简介:Inthisstudy,theentiremitochondrialDNA(mtDNA)controlregion(CR)ofPholisfangiwasamplifiedviapolymerasechainreactionfollowedbydirectsequencing.ThelengthofthemtDNACRconsensussequenceofP.fangiwas853bpinlength.Inaccordancewiththerecognitionsitesaswerepreviouslyreportedinfishspecies,themtDNACRsequenceofP.fangicanbedividedinto3domains,i.e.,theextendedterminalassociatedsequence(ETAS),thecentralconservedsequenceblock(CSB),andtheCSBdomain.Inaddition,thefollowingstructureswereidentifiedinthemtDNACRsequenceofP.fangi:2ETASsintheETASdomain(TASandcTAS),6CSBsinthecentralCSBdomain(CSB-FtoCSB-A),and3CSBsintheCSBdomain(CSB-1toCSB-3).ThesedemonstratedthatthestructureofthemtDNACRofP.fangiwassubstantiallydifferentfromthoseofmostotherfishspecies.ThemtDNACRsequenceofP.fangicontainedoneconservedregionfrom656bpto815bp.Similartomostotherfishspecies,P.fangihasnotandemrepeatsequencesinitsmtDNACRsequence.PhylogeneticanalysisbasedonthecompletemtDNACRsequencesshowedthattherewerenogeneticdifferenceswithinP.fangipopulationsofthesamegeographicaloriginandbetweenP.fangipopulationsofdifferentgeographicalorigins.
简介:Mercury(Hg)isoneofthecommonlyencounteredheavymetals,whichiswidespreadininshoresedimentsofChina.InordertoinvestigatethetoxicityofHgonmarineinvertebrates,westudiedtheeffectsofthedivalentmercuricion(Hg2+)(attwofinalconcentrationsof0.0025and0.0050mgL-1,preparedwithHgCl2)onmetallothionein(MT)content,DNAintegrity(DNAstrandbreaks)andcatalase(CAT)inthegillsandhepatopancreas,antioxidantenzymeactivitiesofsuperoxidedismutase(SOD),catalase(CAT)andglutathioneperoxidase(GPx),inthehemolymph,gillsandhepatopancreasoftheportunidcrabCharybdisjaponicaforanexperimentperiodupto15d.TheresultsindicatedthatMTwassignificantlyinducedafter3d,withapositivecorrelationwithHg2+doseandtimeinthehepatopancreasandanegativecorrelationwithHg2+doseandtimeinthegills.WhileCATinthehemolymphwasnotdetected,itincreasedinthehepatopancreasduringtheentireexperiment;SODandGPxinthethreetissueswerestimulatedafter12h,bothattainedpeakvalueandthenreducedduringtheexperimentalperiod.Meanwhile,DNAstrandbreakswereallinducedsignificantlyafter12h.TheseresultssuggestedthedetoxificationstrategiesagainstHg2+inthreetissuesofC.japonica.
简介:Aciladivaricata(Hinds,1843)andA.mirabilis(AdamsandReeve,1850)arecommonbenthicbivalvesinChina.Anumberofresearchershaveproposedthatthelatterspeciesisajuniorsynonymoftheformerspecies.Becauseofmorphologicalsimilarities,itisdifficulttodistinguishthesetwospeciesbasedonvisualexaminationonly.Forbetterunderstandingoftheirtaxonomy,themitochondrialCOIgenefragmentsoffiveindividualsofA.divaricatafromtheEastChinaSeaandsixindividualsofA.mirabilisfromtheYellowSeaweresequencedinthisstudy.ThephylogeneticrelationshipsoftheobtainedCOIsequences,togetherwithnineteensequencesofthreespeciesofthegenusNucula,wereanalyzed.Thepairwiseintra-andinter-specificdistancesfortheCOIsequencesrangedfrom0.002to0.017andfrom0.128to0.134,respectively,andnooverlapwasfound.Phylogenetically,A.divaricataandA.mirabilisformdistinctcladesandclusterintoasistertoallotherNuculaspecies.TheresultsindicatedthatA.divaricataandA.mirabilisaretwodistinctspecies.Thedifferencesinthemorphologyanddistributionbetweenthetwospecieswerebrieflydiscussed.
