurityofisolatesandselectrepresentativesforsubsequentsequencing.Phylogenticanalysesbasedonnearlyfull-length16SribosomalRNAgene(rDNA)sequencesindicatedthattheactinomycetesisolatedwereaccommodatedwithingenusRhodococcusoffamilyNocardiaceae,genusDietziaoffamilyDietziaceae,generaJanibacterandTerrabacteroffamilyInstrasporangiaceaeandgeneraKocuriaandArthrobacteroffamilyMicrococcaceae.Oneofthestrains(P27-24)fromthedeep-seasedimentatdepthof3050mwasfoundtobeidenticalin16SrDNAsequence(1474/1474)withtheradiation-resistantKocuriaroseaATCC187Tisolatedfromair.Morethanhalfoftheisolatesshowedthesimilaritiesrangingfrom99.5%to99.9%in16SrDNAsequencetodibenzofran-degrading,butyl2-ethylhexanoate-hydrolysisingandnitrile-metabolizingactinomycetes.Allthestrainsisolatedwerepsychrotolerantbacteriaandgrewbetteronthemediapreparedwithnaturalseawaterthanonthemediapreparedwithdeionizedwater.Threeofthem(Dietziasp.P27-10,Rhodococcussp.S11-3andRhodococcussp.P11-5)hadanobligategrowthrequirementforsalt,confirmingthatthesestrainsareindigenousmarineactinomycetes.

简介：半活跃的水坝pers被使用在对还原剂基础隔离土木工程学结构的地震反应。在现在的学习，用可变扩大的一个新半活跃的抑制系统将被调查为适应基础隔离。它使用在系列连接与的一台新奇可变扩大设备(VAD)一被动更潮湿。VAD能够生产多重扩大因素，各相应于一个不同扩大状态。力量从更潮湿根据当前的扩大状态被放大到结构，它经由明确地定制到系统的一个半活跃的控制算法被选择“s唯一的抑制特征。为adaptivebase隔离表明VAD潮湿系统的有效性，数字模拟为三被进行，易于远、近地的地面的七故事的base-isolatedbuildings打手势。结果显示系统能与孤立底的大楼相比在反应完成重要减小没有更潮湿。建议系统也被发现表现很好与相比一典型半活跃更潮湿。

简介：由对改进隔离表演的需要激发了，许多研究研究与非线性的特征在隔离乾上被执行了。基于他们的阶段肖像的形状，如此的设备能作为也被设置单稳态或双稳态的隔离乾。这篇论文集中于在一样的刺激下面调查这二个班的相对表演。力量能递送被用来测量隔离表演，它以播送力量的比率的RMS被定义到刺激力量。当系统受到泛音刺激时，在隔离的力量能递送的最大的减小用伪变化，这被发现--零僵硬被完成。当系统受到随机的刺激时，它有伪的一样的效果--零僵硬。进一步，最佳抑制能与僵硬被改变并且有最小的价值。

简介：AbstractViral isolation in cell cultures has been regarded for decades as the "gold standard" for the laboratory diagnosis of influenza viral infections. Not all viral strains could be isolated from clinical samples. This study aimed to quantify the viral load in the samples before isolation to save working time and improve working efficiency. Four hundred samples from patients with influenza-like cases were confirmed pdmH1N1 positive (200 cases) and B Victoria (BV) positive (200 cases) by whole-genome sequencing and analyzed by ddPCR for viral load in samples before isolation, and isolation results were verified by hemagglutination (HA) assay and hemagglutination-inhibition (HI) tests. Probit regression analysis was used to calculate the isolation viral load limit with a 95% probability level by SPSS 19.0 software. The results showed that the isolation limit of viral load was 4.9 × 104 (95% CI: 2.5 × 104-9.0 × 104) copies/mL for pdmH1N1 and 1.9 × 104 (95% CI: 7.8 × 103-3.6 × 104) copies/mL for BV. The isolation rate of clinical samples is positively correlated with the viral load in clinical samples, which can be used for viral culture, providing important guidance for daily work.

简介：AbstractPatients with acute infectious respiratory illness may emit bio-aerosols containing pathogens to infect susceptible hosts, including other patients and healthcare workers. This study aimed to design the pediatric isolation (PI) bed and evaluate its purification effect on aerosols and microorganisms in the air in experiment rooms and verify the general wards' purification efficiency. The PI bed is connected to a purification device that surrounds the child's head to prevent contaminants' spread into the room. We test the purification effect of the PI bed in the experiment room and verified the purification effect of the PI bed in the hospital. The results showed that PI beds in the demonstration ward presented purifying effects on both smoke particulates and microorganisms like Staphylococcus albus (S. albus) and H1N1 virus. The Aerosol in the room in the hospital was then detected to validity the purification rate. Therefore, Isolation beds could be used in hospitals to reduce the risk of nosocomial infection to protect the health of doctors, patients, and visitors.

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简介：SolublemalewormantigenofSchistosomajaponicurn(Sj)wasinvestigatedfordevelopmentofnewvaccinecandidate.SDS--PAGEandWesternblottingwereperformedtocomparethedifferencebetweensolubleantigensfromwormsofdifferentsex.MicevaccinationwiththetestingpurifiedproteinwasfollowedbySjcereariaechallengetodetecttheprotectiveeffectagainstSj.Sixteenbandswereseenforthesolublemalewormantigenand12forthefemaleworm.Inaddition,adistinctbandof44.6kDafromthemalewormantigenwasobserved,anditsantigenicitywasdemonstratedbyWesternblotting.This44.6kDaproteincouldinducesignificantwormandeggreductionrateinmice(39.31%,41.98%,P<0.001).Inthisstudya44.6kDaproteinwasisolatedandpartiallycharacterized.Itsantigenicity,immunogenicityandthepartialimmuneprotectionsuggestitspotentialvaccinecandidteagainstSj.

简介：ObjectivesToestablishamethodforhighyieldmesenchymalstemcellscollection,aswellasaculturemethodforidentifyingmesenchymalstemcellsfromtheswineadipose-derivedmesenchymalstemcell(ADMSC).MethodsSwineADMSCswereisolatedfromfattissuewithcollagenase,followedbyinductionofdifferentiationtoosteogenic,adipogenicandchondrogrniccells.ThesurvivalcurveoftheADMSCatthe37oCand38oCweremeasuredusingWST-1CellProliferationAssayReagent.ResultADMSCsisolatedwithcollagenasefromswineneckfattissuegeneratedastableuniformappearanceafterthesecondgeneration.Thepassageperiodwasfivedays.ADMSCcoulddifferentiateintoosteogenic,adipogenicorchondrogrniccellsunderdifferentcultureconditions.Thehighestgrowthratewasachievedat38oCinthisstudy.ConclusionSwineADMSCshavethepotentialtodifferentiateintoosteogenic,adipogenicorchondrogrniccells,andtheymaybeappropriatefortransplantationforbothresearchandclinicalpurpose.

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