简介:Efficacyofacupuncturetherapyvariesinpatientswithsimilarailments.Thepresentstudywasundertakentosearchforamarkerforbetterefficacyofacupuncturetherapy.Thestudywasmadein224patientsincludingosteoarthritis141(62.94%),polyarthritis23(10.26%),Bursitis&synovitis15(6.69%)andothers45(20.08%).ABObloodgroupsweretestedforeachpatient.ItappearsthatpatientsbelongingtogroupABandBrespondedwelltoacupuncturetherapyinproportionatelymorenumber.Goodresultwasachievedin47.82%casesingroupABand46.04%casesingroupB,whereaspatientsofgroupAandOshowedgoodresultin27.65%and26.15%casesrespectively.ApparentlyitmaybeconcludedthatpatientsofAB&Bbloodgroupswouldrespondcomparativelywelltoacupuncturetherapy.
简介:NF-κBisatranscriptionfactorofeukaryote,fivemembersofwhosefamilyinmammalsandthreeindrosophila.TranscriptionfactorsoftheNF-κfamilyareactivatedinresponsetosignalsthatleadtocellgrowth,differentiation,apoptosisandotherevents.NF-κBtakespartinexpressionofnumerouscytokinesandadhesionmoleculeswhicharecriticalelementsinvolvedintheregulationofimmuneresponses.Inthisreview,wefocusonourcurrentunderstandingofNF-κBsignalpathwayanditsroleintheinnateandadaptiveimmuneresponsesinwhichthesetranscriptionfactorshaveakeyregulatoryfunction.Furthermorewereviewwhatiscurrentlyknownabouttheireffectsassociatedwithapoptosis.Cellular&MolecularImmunology.2004;1(5):343-350.
简介:客观:在在人的创伤的奔流和正常透镜的上皮的房间之间的原子factor-KB(NF-κB)的表示学习差别。方法:全部的RNAof前面的囊标本从受不了创伤的奔流做半量的RT-PCR并且在在他们之间的NF-κB的表示进行差别的分析的正常cadaveric眼睛施主和那些在显微镜下面被拿。结果:作为与在正常控制组的0.8337的平均数相比,NF-κB的表示等价物为在创伤的奔流患者的透镜的上皮的房间是0.9074,并且差别具有显著意义(t=2.447,P<0.05)因此。结论:NF-κB是可能的对必要的一种抄写因素维持正常透镜的上皮的房间的新陈代谢。在创伤的奔流患者可得到的更高的NF-κB“透镜的上皮的房间工具NF-κB具有到创伤的奔流的出现和开发的可能的关联的s。
简介:ThemolecularmechanismsforNF-κBsignalingtransductionandtranscriptionhavebeenthemostattractivesubjectsforbothbasicresearchandpharmaceuticalindustriesduetoitsimportantrolesinbothphysiologicalandpathogenesis,particularlythecloseassociationofdysregulatedNF-κBwithtumorgenesisandinflammation.SeveralnovelintracellularmoleculareventsthatregulateNF-κBactivityhavebeendescribedrecently,includingthediscoveryofanalternativesignalingpathwaythatappearsinducingaspecificsubsetgenesinvolvedinadoptiveimmuneresponse.Multi-levelandmulti-dimensionalregulationofNF-κBactivitybyphosphorylationandacetylationmodificationshaveunveiledandbecamethehottesttargetsforpotentiallytissuespecificmolecularinterventions.AnotheremergingmechanismforNF-κB-responsivegene'sregulationwhereNF-κBparticipatesthetranscriptionalregulationindependentofitscognateregulatorybindingsitewithinthetargetgene'spromoterbutfacilitatingthetransactionactivityofotherinvolvedtranscriptionfactors,thatimplicatedannoveltranscriptionalactivitiesforNF-κB.Thus,thecurrentreviewwillfocusontheserecentprogressesthathavebeenmadeonNF-κBsignalingtransductionandtranscription.Cellular&MolecularImmunology.2004;1(6):425-435.
