简介:目的:探讨CT影像诊断技术应用于高血压脑出血临床诊疗效果,分析其高死亡率的原因,为高血压脑出血的临床治疗提供依据。方法选取2011年3月至2013年3月我院收治的60例高血压脑出血患者为研究对象,应用GEBrightspeed9916层螺旋CT行CT影像检查,回顾性分析其影像诊断结果。结果60例患者均行CT扫描。其中,血肿位置位于脑叶5例,无死亡案例;位于壳核45例,死亡4例(8.88%);位于丘脑7例,死亡3例(33.33%);位于小脑3例,死亡1例(33.33%)。有破入脑室10例,死亡例数3例(33.33%);无破入脑室50例,6例死亡(12.00%)。出血小于50ml的患者共计42例,死亡4例(9.52%);出血量为50-100ml15例,死亡4例(26.66%);出血量大于100ml3例,死亡1例(33.33%)。脑中线结构移位小于1cm51例,死亡6例(11.76%);脑中线结构移位大于1cm9例,死亡3例(33.33%)。以上指标之间两两比较,差异显著,P〈0.05,具有统计学意义。结论CT影像诊断技术应用于高血压脑出血患者的临床诊断中,便捷、快速、精确性高,值得临床推广。
简介:BackgroundTheprognosticvalueofserumC-reactiveprotein(CRP)inpatientswithinfectiveendocarditis(IE)isnotwellelucidated.ThisstudyaimedtoevaluatetheusefulnessofCRPinpredictingtheoutcomeofIE.MethodsTwohundredninty-sixpatientsfrom2009to2012intheDepartmentofCardiologyatGuangdongGeneralHospitalwerescreenedanddividedintosurgicalandconventionaltreatmentgroups.CRP,whitebloodcell(WBC),erythrocytesedimentationrate(ESR)andotherclinicaldatawereobtainedwithfollow-upfor12months.ResultsTwohundredthirty-sixpatientswereassignedtoreceivesurgerytreatmentwhile60patientsreceivedconventionaltreatment.Inthesurgerygroup,thelevelofCRPinthedeathpatientswassignificantlyhigherthanthatinthesurvivalpatients(P<0.001).TheareaunderthecurveofROCwasabout0.749(SE0.064,P=0.005,95%CI,0.624-0.874)andthecut-offpointofCRPwas23.8mg/L.Inconventionalgroup,therewassignificantdifferencebetweendeathandsurvival(P<0.001).TheareaunderthecurveofROCwasabout0.701(SE0.095,P=0.032,95%CI,0.515-0.888)andthecut-offpointsofCRPwas65.6mg/L.TherewerenosignificantdifferencesinWBCandESRbetweensurgeryandconventionalgroups.ConclusionAmoreaggressivesurgicalinterventionresultsinabetteroutcomeoverconventionaltreatmentandCRPcouldbeservedasapredictivemarkerforadverseoutcomeinIEpatients.
简介:目的评估低密度脂蛋白胆固醇(LDL—C)/高密度脂蛋白胆固醇(HDL—C)比值对经皮冠脉介入(PcI)术后患者心血管事件的预测价值。方法选择急性冠脉综合征(ACS)并予前降支置入支架的患者119例,依据血浆LDL—C/HDL—C比值将患者分为3组,随访1年,评估三组患者心血管事件发生率,以及各危险因素与心血管事件发生率的关系。结果①与LDL—C/HDL—C比值较低的两组相比,比值较高组患者体重指数、女性患者百分率、吸烟人数及糖化血红蛋白、高敏C反应蛋(hs—CRP)、总胆固醇和LDL—C水平均明显升高,而HDL—C水平和他汀类药物使用率则较低(P〈0.05)。②第1组风险(HR)1.04,95%可信区间(c,)0.98-1.08,第2组HR1.16,95%C11.08-1.20,第3组HR1.27,95%C11.19~1.36(P〈0.05)。随着LDL—C/HDL—C比值的升高,PCI术后1年患者心血管事件发生率也逐渐升高(P〈0.05)。③Cox比例风险回归模型提示,LDL—C/HDL—C比值对PCI术后心血管事件风险的预测价值优于其他危险因素。结论LDL—C/HDL—C比值对PCI术后患者1年内心血管事件再发具有一定的预测价值。
简介:目的探讨冠脉内支架术的临床效果及小C臂X光机的可行性.方法对1998年5月~2001年6月间在小C臂X光机下60例冠脉内支架植入的患者进行回顾性分析,评定手术成功率、并发症及远期疗效.结果60例患者植入83个支架:DeNovo支架植入48个,Suboptimal11个,Restenosis2个,Bailout2个.成功81个:前降支45处病变植入40个支架,右冠脉29处病变植入27个支架,左回旋支16处病变16个支架;支架植入的成功率98.9%.本组无急性或亚急性血栓形成严重出血并发症.随访1~36个月,5例再发心绞痛冠脉造影证实,2例原支架植入处再狭窄,另3例无再狭窄,系多到血管病变,其中1例发生心源性猝死.结论冠脉内支架手术成功率高,并发症低;有效抗凝抗血小板及调脂治疗,是冠脉内支架治疗效果的重要内容;冠脉内支架术在小C臂X光机下是安全可行的.
