简介：Objective:Toevaluatethetreatmentforpatientswithmajorvascularinjuriesassociatedwithtraumaticorthopedicinjuries.Methods:Atotalof196patients,agedfrom4-67yearswiththemeanageof29.88years,hadmajorvascularinjuriesassociatedwithtraumaticorthopedicinjuriesandweretreatedinourhospitalinaperiodof44years.Themostcommonmechanismoftraumawasblunttrauma(67.3%),openinjuriesaccountedfor32.4%and54.5%oftheinjurieswerelocatedinthelowerextremities.Thevascularinjuryfrequentlyhappenedinthefemoralartery(26.7%)andpoplitealartery(20.3%).Thetreatmentprincipleconsistedofaggressiveresuscitation,Dopplerimagingandstableboneinternalfixationwithsubsequentvascularrepairanddebridement.Thevascularrepairforinjuriesincludedend-to-endanastomosis(80cases,39.6%),interpositionalveingraft(94,46.5%),vasculardecompressionthroughfracturedistraction(18,8.9%),arterialligation(6,3.0%),veinpatch(2,1.0%),bypassgraft(2,1.0%),venousrepairincludingautogenousveingraft(9,24.3%)andligation(28,75.7%)andprophylacticalfasciotomy(15,7.4%).Postoperativeamputationwasperformedin16cases(16.3%).Results:Nointraoperativedeathwasobservedandallfracturesunitedwithin6months.Limbsweresalvagedin180patients(91.8%).Amongthesepatients,earlycomplicationswerefoundin19patients(9.7%)andlatecomplicationswereobservedin8patients(4.1%).Conclusions:Awell-organizedapproach,basedonaspecifictreatmentprinciple,notonlyimprovesclinicaloutcomebutalsodoesgoodtoexcellentfunctionalrecoveryforpatientswithsevereorthopedicinjuriesandconcomitantvascularlesion.

简介：锁骨下的脉管的损害说明所有脉管的损害的1%-5%。如果没及时发现或迅速设法，锁骨下的脉管的损害是可能的引起出血性的吃惊甚至死亡。从通过2003的8月的1998的三月，有锁骨下的脉管的损害的15个盒子的一个总数在我们的部门被对待。细节在这份报告的redescribed。

简介：Objective:Toinvestigatetheneointimaformationandtheexpressionofmonocytechemoattractantprotein-1(MCP-1)andtumornecrosisfactor-α(TNF-α)incuff-inducedvascularinjuryinmousemodel,andtoexaminetheeffectofangiotensinIItype1receptor(AT1)blocker,olmesartan,onMCP-1andTNF-αexpressionandconsequentlyvascularremodeling.Methods:Vascularinjurywasinducedbypolyethylenecuff-placementaroundthemousefemoralartery.SomemiceweretreatedwithAT1receptorblocker,olmesartan,atthedoseof3mg*kg-1*day-1withanosmoticminipump.Neointimaformationandtheproliferationofvascularsmoothmusclecells(VSMCs)weremeasuredbymorphometricanalysisandbromodeoxyuridine(BrdU)incorporation.MCP-1andTNF-αexpressionwasdetectedbyWesternblotandimmunohistochemicalstaining.Results:Weobservedneointimaformation14daysaftercuffplacementaswellasVSMCsproliferationinthemediaandneointima.CuffplacementalsoinducedMCP-1andTNF-αexpressioninthemediaandneointimathattheVSMCsspecificallyexisted.Treatmentofmicewitholmesartanatadoseof3mg*kg-1*day-1,whichdidnotinfluencesystolicbloodpressure,significantlydecreasedneointimaformationandtheproliferationofVSMCs.OlmesartanalsoinhibitedMCP-1andTNF-αexpressionintheinjuredarteries.Conclusions:OurresultsdemonstratethatblockadeofAT1receptorinhibitsMCP-1andTNF-αexpressionandtherebyimprovesvascularremodeling.

简介：在与对脉管的损伤的照顾和治疗感兴趣的从在整个世界的同事一起的知识的国际交换的精神，我们在脉管的损伤上从西方的世界提供选择历史的思考。而有很多个关键个人和许多事件，在我们的写对我们重要，我们不实质上对被另外的文化并且，特别地写汉语的知道任何东西。中国外科医生在第一之中在切断的极限的重新种植园高度成功，这很好在全世界被认出，修理受伤动脉和静脉。另外，我们认识到在世界的另外的部分有贡献，它不一起对我们众所周知。从说的阿拉伯语的贡献世界分开因为有周期的简短参考，出现在脑海。我们提供我们希望将有至少一位中国外科医生，将为我们提供在对在西方的世界外面设法脉管的损伤的重要历史的贡献上分享他们的观点的利益的观点，并且，特别地，英语说文学。再次，我们用说的阿拉伯语鼓励我们的同事向我们提供他们发展的观点和脉管的损伤的管理的世界。

简介：Objective:Toinvestigatethebindingcharacteristicsofendothelialcell(EC)withLPSfreefromtheparticipationofserumfactors.Methods:LaserconfocalmicroscopewasemployedintheobservationofthebindingofECwithFITC-LPS.TheKDandthebindingsitesofeachECwerecalculatedbyradioligandbindingassayofreceptors(RBA)using[3H]-LPS.Results:ThebindingofECwithLPSwassaturable,timeandconcentrationdependentanditcouldbecompetedwithoverdosedLPSofthesametype.Thefluorescencemainlydistributedincytoplasm,especiallynearthenucleus,whichcouldalsobestained.Conclusions:TheremightbesomespecificLPSbindingsitesexistingonEcsandLPScouldfunctionintracellularily.

