简介：不同于在行星的边界层的风骚乱光谱上的以前的研究，这调查集中于高高度(15km)在各种各样的天气下面的风精力光谱和骚乱光谱调节。快Fourier变换(快速傅里叶变换)被用来基于风介绍在高高度(15km)计算风精力和骚乱光谱密度雷达(WPR)大小。在在高高度的稳定的天气条件下面的骚乱光谱在2的一个频率范围以内在力量被表示吗？？

简介：obtainaninitialoverviewofgenediversityandexpressionpatterninporcinethymus,11,712ESTs(ExpressedSequenceTags)from100-day-oldporcinethymus(FTY)weresequencedand7,071cleanedESTswereusedforgeneexpressionanalysis.ClusteredbythePHRAPprogram,959contigsand3,074singletswereobtained.Blastsearchshowedthat806contigsand1,669singlets(totally5,442ESTs)hadhomologuesinGenBankand1,629ESTswerenovel.AccordingtotheGeneOntologyclassification,36.99%ESTswerecatalogedintothegeneexpressiongroup,indicatingthatalthoughthefunctionalgene(18.78%indefensegroup)ofthymusisexpressedinacertaindegree,the100-day-oldporcinethymusstillexistsinadevelopmentalstage.Comparativeanalysisshowedthatthegeneexpressionpatternofthe100-day-oldporcinethymusissimilartothatofthehumaninfantthymus.

简介：Aresidual-stressprofilealongthethicknessofanaluminumalloysheetisdeterminedbylaser-ultrasonictechnique.SurfaceacousticwavesaregeneratedbyaNd:YAGpulselaseranddetectedbyaHeterodyneinterferometeronalateralfreesurfaceofthesheet.Thedistributionofresidualstressisdeterminedbymeasuringtherelativevariationofthewavevelocitiesatdifferentlocationofthesamplealongitsthickness.Thistechniqueisvalidatedbythreedifferentresidualstressprofilesobtainedexperimentally.

简介：二维的胶化电气泳动(2-DE)由帮助矩阵的激光解吸附作用电离time-of-flight/time-of-flight团spectrometry(MS)列在后面分析习惯于hemolymphproteomic蚕介绍的charaterize，Bombyxmori。在天4(V4)并且5(V5)第五(期末考试)中间形态当幼虫在快成长的阶段时，我们在代表有30kDa的近似分子的重量(MW)的蛋白质的点发现了戏剧的变化。这些点，四30K蛋白质是高度起来调整的，暗示有B的生长和发展的一个靠近的协会。mori幼虫。为了理解分子的基础和内在的机制，在开发和变形包含了，在V5的整个hemolymph的proteome与LTQ-Orbitrap用猎枪液体层析双人脚踏车团spectrometry被分析。没有任何假发现点击，108蛋白质的一个总数被识别。这些蛋白质涉及许多细胞的功能，包括新陈代谢，发展，滋养的运输和储备，并且防卫反应。基因本体论分析证明3.4%这些蛋白质举办了滋养的水库活动，5.7%涉及对刺激的反应。小径分析表明有普通目标的22蛋白质涉及象免疫，区别，增长和变形那样的各种各样的细胞的过程。这些结果建议象在hemolymph的30K蛋白质那样的一些关键因素在B起重要作用。mori生长和开发。而且，hemolymph的多重函数可以被一个复杂生物网络操作。

