ComparedwiththespeciesofLeucesection,thedifficultyinplantregenerationduringtissuecultureforthespeciesofAigeirossectionisakeylimitingfactorfortheirapplicationingeneticengineeringofforesttrees.Inthisstudy,theplantgrowthregulatorscombination,copperconcentration,lightintensity,andtheselectionpressuresofkanamycinwereinvestigatedusingleavesofPopulus×euramericanacl.’Bofeng’asexplants,andastableandhighefficientregenerationsystemwasestablished.Usingthissystem,boththeshootregenerationrateandrootingratewereupto100%,andtheaveragenumberofdifferentiatedshootsineachleafexplantwasupto20,andthesurvivalrateofseedlingsreached98%.Theoptimalselectionpressureofkanamycininshootinducementforleaf-explantwas40mg·L-1,andtheoptimalselectionpressureofkanamycininrootinducementofadventitiousbudwas20mg·L-1.TheauthorsalsofoundthatdifferentiationrateofadventitiousbudsofP.×euramericanacl.’Bofeng’couldbesignificantlypromotedbyincreasedcopperconcentration,andthisisthefirstreportaboutthekeyroleofcopper（Cu）inexplantregenerationofPoplars.ThisregenerationsystemwithhighfrequencywhichwasestablishedbytheauthorswasasgoodasthatofspeciesofLeucesection,anditprovidesagoodtechnologicalplatformforgeneticengineeringofforesttreewhichusesthespeciesofAigeirossectionasmodelmaterials.
