简介：客观:处于pathophysiological条件工作在试验性的动物学习mitochondrial氧化还原作用状态易感知地检测mitochondrial的早符号，例如局部缺血。方法:菸碱腺嘌dinucleotide(磷酸盐)的荧光，或NAD(P)H，在为cardiomyocytes的重要ATP供应负责的mitochondrial呼吸的主要电子施主，为mitochondrial功能的非侵略的荧光灯的探查被学习。在由由幽灵似地解决的相关时间的单个光子数的紫外脉搏的激光二极管和察觉(TCSPC)的生活cardiomyocytes追随者刺激的NAD(P)H荧光的检查，基于荧光系列和一生的同时的测量。结果:在生活老鼠cardiomyocytes表演的NAD(P)H荧光腐烂的动态特征至少一个3-exponential腐烂模型与0.4-0.7ns，1.2-1.9ns和8.0-13.0ns一生，是必要的描述cardiomyocyteautofluorescence(AF)。联系腐烂的系列(DSA)在cardiomyocytes揭示了NADH分子的4张幽灵似地不同的人口的存在与光谱在为短一生的水池的470nm最大第一次，并且在为中间、长一生的水池的450nm，470nm和490nm的排放山峰。由酉同类身体的增加的mitochondrialNADH内容比率提高了AF紧张，没有重要变化在荧光灯一生。毒鱼藤素，建筑群的禁止者我mitochondrial呼吸的链，增加了AF并且弄短平均荧光一生。二硝基酚(DNP)，mitochondrial的一个解开的代理人氧化phosphorylation，降低的AF，拓宽光谱在520nm的肩膀并且增加了平均一生。这些效果，比得上在集中并且处于在vitro的NADH的除氢作用的率的变化，也在局部缺血模仿的conditions.Conclusion下面被检验:我们的调查结果从NADH期望两个conformationalNADH变化和精力转移的贡献到lipoamide脱氢酶(LipDH)界限黄素，为了解释，观察到荧光kinetics.Presented幽灵似地决定荧光一生途径在生活为mitochondrialNAD(P)H的分析提供有希望的新工具

简介：Anewopticalfiberfluorometricthermometerbasedonthetemperaturedependenceofthefluorescencelifetimeofphosphorisdescribed.Thephase-lockeddetection(PLD)systemisusedtomeasurefluorescencelifetime.Thecharacteristicsofthermmeterarediscussedandtheexperimentresultsaregiven.

简介：Vesiclecanbepreparedfromaqueousmixturesofsimplecommerciallyavailable,single-tailedcanonicandanionicsurfactants.Inthiswork,theI3/I1value,Ie/Imvalue,andfluorescencelifetimeofpyreneindifferentsystems(seethepreparationofsamples)weredetermined.Hieessentialaffectingfactorsintheformationofvesiclecanbededucedfromtheobtainedresults.Itshowedthatlargevesiclemustformnaturallybeforesonicationin0.082MoctyltrimethylammoniumbromideandsodiumlauratepH=9.2aqueoussolution.Whileaftersonication,onlysmallvesicleexists,whichcanbeprovedfurtherthroughelectronmicroscope.

简介：控制并且操作单个fluorophores的荧光具有对为它在改进分子的photonics和分子的电子学的表演的潜在的使用的最近的年的大兴趣，例如在基于单个分子的器官的轻射出的设备，单个光子来源，器官的地效果晶体管，和探针或传感器。这评论显示出单个器官的分子的荧光排放怎么能用金属性的nanostructures和electric-field-induced电子转移的本地电磁的领域被修改。荧光开关的Electric-field-induced荧光调整，磁滞现象，和成就详细被讨论。

简介：Objective:Infectionofhumanpapillomavirusincondylomaacuminatum(CA)wasdetectedbyrealtimefluorescencequantitativePCR(FQ-PCR)technique.Methods:SpecimensofCA-DNAquantificationfrom94caseswereexaminedbyrealtimeFQ-PCRtechniqueand32caseswerecomparedwiththesamemethodafter10-daystreatment.Results:CA-DNAwasfoundinallpatients,withanaverageof4.0×10^6copies/ul.After10daysoftreatment,theaveragewas2.1×10^5copies/ul.TherewasasignificantdifferenceintheaverageamountofCA-DNAbeforeandafterthetreatment.Conclusion:RealtimeFQ-PCRisagoodmethodforexaminingCA-DNAamountanditcandirectthetreatmentofCA.

简介：Anovelfluorescencelifetimeimagingmicroscopy(FLIM)workingwithdeepUV240–280nmwavelengthexcitationshasbeendeveloped.UV-FLIMisusedformeasurementofdefect-relatedfluorescenceanditschangesuponannealingfromfemtosecondlaser-inducedmodificationsinfusedsilica.ThisFLIMtechniquecanbeusedwithmicrofluidicandbiosamplestocharacterizetemporalcharacteristicsoffluorescenceuponUVexcitation,acapabilityeasilyaddedtoastandardmicroscope-basedFLIM.UV-FLIMwastestedtoshowannealingofthedefectsinducedbysilicastructuringwithultrashortlaserpulses.Frequency-domainfluorescencemeasurementswereconvertedintothetimedomaintoextractlongfluorescencelifetimesfromdefectsinsilica.

