简介：Objective:ToconfirmwhetherMycoplasmapneumoniae(MP)arepresentinreproductivetractofSTDpatientsinChina.Methods:ApplicationofnestedPCR(nPCR)andDNAsequencingtotestsamplesofurethral/vaginalswabswithMPcultureconfirmationofseveralnPCRpositivepatients.Results:74of786STDpatientswerepositiveforMPbynPCR,witharateof9.4%.Ofthe484malepatients,10.5%werepositive,andamongthe302femalepatients,7.6%werepositive.Therewasnosignificantdifferencebetweenthem(P>0.05).Of12casesofMPpositivesamplesbynPCR,4caseswerefirstgenerationculture-positive,andoneofthempassedtothenextgenerationsuccessfully.DNAsequencingwasperformedonthenPCRproductofoneswabsampleandoneMPcultureisolation.ThedeterminedsequencewasidenticaltothetypicalMPstrain.Conclusion:InChina,MParepresentinreproductivetractofbothmaleandfemaleSTDnatients.