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简介：蛋白质领域被保存，在相互作用起一个重要作用在之中的机能上地独立的结构联系了蛋白质。域域相互作用最近被用来预言蛋白质蛋白质相互作用(PPI)。一般来说，一双领域的相互作用概率用训练得分功能被获得。令人满意阀值，当“交往”，带那些领域的蛋白质对被考虑。在这研究，蛋白质的签名内容被利用在Saccharomycescerevisiae，Caenorhabditiselegin，和人预言PPI对现代人。蛋白质签名模式的类似被获得，PPI预言基于二进制类似得分函数被拉。结果证明由建议途径的预言的真积极的率用最大的可能性评价方法比那高是约32%，导致在与一个测试集合相比操作的接收装置下面的区域的22%增加什么时候特征(巨鸟)曲线。当包含一个或二签名的蛋白质被移开时，预言的PPI对的敏感显著地增加了。预言的PPI对是平均，11更多半预定在0.95的信心水平比随机的选择交往，并且平均，4比那些由也预言更好预定种系发生的介绍或基因表达介绍。

简介：在从南船座介绍的situ观察，与卫星相结合的漂流检索了SST，雨率被用来通过2012年8月29日从20调查上面的海洋回答到台风Bolaven。在台风Bolaven的经过以后，加深混合的层深度(MLD)，并且冷却混合的层温度(MLT)被观察。在混合的层咸度(MLS)的变化显示出因为在混合的层的导致台风的咸度变化被降水，蒸发，狂暴的混合和thermocline水的upwelling影响，增加并且减少的一个相等的数字。加深MLD并且冷却MLT显示了重要向右偏爱，而MLS被添到台风轨道的左边并且在另外的方面上增加了。铱漂流观察的集中的温度和咸度侧面使在台风的经过以后在上面的海洋看反应过程可能。冷却在近表面并且温暖在表面下被在暴风雨的开发阶段期间定位到气旋的磁道的左边的二铱漂流观察，在远处，最大值的半径相对台风中心弯屈。在混合的层的底和thermocline的顶的水咸度增加是那些二漂流观察的最明显的变化。在轨道并且在台风中心附近的右边上当台风被加强时，从海表面的重要冷却到200的深度

简介：Theprofilingmethodisthefirstmethodtoselectinmeasuringtheremotesensingreflectance.InthelightofthecharacteristicsofChina'scoastalwaters,wedevelopanewmethodtocompensatetheenvironment'seffectswiththedownwellingirradiance'sprofileandtoestimatetheunderwaterremote-sensingreflectancerrs(λ).Theresultindicatesthattherelativedeviationofrepetitiousrrs(λ)inonestationisaround10%.

简介：Theimplementationofmolecularprofilingtechnologiesinoncologydeepensourknowledgeforthemolecularlandscapesofcancerdiagnoses,identifyingaberrationsthatcouldbelinkedwithspecifictherapeuticvulnerabilities.Inparticular,thereisanincreasinglistofmolecularlytargetedanticanceragentsundergoingclinicaldevelopmentthataimtoblockspecificmolecularaberrations.Thisleadstoaparadigmshift,withanincreasinglistofspecificaberrationsdictatingthetreatmentofpatientswithcancer.Thisparadigmshiftimpactsthefieldofclinicaltrials,sincetheclassicalapproachofhavingclinico-pathologicaldiseasecharacteristicsdictatingthepatients'enrolmentinoncologytrialsshiftstowardstheimplementationofmolecularprofilingasprescreeningstep.Inordertofacilitatethesuccessfulclinicaldevelopmentofthesenewanticancerdrugswithinspecificmolecularnichesofcancerdiagnoses,therehavebeendevelopednew,innovativetrialdesignsthatcouldbeclassifiedasfollows:i)longitudinalcohortstudiesthatimplement(ornot)'nested'downstreamtrials,2)studiesthatassesstheclinicalutilityofmolecularprofiling,3)'master'protocoltrials,iv)'basket'trials,v)trialsfollowinganadaptivedesign.Inthepresentarticle,wereviewtheseinnovativestudydesigns,providingrepresentativeexamplesfromeachcategoryandwediscussthechallengesthatstillneedtobeaddressedinthiseraofnewgenerationoncologytrialsimplementingmolecularprofiling.Emphasisisputonthefieldofbreastcancerclinicaltrials.