简介：三倍的螺旋状的结构的热稳定性在骨胶原生合成，功能和降级起一个关键作用。CD技术被利用描绘合成骨胶原的热稳定性模仿肽。因为它的便宜乐器学，低操作费用，容易的操作，和高敏感，荧光光谱学广泛地全世界与容易的存取被使用。这里，我们开发了一个其他的荧光方法检测骨胶原的热稳定性模仿肽。我们证明了那荧光光谱学能测量骨胶原的热稳定性在不同情形下面与低集中模仿肽。自我熄灭的试金将极大地帮助的这高度敏感的荧光骨胶原的性质模仿的顺序依赖者的研究肽，和它在决定象骨胶原，collectins，adiponectin，巨噬细胞scavenger和C1q那样的三倍的螺旋系统的热稳定性的应用程序有大潜力。

简介：α-andβ-cyclodextrinsconsistingofsixandsevenglucoseresiduesrespectively,havelipophiliccavitieswithdifferentinnerdiameters.Theyformhost-guestinclusioncomplexeswithhydrophobicorganicandorganometallicguestmoleculesinaqueoussolution.Thesehost-guestcomplexeshaveprovedtobeexcellentmodelsystemsforstudyingthenatureofnoncovalentbondingforcesinaqueousmedia.TheyhaveprovidedvaluableinsightsintothehydrophobiceffectandLondondispersionforcesandaregoodmodelforunderstandingthespecificityofenzymesubstrateinteractions[1]Evidencefortheformationofinclusioncomplexeshavebeenprovidedfromcalovimetrictitration[2]NMR[33],circulardichroism[4],UV[1]andfluorescencespectra[5]andconductometricmethod[6]etc.Herewereportanewfluorimetricmethodforastudyonthereactionofthehost-guestinclusioncomplexesofcyclodextrinwithphenols.Dissociationconstants(Kd)oftheinclusioncomplexesofsomephenolswithα-β-cyclodextrinareestimatedbasedonthevariationofthefluorescentintensityandmodifiedHarad'equations.

简介：Thioglycolicacid(TGA)-stabilizedCdTenanocrystals(NCs)werepreparedwithsodiumtelluriteastelluriumsource,whichavoidsthecumbersomeprocessesassociatedwithH2TeorNaHTesources.FluorescentCdTe/SiO2compositesweresynthesizedbyasol-gelmethodwithouttheexchangeofsurfaceligands.ThephasestructureofCdTeNCswasinvestigatedbyX-raydiffractometry.Forcomparison,somecharacterizationsweredoneforboththeCdTeNCsandthecomposites.CdTeNCsandCdTe/SiO2compositeswerecharacterizedwithTEM,digitalcameraandfluorescencespectrophotometer.ThestabilityofCdTeNCsandthecompositeswereinvestigatedinphosphate-bufferedsaline(PBS)bufferandthefluorescentpropertiesofthecompositeswerediscussedindetail.

简介：OurexperimentsshowthatexternalfocusingandinitiallaserenergystronglyinfluencesfilamentgeneratedbythefemtosecondTi–sapphirelaserinair.Theexperimentalmeasurementsshowthefilamentlengthcanbeextendedbothbyincreasingthelaserenergyandfocallengthoffocusinglens.Ontheotherhand,theplasmafluorescenceemissioncanbeenhancedbyincreasingthelaserenergywithfixedfocallengthordecreasingthefocallength.Inaddition,thecollapsedistancemeasuredexperimentallyarelargerthanthecalculatedonesowingtothegroup-velocity-dispersioneffect.Inaddition,wefindthatthelinewidthsofthespectrallinesfromN2isindependentoffilamentpositions,laserenergiesandexternalfocusing.

简介：在氧气体的处理以后，包含胺组的小分子能射出荧光。氧化被相信在荧光中心的形成起一个重要作用。比作以前的结果，小分子和大分子可能有一样的荧光中心。

简介：

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简介：Fluorescenceliftimeimaging(FLIM)ofmodifiedhydrophobicbodipydyesthatactasfluorescentmolecularrotorsshowsthatthefluorescencelifetimeoftheseprobesisafunctionofthemicroviscosityoftheirenvironment.Incubatingcellswiththesedyes,wefindapunctateandcontinuousdistributionofthedyeincells.Theviscosityvalueobtainedinwhatappearstobeendocytoticvesiclesinlivingcellsisaround100timeshigherthanthatofwaterandofcellularcytoplasm.Time-resolvedfluorescenceanisotropymeasurementsalsoyieldrotationalcorrelationtimesconsistentwithlargemicroviscosityvalues.Inthisway,wesuccessfullydevelopapracticalandversatileapproachtomapthemicroviscosityincellsbasedonimagingfluorescentmolecularrotors.