简介:ObjectivesTostudythedepressiveeffectoftheantisenseoligonuceotides(ASODN)ofc-mycandproliferatingcellnuclearantigen(PCNA)ontheproliferationofVSMC.MethodsTakingtheVSMCobtainedfromrataortathoracaliscultured4~8generationasresearchobject.Theobjectsweredividedintothreegroupstocarryoutcontrolstudy:controlgroup,PCNAASODNgroupandc-mycASODNgroup.TheASODNs'workingconcentrationallwere1:50.ThedepressiveeffectofASODNonVSMCproliferationwasinvestigatedbycellcounting,MTTand3H-TdRincorporationassay;PCNAandc-mycexpressionweredetectedbyimmunohistochemicalmethodaftertransferringPCNAandc-mycASODNintoVSMC.ResultsPCNAandc-mycASODNcouldinhibittheproliferationofVSMCsignificantly,comparedwithcontrolgroup(P<0.05).②TransferringPCNAandc-mycASODNintoVSMCobtainedsuccessfully;thecorrespondinggenewasinhibitedobviously;comparedwithcontrolgroup(P<0.05).ConclusionsPCNAandc-mycmightplayaconsiderableroleintheVSMCproliferationprocess.ThecorrespondinggenecouldbedepressedsuccessfullyaftertransferringPCNAandc-mycASODNintoVSMC,andthentheproliferationofVSMCwassloweddown.Thisstudypresentedabeneficialproposalandtheoreticalfundamentforatherosclerotictreatment.
简介:Bothendotheliallipasegene(LIPG)584C>T(rs2000813)polymorphismandalcoholconsumptionmodulateserumlipidlevels.Buttheirinteractionsonserumlipidprofilesarenotwellknown.ThepresentstudywasundertakentodetecttheinteractionsofLIPG584C>Tpolymorphismandalcoholconsumptiononserumlipidlevels.GenotypingoftheLIPG584C>Twasperformedin763nondrinkersand520drinkersaged15-85.Interactionsbetweenthegenotypesandalcoholconsumptionwereassessedbyusingacross-productterm.Thelevelsofserumtotalcholesterol(TC),triglyceride(TG),high-densitylipoproteincholesterol(HDL-C),apolipoprotein(Apo)AI,andtheratioofApoAItoApoBwerehigherindrinkersthaninnondrinkers(P<0.01forall).Therewasnosignificantdifferenceinthegenotypicandallelicfrequenciesbetweennondrinkersanddrinkers.ThelevelsofserumTC,HDL-CandApoAIinnondrinkersweredifferentamongthethreegenotypes(P<.05-.01).ThesubjectswithCTgenotypehadhigherserumTC,HDL-CandApoAIlevelsthanthesubjectswithCCgenotype.ThelevelsofserumHDL-CandApoAIindrinkersweredifferentamongthethreegenotypes(P<.001andP<.05;respectively).TheindividualswithTTgenotypehadhigherserumHDL-CandApoAIlevelsthantheindividualswithCCandCTgenotypes.ThelevelsofTCinnondrinkerswerecorrelatedwithLIPG584C>Tallele(P<.05),whereasthelevelsofTGandHDL-CwereassociatedwithLIPG584C>Talleles(P<.05)andgenotypes(P<.05);respectively.ThepresentstudysuggeststhatthesubjectswithTTgenotypebenefitedmorefromalcoholconsumptionthanthesubjectswithCTandTTgenotypesinincreasingserumHDL-CandApoAIlevels.