简介：Objective:Toanalyzethechangesofgeneexpressioninphenylbutyrateinduceddifferentiationofgliomacells.Methods:Theexpressionlevelsof14000genesingliomacellsbeforeandafterinducementwithsodiumphenyl-butyratefor2hor6dayswereevaluatedbycDNAarraytechniqueandprovedbymulti-dotblotting.Results:expressionof98genesingliomacellsshowedchangesaftertheinducement.Somegenesinvolvedintranscriptionandtranslationandsomeoncogenesaredown-regulated,whilesomegeneinvolvedindifferentiationorapoptosisareup-regulated.18unknownexpressionsequencingtag(EST)changedtoo.Conclusion:Ageneexpressionprofileassociatedwithdifferentiationofgliomacellswasestablished.

简介：Wereportonthedesignandperformanceofafiberlasersystemwithadaptiveacousto-opticmacropulsecontrolforanovelphotocathodelaserdriverwith3Dellipsoidalpulseshaping.Thelasersystemincorporatesathreestagefiberamplifierwithanintegratedacousto-opticalmodulator.Adigitalelectroniccontrolsystemwithfeedbackcombinesthefunctionsofthearbitrarymicropulseselectionandmodulationresultinginmacropulseenvelopeprofiling.Asabenefit,anarrowtemporaltransparencywindowofthemodulator,comparabletoalaserpulserepetitionperiod,effectivelyimprovestemporalcontrast.Inexperiments,wedemonstratedrectangularlaserpulsetrainprofilingattheoutputofathree-cascadeYb-dopedfiberamplifier.

简介：微数组技术是的模仿的微严肃(SMG)生物反应器和DNA识别“玩的空间基因”的强大的工具在对微严肃的细胞的反应给角色调音。我们使用了这些生物工学工具由用生物反应器在正常严肃为15，50，和60d由恢复culturing跟随了的高方面比率容器为3，4，9，和10d暴露房间到SMG在人的表皮的keratinocytes上调查SMG和post-SMG恢复效果。作为结果，我们鉴别162差别表示了基因，其32是“最一致地在路线试验的时间被影响的中心基因”。十一中心基因从综合压力反应小径并且是并列地下面调整的。七个中心基因，都是金属硫蛋白MT-I和MT-IIisoforms，是并列地起来调整的。另外，HLA-G，在细胞的有免疫力的反应抑制的关键基因，被发现在恢复阶段期间显著地起来调整。总的来说，超过80%差别从更短的暴露表示了基因(/=9d)暴露，超过50d被需要恢复到更短的暴露的影响水平。数据显示那更短的SMG暴露持续时间将从微严肃效果导致更快、更完全的恢复。

简介：Inthisstudy,wepresentaconstructivealgorithmfortrainingcooperativesupportvectormachineensembles(CSVMEs).CSVMEcombinesensemblearchitecturedesignwithcooperativetrainingforindividualSVMsinensembles.Unlikemostpreviousstudiesontrainingensembles,CSVMEputsemphasisonbothaccuracyandcollaborationamongindividualSVMsinanensemble.AgroupofSVMsselectedonthebasisofrecursiveclassifiereliminationisusedinCSVME,andthenumberoftheindividualSVMsselectedtoconstructCSVMEisdeterminedby10-foldcross-validation.ThiskindofSVMEhasbeentestedontwoovariancancerdatasetspreviouslyobtainedbyproteomicmassspectrometry.BycombiningseveralindividualSVMs,theproposedmethodachievesbetterperformancethantheSVMEofallbaseSVMs.

