简介:Wehavepreviouslydemonstratedtheabilityofmalariaparasitestointerferewithspecificimmuneresponses.CD4Tcellsspecifictoparasiteantigens,butnotCD4Tcellsspecifictoanirrelevantantigen,ovalbumin(OVA),aredeletedviaapoptosisduringmalariainfection.Itisofinterest,therefore,toinvestigatetheimmuneresponsesthatdevelopedfollowingvaccinationwiththe19kDacarboxylterminusofthemerozoitesurfaceprotein1(MSP119)inmicethathadpreviouslyexperiencedmalariainfection.Inthisstudy,pre-exposureofmicetoPlasmodiumyoeliielicitednativeanti-MSP119antibodyresponses,whichcouldbeboostedbyvaccinationwithrecombinantMSP119,Likewise,infectionofMSP119-primedmicewithPlasmodiumyoelii(P.yoelii)ledtoanincreaseofanti-MSP119antibodies.MSP119vaccinationofmalariapreexposedmiceorimmunizationbyinfection/cureofMSP119-primedmiceenabledthemicetosurvivechallengeinfection,withtheformergrouphavingslightlylowerparasitaemia.Thedatasuggestthatexposuretomalariainfectionprimesanaturalimmuneresponsewhichcanbeboostedbyvaccination.Thisinformationisrelevanttothedevelopmentofavaccineforuseinindividualslivinginmalaria-endemicareas.
简介:Toinvestigatethematernal-infantileinfectionwithhumanparvovirusB19,theIgGandIgMantibodiesagainsthumanparvovirusandtheB19-DNAinserumandperipheralbloodmononuclearcells(PBMC)ofpregnantwomenaswellastheserumIgMantibodyagainstB19andtheB19-DNAinserumandcordbloodnucleatedcells(CBNC)ofnewbornsweredeterminedbyELISAandnestedPCRrespectively.ItwasfoundthatthepositiverateoftheIgGantibodyagainsthumanparvovirusB19inseraof92pregnantwomenwas38.04%(35/92),andthatoftheIgMantibodyin720pregnantwomenwas9.03%(65/720).However,theIgMantibodyagainsthumanparvovirasB19wasnegativeinthecordbloodseraof95newborns.AstothehumanparvovirasB19DNA,noneof720pregnantwomenand95newbornswasprovedtobepositiveintheirsera,Nevertheless,thepositiverateoftheparvovirasB19DNAinPBMCwas3.06%(3/98)in98pregnantwomenand1.12%(1/89)inCBNCof89newborns.ItisconcludedthatthehistoryofinfectionwithhumanparvovirasB19existsincertainpregnantwomenwithasmallpercentageofpregnantwomeninfectedwithrecentoracuteinfectionsofB19virus.ThedetectionratesoftheB19viralDNAinPBMCofpregnantwomenandCBNCofnewbornswerehigherthanthoseinsera,indicatingthattheriskforverticaltransmissionisverylow.
简介:【摘 要】医院建立在“实事求是”基础上制度设计,合理性、有效性的流程,不但满足质量保证的需要,又兼顾到质量控制。医院感染监测系统利用信息技术主动发现传染病患者实时监控,提高了新冠肺炎疫情报告的及时性和疫情监测数据的准确性。