简介:Inthispaper,wetooktheleadinstudyingonspecificityofthemicrosatelliteDNAlociandapplicabilityofmicrosatelliteDNAprimersinprotozoa.Inordertostudycharactersofmicrosatellitesinfree-livingprotozoa,eightmicrosatellitelociprimersdevelopedfromTrypanosomacruzi(MCLE01,SCLE10,MCLE08,SCLE11,MCLF10,MCLG10,MCL03,MCL05)wereemployedtoamplifymicrosatelliteinfourfree-livingprotozoa,includingBododesignis,EuglenagracilisFACHB848,ParameciumbruziseandTetrahymenathermophilaBF1.IntheamplificationsystemsofP.bruzise,fourloci(SCLE10,SCLE11,MCLF10,MCL03)wereamplifiedsuccessfully,andfouramplificationfragmentswereinpropersize.IngenomeofE.gracilisFACHB848,fiveofeightprimersbroughtfiveclearamplificationbands.InB.designis,three(No.4,5and7)ofeightlociproducedclearandsharpproductswithoutstutterbands,whereasnobandsappearedinT.thermophilaBF1.Further,eight300-500bpamplificationfragmentswereclonedandsequenced.Nevertheless,allsequencedproductsdidnotcontaincorrespondingmicrosatellitesequence,althoughBodoisinthesameorderandhasthenearestphylogeneticrelationwithTrypanosomaamongthesefourspecies.Thus,themicrosatelliteDNAprimerscannotbeappliedamongorderormorefartaxa,andthespecificityofmicrosatelliteDNAisveryhighinprotozoa.TheresultsofthisstudywillcontributetoourunderstandingofmicrosatelliteDNAinprotozoa.
简介:Inapreliminaryinvestigation,RandomAmplifiedPolymorphicDNA(RAPD)analysisandpartialmitochondrialND2genesequencingwereconductedtostudythegeneticvariationoftheIndianmackerel,Rastrelligerkanagurtaalonga450kmstretchofitsdistributiononthewestcoastofPeninsularMalaysia.Atotalof53individualsfrom6populationswereanalyzedusing4RAPDprimersandasub-sampleof15individualswaschosenforsequencingofpartialND2gene.Comparisonbetweenthe2markersrevealedgeneticstructuringintheRAPDresultsbutgenetichomogeneityforND2gene.Basedontheformertheremaybeatleast2geneticallydifferentiatedgroupsofRastrelligerkanagurtaalongthisstretch.
简介:Saccharina是最重要的无热水设备生活水兵褐之一海藻的类。在这研究,我们分析了S的transcriptome。装饰用的梨树,它属于1000植物(OneKP)工程,由使用定序技术的下一代的高产量的DNA。未加工的数据的大约5.16GB被产生,并且有454bp的平均长度的65536脚手架与肥皂denovo汇编方法被装配。总共,19040unigenes被强风识别;25734脚手架被聚类进37基因本体论功能的组;6760脚手架被分类进25个轮牙范畴,以及被分到306条KEGG小径的2665脚手架。unigenes的多数比另外的cyanobacteria,海洋的硅藻,和植物包括棕色的水藻和硅藻展出了更多的类似到水藻。Saccharina装饰用的梨树有突出的能力积累象Br那样的卤素,我从海水经由halogenation处理。我们在S获得了42不同钒依赖者haloperoxidases(vHPO)。装饰用的梨树transcriptome数据,包括钒依赖者iodoperoxidase(vIPO)的5个片断和钒依赖者bromoperoxidase(vBPO)的37个片断。识别fulllengthS的复杂分析。装饰用的梨树vBPO1和S。装饰用的梨树vBPO2在在海洋的海藻的vBPOs和vIPOs之间的棕色的水藻和强壮的关系的种类之中揭示了vBPO的重要性。这研究将提高我们生物特征的理解和S的经济价值。装饰用的梨树种类。
简介:摘要:近年来,工程建设的快速发展对建筑路基以及路面的基层施工处理提出了更高的技术要求。既要严格规范城市路基施工路面管理施工,提高路基施工管理技术水平,又必须高度重视加强路基施工路面的质量检测。大量资料表明,路基稳定性差是引起路面病害的直接原因。在外荷载作用下,强度不足会导致严重变形。中国对基础设施建设的投资正在逐步增加。在道路建设中,它是摆在各种基础设施建设面前的。在道路基础工程施工中,路基工程施工管理质量直接就影响道路交通质量,是道路施工中必须严格控制的一项关键施工技术。在普通路基基础施工中,要认真做好普通路基施工试验现场检测取样工作,通过现场路基取样试验检测,确保普通路基结构承载力能够满足基本设计施工要求,从而有效率地保证普通路基基础施工过程质量,避免了因路基结构失稳松动造成普通道路严重损坏。
简介:摘要:近年来由于建筑监理规范性很强,建筑工程施工活动中必须定期进行检验,检查结论的准确性与科学性决定着建筑监理的服务质量。工程质量必须采用物理学的方式进行全面的检验,具备合法性、公正性、真实性的特征,才能为施工做出依据。文章首次就建筑测量的性质展开研究,并就五种常用的建筑工程建筑方式展开了深层次的研究。