简介：AbstractBackground:Recent studies have demonstrated that microRNAs (miRNAs) in the blood circulation can serve as promising diagnostic markers for cancers. This four-stage study aimed at finding serum miRNAs as potential biomarkers for lung adenocarcinoma (LA) diagnosis.Methods:The study was carried out between 2016 and 2017. The Exiqon miRNA qPCR panel (3 LA vs. 1 normal control [NC] pooled serum samples) was used for initial screening to acquire miRNA profiles. Thirty-five dysregulated miRNAs were further evaluated in the training (24 LA vs. 24 NCs) and testing stages (110 LA vs. 110 NCs) using quantitative real-time polymerase chain reaction assays.Results:Four serum miRNAs (miR-133a-3p, miR-584-5p, miR-10b-5p, and miR-221-3p) were significantly overexpressed in LA patients compared with NCs. The diagnostic value of the four-miRNA panel was validated by an external cohort (36 LA vs. 36 NCs). The areas under the receiver operating characteristic curve of the four-miRNA panel in the training, testing, and external validation stages were 0.734, 0.803, and 0.894 respectively. Meanwhile, the expression level of miR-221-3p was much higher in LA tumor samples than that in the adjacent normal tissues (19 LA vs. 19 NCs). The expression level of miR-10b-5p was also elevated in the serum-derived exosomes samples (18 LA vs. 18 NCs). The expression of miR-133a-3p, miR-584-5p, and miR-10b-5p was significantly elevated in LA patients with epidermal growth factor receptor mutation compared with NCs.Conclusion:The study established a four-miRNA signature in serum that could improve the diagnostic capability of LA.

简介：Objective:Livermetastasis,whichcontributessubstantiallytohighmortality,isthemostcommonrecurrentmodeofcoloncarcinoma.Thus,itisnecessarytoidentifygenesimplicatedinmetastaticcolonizationoftheliverincoloncarcinoma.Methods:WecomparedmRNAprofilingin18normalcolonmucosa(N),20primarytumors(T)and19livermetastases(M)samplesfromthedatasetGSE49355andGSE62321ofGeneExpressionOmnibus(GEO)database.Geneontology(GO)andpathwaysoftheidentifiedgeneswereanalyzed.Co-expressionnetworkandproteinproteininteraction(PPI)networkwereemployedtoidentifytheinteractionrelationship.SurvivalanalysesbasedonTheCancerGenomeAtlas(TCGA)databasewereusedtofurtherscreening.Then,thecandidategeneswerevalidatedbyourdata.Results:Weidentified22specificgenesrelatedtolivermetastasisandtheywerestronglyassociatedwithcellmigration,adhesion,proliferationandimmuneresponse.Simultaneously,theresultsshowedthatC-X-Cmotifchemokineligand14(CXCL14)mightbeafavorablepredictionfactorforsurvivalofpatientswithcoloncarcinoma.Importantly,ourvalidateddatafurthersuggestedthatlowerCXCL14representedpooreroutcomeandcontributedtometastasis.Genesetenrichmentanalysis(GSEA)showedthatCXCL14wasnegativelyrelatedtotheregulationofstemcellproliferationandepithelialtomesenchymaltransition(EMT).Conclusions:CXCL14wasidentifiedasacrucialanti-metastasisregulatorofcoloncarcinomaforthefirsttime,andmightprovidenoveltherapeuticstrategiesforcoloncarcinomapatientstoimproveprognosisandpreventmetastasis.

简介：AbstractPurpose:Blast lung injury (BLI) is the most common damage resulted from explosion-derived shock wave in military, terrorism and industrial accidents. However, the molecular mechanisms underlying BLI induced by shock wave are still unclear.Methods:In this study, a goat BLI model was established by a fuel air explosive power. The key genes involved in were identified. The goats of the experimental group were fixed on the edge of the explosion cloud, while the goats of the control group were 3 km far away from the explosive environment. After successful modeling for 24 h, all the goats were sacrificed and the lung tissue was harvested for histopathological observation and RNA sequencing. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analysis were performed to identify the main enriched biological functions of differentially expressed genes (DEGs). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the consistency of gene expression.Results:Of the sampled goat lungs, 895 genes were identified to be significantly differentially expressed, and they were involved in 52 significantly enriched GO categories. KEGG analysis revealed that DEGs were highly enriched in 26 pathways, such as cytokine-cytokine receptor interaction, antifolate resistance, arachidonic acid metabolism, amoebiasis and bile secretion, JAK-STAT, and IL-17 signaling pathway. Furthermore, 15 key DEGs involved in the biological processes of BLI were confirmed by qRT-PCR, and the results were consistent with RNA sequencing.Conclusion:Gene expression profiling provide a better understanding of the molecular mechanisms of BLI, which will help to set strategy for treating lung injury and preventing secondary lung injury induced by shock wave.

