简介:摘要目的分析长链非编码RNA(lncRNA)ATG16L2-211在肺腺癌组织和细胞株中的表达,研究其对肺腺癌细胞生长和迁移的影响及其分子机制。方法本研究为实验室研究。GEPIA在线数据库分析ATG16L2-211在肺腺癌组织中的表达情况。采用实时定量聚合酶链式反应检测ATG16L2-211在肺腺癌细胞株(H1650、A549、H1975、H1299)中的表达情况。将ATG16L2-211序列和阴性对照序列转入H1650细胞,分别标记为ATG16L2-211组和阴性对照组。CCK-8法检测H1650细胞活力,细胞划痕实验检测H1650细胞迁移。GEPIA在线数据库分析ATG16L2-211相关性较高的基因。实时定量聚合酶链式反应和Western blot检测ATG16L2-211相关基因的表达。结果ATG16L2-211在肺腺癌组织中表达低于正常组织(t=48.12,P<0.001)。ATG16L2-211在肺腺癌细胞株中表达均低于正常肺泡上皮细胞(P值均<0.05),H1650细胞中ATG16L2-211表达最低(F=13.79,P<0.001)。与阴性对照组比较,从2 d开始至5 d ATG16L2-211组H1650细胞增殖活力均降低(P值均<0.05)。阴性对照组和ATG16L2-211组划痕愈合率为(72.15±6.23)%和(21.54±4.08)%,ATG16L2-211组H1650细胞迁移能力降低(t=6.79,P=0.001)。肺腺癌组织中ATG16L2-211和GAS6-AS1表达呈显著正相关(r=0.60,P<0.001)。与阴性对照组比较,ATG16L2-211组H1650细胞中GAS6-AS1表达增加(t=3.37,P=0.015),葡萄糖转运蛋白1基因表达降低(t=4.33,P=0.005),转化生长因子β1信号通路蛋白表达降低。结论ATG16L2-211在肺腺癌组织及细胞株中低表达,上调ATG16L2-211能通过促进GAS6-AS1表达发挥抑制肺腺癌细胞生长和迁移的作用。
简介:and evaluating the success of a particular strategy. They have an executive function. In O’Malley and Chamot framework of learning strategies,Oxford’s taxonomy fails to make a distinction between strategies directed at learning the L2 and those directed at using it (Ellis). The last problem is that compensation strategies are considered as a direct type of learning strategies rather than one type of production strategies,cognitive and social/affective. They grounded the study of learning strategies within the information-processing model of learning developed by Anderson. Metacognitive strategies involve consciously directing one’s efforts into the learning task. These strategies are higher order executive skills that may entail planning learning
简介:Thispapercontainsadetailed,selfcontainedandmorestreamlinedproofofthel~2decouplingtheoremforhypersurfacesfromthepaperofBourgainandDemeterin2015.TheauthorshopethiswillserveasagoodwarmupforthereadersinterestedinunderstandingtheproofofVinogradov'smeanvaluetheoremfromthepaperofBourgain,DemeterandGuthin2015.
简介:NineteenL-N-2-hydroxyethylaminoacidsandL-N,N-bis(2-hydroxyethyl)aminoacidswerepreparedfromthereactionofchlorohydrinwithL-alanine,L-valine,L-leucine,L-isoleucine,L-phenylalanine,L-serine,L-thrcunine,L-glutamicacid,L-asparticacidandglycine.L-N,N,N’,N’-tetra(2-hydroxyethyl)cystinewaspreparedbythereactionofL-cystinewithchlorohydrin.
简介:Peerrevision,asupplementarystrategytoteacherrevisionofwritinginL2classroom,makespassivereceiversofteacherrevisionbecomeactiverevisers,enablingstudentstoinvolveinmoremotivatedlanguagelearning.Benefitsofpeerrevisionanditsimplicationsforteacherandstudentrolesarediscussed.Thisarticlealsotentativelyanalyzeswaysofpreparingstudentsforeffectivepeerrevision.
