简介:OnOctober12,2008,atrain-ingschoolfororphanswasinauguratedatNanshanggangVillage,FangshanDistrict,BeijingMunicipality.'Oursisnotanordinaryorphanage,'saidschoolocialZhangMei.'Itaimstoproducemembersofthesocialelitebylettingorphansgrowinloveandwarmthcharacteristicofwholesomefamilies.'
简介:以传统的产品出售企业的一个不同方法提供产品和服务的一个新企业概念特别在生产工业正在得到更多的注意。这篇论文调查借助于产品和服务的集成的这家新企业怎么在德国和意大利被完成。另外,它根据公司尺寸分析差别。结果包括那这类企业在他们顾客的焦点激发的许多情况中并且经常由物理产品和他们的维护组成。与德国的对比,意大利的公司经常为这类提议明确地设计他们的物理产品。从分析基于尺寸差别,当拥有物理产品时,小公司被发现为这类提议完成特定的设计。在那里别似乎是开发支持如此的提议的发展的任何确定的方法或工具,在如此的方法/工具以内,将为更多的改编处于良好的竟技状态有空间物理产品设计。
简介:AIMTotestwhetherNox1playsaroleintyphlitisinducedbySalmonellaentericaserovarTyphimurium(S.Tm)inamousemodel.METHODSEight-week-oldmalewild-type(WT)andNox1knockout(KO)C57BL6/J(B6)micewereadministeredmetronidazolewaterfor4dtomakethemsusceptibletoS.Tminfectionbytheoralroute.Themiceweregivenplainwaterandadministeredwith4differentdosesofS.Tmbyoralgavage.Themicewerefollowedforanother4d.Fromthetimeofthemetronidazoleapplication,themicewereobservedtwicedailyandweigheddaily.Theileum,cecumandcolonwereremovedforsamplingatthefourthdaypost-inoculation.PortionsofallthreetissueswerefixedforhistologyandplacedinRNAlaterformRNA/cDNApreparationandquantitativereal-timePCR.ThecontentsofthececumwererecoveredforestimationofS.TmCFU.RESULTSWefoundNox1-knockout(Nox1-KO)micewerenotmoresensitivetoS.TmcolonizationandinfectionthanWTB6mice.Thisconclusionisbasedonthefollowingobservations:(1)S.Tm-infectioninducedsimilarweightlossinNox1-KOmicecomparedtoWTmice;(2)thesameS.TmCFUwasrecoveredfromthececalcontentofNox1-KOandWTmiceregardlessoftheinoculationdose,exceptthelowestinoculationdose(2×106CFU)forwhichtheNox1-KOhadone-loglowerCFUthanWTmice;(3)thereisnodifferenceincecalpathologybetweenWTandNox1-KOgroups;and(4)therearenoS.Tminfection-inducedchangesingeneexpressionlevels(IL-1b,TNF-α,andDuox2)betweenWTandNox1-KOgroups.TheAlpigeneexpressionwasmoresuppressedbyS.TmtreatmentinWTthantheNox1-KOcecum.CONCLUSIONNox1doesnotprotectmicefromS.Tmcolonization.Nox1-KOprovidesaveryminorprotectiveeffectagainstS.Tminfection.UsingNOX1-specificinhibitorsforcolitistherapyshouldnotincreaserisksinbacterialinfection.