简介:Famousasthekingdomofbicycles,Chinesepeoplepossess420millionbicyclesintotalby1995,reachingtheleveloftheindustriallyadvancedcountriesintermsofpercapitaownership.BicyclesareamainproductinthelightindustryofChina.AccordingtoofficialsfromtheChinaBicycles
简介:Studyonbiotinylationstrategiesforcompetitiveimmunoassayofestradiol(E2)wascarriedout.Twotypesofcompetitiveenzymeimmunoassay(EIA)withBiotin-Avidinamplificationsystemwereestablishedandoptimized.TheE2-Biotinconjugatewasusedasatracerinoneassay,andbiotinylatedantibodywasusedasatracerintheother.InbothofEIAs,horseradishperoxidase-labelledAvidin(Avidin-HRP)wasusedwithaspectrometricdeterminationofenzymeactivity.Theprecision,sensitivityandspecificityweremeasuredandcompared.Theresultsshowedthatalthoughbothweresatisfactoryinspecificity,theEIAwithhapten-BiotinshowedtobesuperiortotheEIAwithbiotinylatedantibodyinsensitivityandprecision.ThelimitofdetectionofserumE2was8and50pg/mLwithE2-Biotinandbiotinylatedantibodyastracer,respectively.
简介:Inthispaper,weconsidertwocompetitiveparalleldistributionchannels(PDCs)eachofthemconsistingofamanufacturerandaretailer.IneveryPDC,themanufacturersellsdifferentiatedproductstotheretailerandtheretailerchoosestheretailprice(margin).Weexploretheeffectsofthemovesequenceonthesystemperformancenotonlyineachdistributionchannel,butalsointheinter-paralleldistributionchannelssubjecttothedeterministicdemand.Undersomeassumptions,weshowthatthePDChasthesecond-moveradvantageinthisscenario.Thesystemperformancewillbeimprovedinourmodelwhentheparameterssatisfycertainconditions.Furthermore,wediscusstheresultsandfindsomeimplications.
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简介:Sino-U.S.economicandtraderelationsaremovingintoaneraof"competitiveinterdependence".Aneconomicandtraderelationshipthatismutuallybeneficialandwin-wininessenceisfacingchallengesthatfeatureacompetitive,zero-sumgamewhichpursuesrelativegains.Mutualsuspicionisthepsychologicalrootofthezero-sumgamebetweenChinaandtheU.S.whilethecognitiverootoftheirmutualsuspicionisthesenseofuncertaintyandlackofself-confidenceaboutthefutureofitsownandtheotherside.
简介:Anewthermokineticreducedextentmethodforstudyingofthereversiblecompetitiveinhibitionofsinglesubstrateenzyme-catalyzedreactionswasproposedinthispaper.Thereactionthatarginase-catalyzedhydrolysisofL-argininetoL-ornithineandureaandtheinhibitionofthisreactionbytheproduct,L-ornithine,andexogenousL-lysinewerestudiedat37℃in40mmol·L^-1sodiumbarbiturate-HC1buffersolution(pH=9.4).MichealisconstantKmforarginineandmaximumvelocityVmofthereactionweredeterminedtobe5.14mmol·L^-1and1.13×10^-2mmol·L^+1·s^-1,respectively.TheproductinhibitionconstantKpandinhibitoryconstantK1ofL-lysineweredeterminedtobe1.18and5.6mmol.L-l,respectively.Alltheresultshavebetterrepeatabilityandself-consistencyandareinagreementwithliteraturevalues.Thisnewmethodusingmoredirectthermalinformationfromtheprocesswouldgivemorereliablekineticinformationthanthetraditionalinitialratemethod.
简介:ThediscretedynamicsforcompetitionpopulationsofLotka-VolterratypemodeledasN1(t+1)=N1(t)exp[r1(1-N1-b12N2)],N2(t+1)=N2(t)exp[r2(1-N2-b21N1)]isconsideredinthepaper.Inthecaseofnon-persistencetheattractivebehaviorofmodelhasbeendiscussed.Especially,therearetwoattractivesetswhenbij>1,andtheattractivebehaviorsaremorecomplicatedthanthatofthecorrespondingcontinuousmodel.Theattractedregionsaregiven.Weprovethatthemodelisalsopersistentinthedegeneratecaseofbij=1.Inthepersistencecaseofbij<1,theexistenceanduniquenessfortwo-periodpointsofthemodelarestudiedatr1=r2.Theconditionforthemulti-pairoftwo-periodpointsisindicatedandtheirinfluencesonpopulationdynamicalbehaviorsareshown.
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