简介:AIM:Toinvestigatetheantifibroticeffectofthefreeze-driedbilayeredfibrin-bindingamnioticmembraneasadrugdeliverysystemonglaucomasurgeryinrabbitmodel.Theaimofthisstudywastoprepareanovellocaldeliverysystemforthesustainedandcontrollablereleaseof5-Fu.METHODS:Twenty-fourJapanesewhiterabbitswererandomizedintothreegroups:theexperimentalgroup(oculartrabeculectomyincombinationwith5-Fuloadedfreeze-driedbilayeredfibrin-bindingamnioticmembranetransplantation),thecontrolgroup(oculartrabeculectomyincombinationwith5-Fu)andtheblankgroup(singletrabeculectomy).HEstaining,massionstainingandimmunohistochemistryforα-SMAwereperformedondays7,14,21and30followingsurgery.Theconcentrationof5-Fuinrabbitaqueoushumorwasexaminedbyhighperformanceliquidchromatography(HPLC)3daysafterthesurgery.RESULTS:Statisticaldifferenceswerenotedinintraocularpressureamonggroupsonday7,14,21and30followingsurgery.Histologyfurtherdemonstratedthattrabeculectomyincombinationwithfreeze-driedbilayeredfibrin-bindingamnioticmembraneyieldedwellwoundhealingandnoscarformationandwasbeneficialforlongtermeffect.CONCLUSION:HPLCshowedagoodslow-releaseeffectwithfreeze-driedbilayeredfibrin-bindingamnioticmembrane.
简介:摘要目的应用前胸壁、心脏结构、胸椎识别法和四腔心十字交叉偏移法,探讨胎儿心脏致命性畸形的诊断和临床应用。方法选取2006年1月—2008年12月在我院常规体检中孕胎儿(18—24周)胎儿心脏筛查,5078例应用前胸壁、心脏结构、胸椎识别法和四腔心十字交叉偏移法检查,4868例采用常规方法(对照组)进行胎儿心脏超声检查。结果用此法在孕检同时对5078例胎儿心脏结构的筛查中发现心脏致命性畸形10例,漏诊2例。诊出率83%。常规方法(对照组)发现心脏致命性畸形3例,漏诊6例。诊出率33%。结论前胸壁、心脏结构、胸椎识别法和四腔心十字交叉偏移法,可以在孕期常规检查的同时以最短时间,准确识别心脏的正常或异常结构,最大限度地筛查胎儿心脏致命性畸形,且此法思路清晰,明确,方法简单,易于掌握,准确率高,具有很强的可操作性,对于孕期胎儿心脏致命性畸形的筛查具有重大的临床和社会意义。