简介:ConsiderL2-projectionuhofuton-degreefiniteelementspaceonone-dimensionaluniformgrids.TwodifferentclassesoftheorthogonalexpansioninanelementforconstructingasuperclosetofunctionuhareproposedandthensuperconvergenceforbothuhandDuhareproved.Whennisoddandnoboundaryconditionsareprescribed,thenuhisofsuperconvergenceatn+1orderGausspointsGn+1ineachelement.Whennisevenandfunctionvaluesontheboundaryareprescribed,thenuhisofsuperconvergenceatn+1orderpointsZn+1ineachelement.Iftheotherboundaryconditionsaregiven,thentheconclusionsarevalidinallelementsthatitsdistancefromtheboundary≥ch|lnh|.Theaboveconclusionsarealsovalid.forn-dergreerectangularelementQ1(n).
简介: Enantioimericallypure1,l'-bi-2-naphthol(BINOL)1anditsderivativesareimportantchiralligandsandauxiliariesforanumberofasymmetrictransformations1suchasaldolcondensations,alkylations,Diels-Alderreactions,Michaeladditions,epoxidations,etc.Opticallypure1andtheirderivativeshavealsobeenusedextensivelyinchiralrecognition,chiralseparationandintheconstructionoffunctionalisedmaterials2.Furthermore,(R)-and(S)-BINOLarealsofundamentalstartingmaterialsforthesynthesisofalargevarietyofotherchiralbinaphthylssuchasMOP(2c),NOBIN(2d)andBINAP(2e).Asaresultthedevelopmentofefficientandeconomicmethodsforthepreparationofopticallypure1hasattractedmuchattentioninrecentyears3andnovelmethodsfortheresolutionof(±)-1continuetobedeveloped.……
简介:Objective:ToinvestigatetheexpressionofHPV16mRNAinnormalhumankeratinocytestransfectedwithpSV2-neo/16.Firsthumankeratinocyteswereculturedintheserum-freemediumM154.Second,theplasmidpSV2-neo/16wastransfectedintothehumankeratinocytesusingatransfectingreagent.Third,RT-PCRandSouthernBlottingwereusedtodetecttheexpressionofHPV16mRNAandDNAinthetransfectedkeratinocytes,respectively.Results:TheexpressionofHPV16mRNAwassuccessfullyamplifiedandan110bpwasditectedbyRT-PCR.A7.9kbfragmentwasconfirmedinthetransfectedkeratinocytesbySouthernBlotanalysis.Conclusion:HPV16mRNAandDNAweresuccessfullydetectedinthehumankeratinocytes.
简介:identification and classification of learning strategy. The problems are reviewed concerning the definition and classification of learning strategies and then the paper tentatively introduces Cohen’s approach to defining learning strategies in terms of prototypicality of features of learning strategies.,Cohen’s attempt to describe the prototypicality of strategies is a step forward concerning defining learning strategies. It might reflect the nature of learning strategies to a large extent because the answers to the questionnaire come from strategy experts. It is true that some problems still exist. For example,all-or-nothing feature but in terms of how far along a continuum a feature could possibly go before it stopped being descriptive of a strategy. This is an approach of defining learning strategies in terms of how prototypical the feature was. These features as follows are arranged in a descending order of agreement
简介:LetAbead×drealexpansivematrix.AnA-dilationParsevalframewaveletisafunctionψ∈L2(Rd),suchthattheset{|detA|n/2ψ(Ant-l):n∈Z,l∈Zd}formsaParsevalframeforL2(R~d).AmeasurablefunctionfiscalledanA-dilationParsevalframewaveletmultiplieriftheinverseFouriertransformoff■isanA-dilationParsevalframewaveletwheneverψisanA-dilationParsevalframewavelet,where■denotestheFouriertransformofψ.Inthispaper,theauthorscompletelycharacterizeallA-dilationParsevalframewaveletmultipliersforanyintegralexpansivematrixAwith|det(A)|=2.Asanapplication,thepath-connectivityofthesetofallA-dilationParsevalframewaveletswithaframeMRAinL2(Rd)isdiscussed.