An SEM study on Mullet gill filament material and methods

Theinvestigationwasperformedon4Mullets（27～49cmlongandweighing225～1500geach）ofbothsexesfromthewatersoffTianjin.Smallpiecesofthegillswereslicedandimmersionfixedinabuffermixtureof1.5%glutaraldehyde,1.5%paraformaldehydeand0.1mol/dm3phosphateforatleast24h.Afterfixationinphosphatebuffered1%O?O4at4℃for1h,thetissueswere