简介：荧光刺激和可溶的有机物(WSOC)样品从20提取了的46水的平均分子的重量在东方中国玷污类型被决定。它被发现所有样品分享类似的光谱学。一种好线性关系不过在350～450nm的范围在全部的器官的碳和刺激之间存在器官的碳的内容和样品的pH在一个宽范围变化。在相对刺激紧张和WSOC和FA的平均分子的重量之间的重要关联都没被发现，但是部分关联为WSOC样品作为控制因素与pH变得重要。相对刺激紧张显示出在学习区域从南方增加到北方的一个一般趋势。pH价值可能在调整起一个重要作用荧光灯WSOC的空间变化。

简介：Ourrecentstudiesconcerningthebindingofionicsurfactantsonoppositelychargedpolyelectrolytesobservedwithfluorescencetechniquesarereviewed.Thecationicsurfactantscetyltrimethylammoniumbromide(CTAB),dodecyltrimethylammoniumchloride(DTAC),andnonionicsurfactantoctaethyleneglycolmonododecylether(C12E8)wereallowedtobindonanionicpoly(2-acrylamido-2-methylpropanesulfonicacid)(PAMPS)anditspyreneand/ornaphthalenelabeledcopolymers.TherelativeexcimeremissionintensityIE/IMofacationicprobe1-pyrenemethylaminehydrochloridewerechosentomonitorthebindingprocessandtheconformationchangeofsurfactant-boundpolyelectrolytes.The1:1aggregationofpolyelectrolyte-CTABwithrespecttothechargewasfoundaslongastheCTABconcentrationwasslightlyhigherthanitscriticalaggregationconcentration(CAC).TheintermolecularNRETindicatedthattheCTAB-boundpolyelectrolytesaggregatedtogetherthroughthehydrophobicinteractionbetweentheCTABtails.However,neither1:1polyelectrolyte-DTACaggregationnorintermolecularaggregationofDTAC-boundpolyelectrolytewasobservedowingtoitsweakerhydrophobicityof12carbonatomsinthetail,whichisshorterthanthatofCTAB.Asknownfromthefluorescenceresults,nonionicsurfactantC12E8didnotbindontheanionicpolyelectrolytes,butthepresenceofPAMPSpromotedthemicelleformationforC12E8attheCACslightlybelowitscriticalmicelleconcentration(CMC).ThesolidcomplexofdansyllabeledAMPScopolymer-surfactantexhibitedadecreaseinlocalpolaritywithincreasingchargedensityofthepolyelectrolyteorwithalkanetaillengthofthesurfactant.SAXSsuggestedalamellastructurefortheAMPScopolymersurfactantsolidcomplexeswithalongperiodof3.87nmforCTABand3.04nmforDTAC,respectively.

简介：一种荧光眼纤维的温度计基于红宝石和吸收玻璃样品被设计。眼--纤维温度测量探查基于红宝石被开发。这个系统对在0℃to的范围的温度测量特别地能适应130℃。更加改进的表演在这台新设备被看见。到带的罐头的驱动电流容易通过控制电路在要求的定义界限以内被保留。

简介：Introduction.Poly(N-vinylcarbazole)(PVCZ)hasreceivedmuchattentionasarepresentativephotoconductivepolymerandextensivestudiesonmechanical,physical,chemicalandelectricalpropertieshavebeenreported'’.Ingeneral,thePVCZobtainedbyradicalandordinarycanonpolym...

简介：荧光灯颜料，基于光或染料的electrooptical性质，是主要部件在荧光灯涂层和墨水。在这研究，三种染料(玫瑰精B，轻格林SF微黄色，香豆素)与四不同比率(2.5?wt%，3?wt%，3.5?wt%，4?wt%)作为发光体被采用，并且三聚氰胺甲醛(MF)树脂被用作治好树脂准备荧光灯在不同颜色的颜料。Fourier变换红外线的光谱学和X光检查diffractometry被执行分析结构荧光灯颜料。扫描电子显微镜学和粒子尺寸分发被用来介绍形态学荧光灯颜料。紫外力并且荧光光谱被用来表明光性质。这能被结束，当玫瑰精B可能是为从粒子尺寸的看法在三种染料之中打印墨水的准备的最好时，香豆素颜料在MF树脂拥有了连续结构。TG结果介绍了那所有颜料显示出好热稳定性，它可能在高速度打印拥有潜在的申请工业。

简介：Mitoxantrone,aclinicallyusefulantitumourantibioticforleukaemiaandbreastcancer,hasreceivedmoreattentions.Inthispaper,theinteractionbetweenmitoxantroneandcalfthymusDNAisinvestigatedbyRamanandfluorescencespectroscopies,andthebindingsiteofmitoxantronetocalfthymusDNAisexplored.TheresultsshowedthatmitoxantroneinteractswithcalfthymusDNAbasesbytheintercalationofanthracyclineintothebasepairplaneofadenine(A)andthymine(T),anditresultsinthedisruptionofthehydrogenbondsbetweencalfthymusDNAbases,andthusthecalfthymusDNAdouble-strandcanbedisruptedintotheB-formDNAdouble-strandsegments.