简介:Inordertoinvestigatetheimmtmogenicityofthecontrolled-releasemicroencapsulatedhepatitisBvaccineinmice,polyethyleneglycol-poly-dl-lactide(PELA)microsphereswithentrappedHSsAgwerepreparedbydoubleemulsionW/O/Wbasedonsolventextractionmethods.BALB/cmicewereimmunizedwiththeencapsulatedvaccinebyoralfeedingorinjection.Bloodsampleswerecollectedat8^th,10^th,14^thand24^thweeks,respectively,andthelevelsofantibodyresponseweredetectedbyEI.ISA.Itwasfoundthatthescanningelectronmicroscopyshowedthepreparedmicrosphereshadsmoothandsphericalsurface,suitableforvaccinedelivery.Twogroupsofmiceorallyfedwiththeencapsulatedorconventionalrecombinantvaccines,respectively,theresereshowednoobviousdifferenceintheIgGlevels.At14^thweek,thegroupinjectedwithasingledoseofencapsulatedvaccinehadasimilarlevelofIgGresponsetothegroupinjectedwithtwodosesoftherecombinationvaccine.At24^thweek,theIgGlevelsofthegroupinjectedwithtwodosesofencapsulatedvaccinewerehigherthanthoseofthegroupinjectedwithtwodosesoftherecombinationvaccine.ItconcludesthatControlled-releasemicroencapsulatedhepatitisBvaccinepossessesthefeatureofslowlyreleasinginv/voandlongtimesimmtmogenicity.
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简介:目的构建Wilson病基因ATP7B的重组腺病毒载体.方法分别以BamHⅠ+SalⅠ双酶切pcDNA3.0/ATP7B和pDC315,将ATP7BcDNA目的基因片段和线性化的pDC315连接,定向克隆构建pDC315/ATP7B,以PCR和酶切的方法鉴定.pDC315/ATP7B与腺病毒骨架共转染293细胞构建Ad-ATP7B,PCR进行鉴定.结果经PCR和酶切鉴定证实pDC315/ATP7B构建成功.pDC315/ATP7B与腺病毒骨架共转染293细胞后见明显的毒斑,说明二者在293细胞中同源重组并包装成功.经PCR证实重组腺病毒Ad-ATP7B构建已完成.结论本实验成功构建了ATP7B外源目的基因序列完全正确的重组腺病毒载体Ad-ATP7B,为下一步采用ATP7B基因重组腺病毒载体对Wilson病进行基因治疗打下了基础.
简介:肝炎B病毒(HBV)感染与传染virions和非传染的空表面抗原粒子的版本首先在hepatocytes发生在肝进血液。HBV复制是non-cytopathic。短暂感染运用几个月,和长期的感染的一堂功课经常是终生的。长期的感染能与肝硬化和hepatocellular癌导致肝失败。抵销anti-HBs抗体由在感染的主人包含感染的传播并且便于病毒的粒子的移动和破坏从HBV感染在恢复起一个关键作用,这通常被接受。然而,对病毒的抗原的T房间反应开始的有免疫力的反应在HBVinfection.The为病毒的清理和疾病致病也是重要的病毒的蛋白质,HBsAg,微粒HBcAg,和nonparticulateHBeAg的三种结构的形式,可以优先地得到不同Th房间子集。在不同HBV感染地位的anti-HBs,anti-HBc,和anti-HBe的不同IgG亚纲侧面被揭示。而且,在长期的搬运人的不同IgG亚纲侧面没随着不同中高音和著名计算机生产厂商层次变化并且可以反映刺激抗原之间的差别,有免疫力的反应,并且病毒的疾病的阶段并且在人的HBV感染的高风险为个人为疫苗和预防处理的使用提供基础。这评论阐明在短暂、坚持的感染期间导致的有免疫力的回答的详细理解,和在有HBV感染的病人的免疫疗法和immunodiagnosis的发展,和减少肝的可能的工具损坏。