简介：ＡＮＯＶＥＬＲＥＤＵＣＴＩＶＥＴＥＴＲＡＡＬＬＹＬＡＴＩＯＮＯＦＰＹＲＩＤＩＮＥ￥ＸｉａｎＭｏｕＺＥＮＧ；ＪｉａＱｉａｎｇ；ＣＡＩａｎｄＹｉＪｉａｎＧＵ（ＤａｌｉａｎｉｎｓｔｉｔｕｔｅｏｆＣｈｅｍｉｃａｌＰｈｙｓｉｃｓ，ＡｃａｄｅｍｉａＳｉｎｉｃａ１...

简介：锰corroles的减少的demetalation在CH2Cl2/HCl被调查(水)由把SnCl2用作减少代理人的溶剂。demetalation收益显著地取决于corrolemacrocycle的取代者，这被发现。充满电子的锰corrole比电子缺乏的更容易经历减少的demetalation。锰5,10,15-tris(苯基)的孤立的减少的demetalation收益在现在的系统的corrole是中等的(46%)。至于电子缺乏的Mn(III)5,10,15-tris(pentafluorophenyl)corrole，在HOAc鈥揌2SO4(V/V=3:1)的导致酸的demetalation与67%的孤立的收益更好。

简介：Objective:PyruvatekinasesM(PKM),includingthePKM1andPKM2isoforms,arecriticalfactorsinglucosemetabolism.PKM2promotesaerobicglycolysis,aphenomenonknownas"theWarburgeffect".ThepurposeofthisstudywastoidentifytherolesofPKM2inregulatingcellularmetabolism.Methods:TheCRISPR/Cas9systemwasusedtogeneratethePKM-knockoutcellmodeltoevaluatetheroleofPKMincellularmetabolism.LactatelevelsweremeasuredbytheVitrosLACslidemethodonanautoanalyzerandglucoselevelsweremeasuredbytheautoanalyzerAU5800.Themetabolismof13C6-glucoseor13C5-glutaminewasevaluatedbyliquidchromatography/massspectrometryanalyses.TheeffectsofPKMontumorgrowthweredetectedinvivoinatumor-bearingmousemodel.Results:WefoundthatbothPKM1andPKM2enabledaerobicglycolysis,butPKM2convertedglucosetolactatemuchmoreefficientlythanPKM1.Asaresult,PKM2reducedglucoselevelsreservedforintracellularutilization,particularlyfortheproductionofcitrate,andthusincreasedtheα-ketoglutarate/citrateratiotopromotethegenerationofglutamine-derivedacetylcoenzymeAthroughthereductivepathway.Furthermore,reductiveglutaminemetabolismfacilitatedcellproliferationunderhypoxiaconditions,whichsupportsinvivotumorgrowth.Inaddition,PKM-deletioninducedareverseWarburgeffectintumorassociatedstromalcells.Conclusions:PKM2playsacriticalroleinpromotingreductiveglutaminemetabolismandmaintainingprotonhomeostasis.ThisstudyishelpfultoincreasetheunderstandingofthephysiologicalroleofPKM2incancercells.

简介：IntroductionReductivedesulfurizationofthioamidestoaminesisoneofthemethodstoprepareaminesandisgenerallyachievedbya)Zninacid,b)sodiumoraluminumamal-gams,c)lithiumalumminiumhydride,d)RaneyNiande)electrolyticreduction.Thesemethodsarenotveryconvenienttobeoperatedandsomeneedmorecomplexinstrument.Hereisreportedthereductivedesulfurizationofthioamidestoaminesbycatalytichydrogentransferreaction(CHT).

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简介：Ashortandfacilesynthesisofaseriesof1,2-dihydroquiazolin-4(3H)-oneswasaccomplishedingoodyieldsvianovelreductivecyclizationofo-nitrobenzamideswithketonespromotedbyTiCI4/Znsystem.Thestructurewasestablishedonthebasisofelementalanalysis,IR,^1HNMRandconfirmedbyasingle-crystalX-raydiffractionanalysis.Thismethodhastheadvantagesofeasilyaccessiblestartingmaterials,convenientmanipulationandmoderatetohighyields.

简介：Amildandrapidmethodwasfoundforthereductivecleavageofthecarbon-sulfurbondinthealkylthiocyanatesbysamarium/Cp2TiC12systemtogivethecorrespondingdialkyldisulfidesinmoderatetogoodyields.

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