简介:backgroundBonemarrowmesenchymalstemcells(BMSCs)canbeisolatedandculturedtomanypassages.However,StemcellsincludingBMSCsquicklyundergosenescenceinculture.Thecellsenescenceandmulti-directionaldifferentiationhavehamperedproducingBMSCsinquantitywiththeirundifferentiatedstate.Inthisstudywereportanaturalcompound,vitaminC(Vc),maintainsBMSCsstemproperty.MethodsHumanBMSCswereisolatedfrombonemarrowandpurifiedby1.073g/mLdensitygradientcentrifugation.50ng/mLVcwereaddedtoBMSCsfordifferenttimepoint.FlowcytometrywasusedtodetectcellsurfacemarkersofBMSCswithorwithoutVctreatment.BMSCsproliferationwasanalyzedbyMTTassay.PCR(polymerasechainreaction)andreal-timePCRwereusedfordetectingc-kit,nanog,andOct-4genesexpressionlevels.DNAmethyltransferase(Dnmt)1andDnmt3blevelswerealsodetectedbyreal-timePCR.ResultsFlowcytometryshowedthatafterVctreatmentfor6h,thesurfacemarkersofBMSCswerealmostunchanged.VcincreasedtheproliferationactivityofBMSCsfrom6hto24h.PCRshowedtheexpressionofc-kit,nanog,andoct-4geneswereobviouslyincreasedinVctreatedgroupthancontrolgroupat12h.Real-timePCRshowedthatthelevelofc-kit,nanog,andoct-4geneswereunregulatedfrom6hto12hcomparedwithcontrolgroup.VcalsoincreasedDnmt3bbutnotDnmt1geneexpression.ConclusionsOurresultsshowedVcactsatleastacceleratesBMSCsproliferationandmaintainsstemcellproperty.Inourstudy,wehighlightedamethodofimprovingthespeedofBMSCsgenerationandprovidedadditionalinsightsintothemechanisticbasisofpreventingBMSCssenescence.
简介:BackgroundInthisstudy,weaimedtoevaluatetheimpactofabnormalglucose,lipidandCystatin-ConthevirtualPvectorcharacteristics,whichhaven’tbeenreportedinpreviousstudies.Methods204ofnon-diabetesmellitus(NDM),130ofDM(type2)and39ofimpairedglucosetolerance(IGT)patientswereconsecutivelyandretrospectivelyrecruited.Weselectedaone-minutelengthofelectrocardiogramat4AMforanalysis.Afteraseriesofcalculatingalgorisms,weobtainedthevirtualplanarPvectorparameters.ResultsTherewerenosignificantdifferencesinFPV,FPA,RSPV,RSPA,HPVandHPAgroups.Afteradjustingconfoundingfactors,theregressioncoefficients(RC)wereestimatedasfollow:forFPV,femalegender(RC-0.21,P=0.02),triglyceride(RC-0.09,P<0.01),RVOT(RC0.03,P=0.02);forRSPV,femalegender(RC-0.21,P<0.01),triglyceride(RC-0.10,P<0.01),averageheartrate(RC0.01,P=0.02);forHPV,triglyceride(RC-0.08,P<0.001),LDL(RC-0.19,P<0.01),ApoB(RC0.67,P<0.01);forRSPA,Btypeofblood(RC-22.06,P=0.02),Cystatin-C(RC-72.79,P=0.02),thicknessofinterventricularseptum(RC3.70,P=0.01).Cystatin-CwassuggestedasacurerelatedtoRSPA,andthecut-offpointwas1.6mg/L.TherewerenosignificantriskfactorsassociatedwithFPAandHPA.TherewasnodifferenceinvirtualPvectoramongDM,IGTandNDMgroups.ConclusionIncreasedlevelsoflipidandCystatin-CsignificantlyimpactthecharacteristicsofvirtualPvector,whereasglucosedoesnot.Thesechangesmaycomefromahigherlowvoltageatrialareaandabnormalorientationofatrialdepolarization.