简介：ObjectiveTo评估未耕作的脂肪质导出stromal的潜在的效果腱healing.MethodsTwenty上的脉管的部分称2.5-3.0kg的五只成年雄的新西兰白兔子被使用。当脂肪质的组织和剩余的施主被划分成控制和治疗组，五只兔子被使用。损害模型被单方的腱切断术通过中间完成三分之一深数字的屈肌腱。立即在缝术以后修理，也新鲜的stromal从脂肪质的织物或安慰剂的酶的消化的脉管的部分intratendinously分别地在治疗和控制组在腱树桩被注射。有演员组的固定在外科以后被继续二个星期。动物在外科以后在八个星期被牺牲，腱经历了组织学，immunohistochemical，和机械评估。量、质的数据的统计分析用变化和Mann-Whitney的单程的分析被估计[/-test，表明的优异纤丝状的线性和连续性，和在处理的减少的由脉管形成组织的respectively.ResultsHistological评估显示了改进组织并且neotendons改变。Immunohistochemistry在骨胶原表明了重要增加我在处理的表示组。最终的负担和精力吸收能力显著地两个都与学习显示出的在场的controls.ConclusionThe相比在对待房间的修理被增加未耕作的脂肪质导出stromal的那intratendinous注射脉管的部分结果在改进了腱修理和它的结构、机械的性质能是为对待腱损害的一种有效形式。

简介：

简介：Objective:Tostudytheeffectofnitricoxide-inducedtyrosinephosphorylationoflarge-conductancecalcium-activatedpotassium(BKCa)channelαsubunitonvascularhyporesponsivenessinrats.Methods:Atotalof46Wistarratsofeithersex,weighing250g±20g,wereusedinthisstudy.Modelsofvascularhyporesponsivenessinducedbyhemorrhagicshock(30mmHgfor2hours)invivoandbyL-arginineinvitrowereestablishedrespectively.Thevascularresponsivenessofisolatedsuperiormesentericarteriestonorepinephrinewasobserved.TyrosinephosphorylationofBKCaαsubunitwasevaluatedwithmethodsofimmunoprecipitationandWesternblotting.Results:Inthesmoothmusclecellsofthesuperiormesentericarteries,theexpressionofBKCaαsubunittyrosinephosphorylationincreasedfollowinghemorrhagicshock,andL-argininecouldinduceBKCachannelαsubunittyrosinephosphorylationinatime-anddose-dependentmanner.L-NAME(Nω-nitro-L-arginine-methyl-ester),anitricoxidesynthetaseinhibitor,couldpartlyrestorethedecreasedvasoresponsivenessofthesuperiormesentericarteriesafterhemorrhagicshockinrats.Down-regulatingtheproteintyrosinephosphorylationwithgenistein,awidely-usedspecialproteintyrosinekinaseinhibitor,couldpartlyimprovethedecreasedvasoresponsivenessofthesuperiormesentericarteriesinducedbyL-arginineinvitro,whileup-regulatingtheproteintyrosinephosphorylationwithNa3VO4,aproteintyrosinephosphataseinhibitor,couldfurtherdecreasethenitricoxide-inducedvascularhyporesponsiveness,whichcouldbepartlyamelioratedby0.1mmol/Ltetrabutylammoniumchloride(TEA),aselectiveBKCainhibitoratthisconcentration.Conclusions:NitricoxidecaninducethetyrosinephosphorylationofBKCaαsubunit,whichinfluencesthevascularhyporesponsivenessinhemorrhagicshockratsorinducedbyL-arginineinvitro.

简介：Objective:Toinvestigatetheexpressionandpatternofvascularendothelialgrowthfactor(VEGF)anditsfetalliverkinase-1(Flk-1)receptorinspinalcordanddorsalrootgangliaafterneurotomyofsciaticnerveinrats.Methods:Forty-fiveadultmaleWistarratsweredividedrandomlyintoacontrolgroup(n=5)andanexperimentalgroup(n=40).ThebilateralsciaticnervesoftheratsintheexperimentalgroupunderwentneurotomyandtheL4-L6spinalcordandthecorrespondingdorsalrootgangliawereharvestedrespectivelyat8hours,and1,3,5,7,10,14and21days(8subgroupswith5ratseach)afteroperation.Theratsinthecontrolgrouponlyunderwentanexposureofsciaticnervewithoutneurotomy.ImmunohistochemistryandimageanalysiswereusedtostudytheexpressionofVEGFanditsFlk-1receptor.Results:BothVEGFandFlk-1receptorexpressedinthenormalratspinalcordanddorsalrootganglia.Inresponsetoneurotomy,theirexpressionreachedahigherlevelandpersistedforashorttimethendeclinedtothenormallevelrapidly.Besides,positivestainingofFlk-1wasobservedinbothglialcellsandnervefibers,whichlocatedinthewhitematterofthespinalcord.Conclusions:VEGFcanpromotetheregenerationofperipheralnervesfromtheangleofcentralneurons,whichestablishestheexperimentalandtheoreticalfoundationforVEGFtreatingperipheralnerveinjuries.