简介：在这研究，2kHzTonpilz投影机用Terfenol-D被设计并且在ATILA当模特儿。为为研究建模的目的，一个放射的头团更好展出，这被决定了在136公里直径播送当前的反应(TCR)，在回声发生在2.4kHz和118dBre的山峰价值的地方在1m的1Pa/A发生在12kHz。另外，在从头团的中心的46公里距离的门栓在2.4kHz，和115.3dBre的山峰价值提供回声在1m的1Pa/A发生在11.5kHz。这个优化图案与3公里厚度的聚氨酯被制作并且塑造。原型在海洋技术(NIOT)的国家研究所的声学的测试设备(ATF)被测试为它的在水下表演。基于结果，基本回声决心是2.18kHz和182dBre的TCR的山峰价值在1m的1Pa/A发生在14kHz。RS的最大的价值被发现是在在2.1kHz的频率的1m的190dBre1V/Pa。

简介：在四国盆的发行量在北方太平洋在副热带的旋回在自台湾东面的菲律宾海流向日本的暖流和水交换的小径起一个很重要的作用。介绍在四国盆部署的漂流的南船座被用来在盆学习发行量和水群众。轨道和从所有Argofloats导出的停车的深度速度领域在四国盆在2000m显示出逆旋风的发行量。在四国盆有inhanced旋涡活动，它在四国盆发行量模式上有大影响。温度的特征-咸度曲线显示有北方PacificOcean热带水(NPTW)，诺思太平洋副热带的模式水(NPSTMW)和在四国盆的北方PacificOcean中间的水(NPIW)。NPTW仅仅是32°N向南存在。盆的Inthe中间的部分，它是28°～31°N，133°～135°E，有一个融合区域。水集中在四国盆与水来自自台湾东面的菲律宾海流向日本的暖流混合。

简介：Insulinsecretorygranules(ISGs),agroupofdistinguishingorganellesinpancreaticβcells,areresponsibleforthestorageandsecretionofinsulintomaintainbloodglucosehomeostasis.ThemolecularmechanismsofISGbiogenesis,maturation,transportation,andexocytosisarestilllargelyunknownbecausetheproteinsinvolvedinthesedistinctstepshavenotbeenfullyidentified.Subcellularfractionationbydensitygradientcentrifugationhasbeensuccessfullyemployedtoanalyzetheproteomesofnumerousorganelles.However,useofthismethodtoelucidatetheISGproteomeislimitedbyco-fractionatedcontaminantsbecause1SGsareverydynamicandhaveabundantexchangesorcontactswithotherorganelles,suchastheGolgiapparatus,lysosomes,andendosomes.Inthisstudy,wedevelopedanewstrategyforidentifyingISGproteinsbyproteincorrelationprofiling(PCP)-basedproteomics,whichincludedISGpurificationbyOptiPrepdensitygradientcentrifugation,label-freequantitativeproteome,andidentificationofISGproteinsbycorrelatingfractionationprofilesbetweencandidatesandknownISGmarkers.Usingthisapproach,wewereabletoidentify81ISGproteins.Amongthem,TM9SF3,anine-transmembraneprotein,wasconsideredahighconfidenceISGcandidateproteinhighlightedinthePCPnetwork.FurtherbiochemicalandimmunofluorescenceassaysindicatedthatTMgSF3localizedinISGs,suggestingthatitisapotentialnewISGmarker.