简介：

简介：

简介：

简介：TheaimofthisstudywastoexplorethemolecularbasisfortheattenuationoftheJapaneseencephalitisvirus(JEV)vaccinestrainSA14-14-2.ThevirulenceofSA14wildJapaneseencephalitisvirus(JEV)anditsseveralattenuatedviruseswastestedbyintracerebral(i.c.)orintraperitonial(i.p.)inoculationof10-12gmice.Thestabilityofneuroattenuationwastestedbyonepassageinsucklingmousebrain.TheEproteingenesofthoseviruseswereamplifiedbyPCR,sequencedandcompared.Threeattenuatedvirusstrains,SA14-14-2vaccinevirus,SA14-9-7andSA14-5-3,didnotexhibitlethalinfectionsbyi.c.ori.p.inoculationof10-12gmiceandreverttothevirulence.Theothervirusstrain,SA14-12-1-7,showednoneuroinvasivenessbyi.p.inoculationbutresidualneurovirulencebyi.c.inoculationandrevertedtohighvirulenceafteronebrainpassage.ComparisonoftheEproteingenesequencesofthefivevirusstrainsindicatedthatthereweredifferencesoftwelvenucleotidesandeightaminoacidsbetweentheparentstrainSA14andvaccinestrainSA14-14-2,ofwhichsixaminoacids(E-107,E-176,E-439,E-138,E-279,E-315)exhibitedchangescommontothoseofSA14-9-7andSA14-5-3,threesubstitutionscommontoSA14-12-1-7.TwoaminoacidsubstitutionsatthesitesE177(T→A)andE264(Q→H)areuniquetotheSA14-14-2vaccinevirus.TheresultssuggestthatthemutationsofE-107(Leu→Phe),E-176(He→Val),andE-439(Lys→Arg)maycontributefortheattenuationofneuroinvasivenessandpartiallyfortheattenuationofneurovirulence,themutationsofE-138,E-279,E-315maynotonlycriticaltotheneuroattenuationbutalsotoitsstability.

简介：【摘要】目的：分析米非司酮联合米索终止10一14周妊娠的效果及安全性。方法：研究对象选择2017年8月一2018年9月的36例采用米非司酮联合米索前列醇终止10一14周妊娠（药物组）案例作为实验组，选择同期收治的36例10一14周妊娠案例钳刮术（手术组）作为对照组。 结果：实验组效果及安全性高于对照组。结论：米非司酮联合米索前列终止10一14周妊娠安全有效，整体优势明显，值得实施应用。