学科分类
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6 个结果
  • 简介:为了在萌芽能力(LTG)上调查低温度,有198根线的一张doublehaploid米饭(DH)人口源于与indicalineZhenshan97B混合的F_1的花药培养,长期的装饰用的梨树线AAV002863被用来构造连锁图with140SSR标记。在Zhenshan97B和AAV002863的萌芽率是79.7%和30.1%,当时inDH人口它在6天以后在15°C从0~100%。控制低温度germinabilitywere的量的特点loci(QTL)在染色体上识别了3和10。归因于QLTG-3和QLTG-10的观察phenotypic的百分比分别地是12.6%和12.9%。从Zhenshan97B的等位基因在qLTG-3区域增加了LTG,当时从在qLTG-10的AAV002863increasedtheLTG的等位基因区域。一个个epistatic相互作用在染色体上在loci之间被检测3和10。染色体10上的QTL主要效果也涉及epistaticinteraction。

  • 标签: 水稻 低温条件 发芽率 基因 QTL
  • 简介:到低氮应力的抄写因素基因的反应被学习为在米饭改进吸收和氮化肥的利用效率提供分子的基础。agilent米饭染色体数组被用来在低氮应力下面与不同叶绿素内容在二个米饭变化(SN196和Toyonishhiki)学习抄写因素基因的改变的表示。结果显示出那53抄写因素基因的一个总数(35下面调整并且在抄写的18起来调整的基因铺平)在超级绿色的米饭SN196和27抄写因素基因的旗帜叶子(21下面调整并且在抄写的6起来调整的基因铺平)在Toyonishiki的旗帜叶子受影响低氮应力。在那些氮应答的基因之中,在SN196的48抄写因素基因并且22在Toyonishiki是变化特定的。有因素基因回答了到在SN196和Toyonishiki之间的低氮应力的重叠抄写,与1起来调整并且4在抄写水平下面调整。低氮的分布染色体上的应答的基因在二个米饭变化是不同的。

  • 标签: 转录因子基因 低氮胁迫 水稻品种 剑叶 转录水平 叶绿素含量
  • 简介:有低glutelin内容的瑞斯作为为肾失败影响的病人的功能的食物合适。在米饭的低glutelin内容基因Lgc1有在二高度类似的glutelin基因GluB4和GluB5之间的3.5-kb删除,它在染色体2的短手臂上定位。在低glutelin内容米饭改进选择效率繁殖,指定为InDel-Lgc1-1和InDel-Lgc1-2的二个分子的标记被开发检测低glutelin内容基因Lgc1。双PCR察觉显示二个标记的联合使用能容易把Lgc1的遗传型与不同米饭变化区分开来。作为一种简单、便宜的技术,因此,分子的标记能广泛地被用来与Lgc1基因识别不同变化并且在低glutelin内容米饭的帮助标记的选择适用。

  • 标签: 蛋白基因 水稻育种 分子标记 蛋白含量 ORYZA PCR检测
  • 简介:ThaijasminericeKDML105isconsumedaroundtheworld.BKOS,PKOSandTKOSarenewcultivarsproducedfromlow-energyionbeaminductioninKDML105.ThepurposeofthisstudyistocomparethemorphologyandanatomybetweenKDML105andthethreenewcultivars.Seedsofthefourcultivarsweregerminatedandgrowninpotsuntilfloweringphase.Theplants'organswereobservedandthelengthsofculms,ligules,leavesandpaniclesweremeasured.Leafsurfaceareawascalculatedandnumbersofroots,spikeletsandtillerswerecounted.BKOSandPKOShadsignificantlyshorterculmsthanKDML105andTKOS.ThelargestleafareawasfoundinKDML105followedbyTKOS,BKOSandPKOS,respectively.NumbersofrootsandtillersinBKOSandTKOSweresignificantlyfewerthanthoseinKDML105andPKOS.ThenumberofspikeletsperplantinBKOSwasthelowestamongallcultivars.Foranatomicalcomparison,crosssectionsofculmsandrootswereobserved.Allplantshadasimilararrangementoftissues,butthenumberandsizeofcellsweredifferent.Furthermore,longitudinalsectionsofculmsshowedthatthelengthsofepidermalandparenchymacellsweredirectlyrelatedwiththelengthoftheculm.Tocomparetheleaves,bothstomataandepidermalcellswerecountedandthelengthsoftheguardcellsweremeasured.ThelengthsofguardcellsofBKOSandPKOSwereshorter,butthestomataldensityandthestomatalindexweresignificantlygreaterthanthoseofKDML105.ForTKOS,thoughthelengthofguardcellswasshorterthanthatinKDML105,thedifferencewasnotsignificant.However,thestomataldensityandstomatalindexweresignificantlyhigherthanthoseinKDML105.

  • 标签: 低能离子束 解剖 形态 诱导 突变体 维和行动
  • 简介:Nearisogeniclinescarryinglarge-effectQTL(qtl12.1),whichhasaconsistentinfluenceongrainyieldunderuplanddroughtstressconditionsinawiderangeofenvironments,wereevaluatedunderwaterstressinthefields.Thelinewhichgavehigheryieldunderdroughtwascrossedwithalocaleliteline,PMK3,andforwardedtoF2:3generation.SignificantvariationwasfoundamongtheF2:3linesforagronomictraitsunderwaterstressinthefields.Lowtohighbroadsenseheritability(H)forinvestigatedtraitswasalsofound.Waterstressindicatorssuchasleafrollingandleafdryingwerenegativelycorrelatedwithplantheight,biomassandgrainyieldunderstress.Bulkedsegregantanalysis(BSA)wasperformedwiththemarkersinthevicinityofqtl12.1,andRM27933wasfoundtobesegregatedperfectlywellinindividualcomponentsofdroughtresistantanddroughtsusceptiblebulkswhichwerebulkedbasedonyieldunderwaterstressamongF2:3lines.Hence,thissimpleandbreederfriendlymarker,RM27933,maybeusefulasapotentiallyvaluablecandidatemarkerforthetransferoftheQTLqtl12.1intheregionalbreedingprogram.BioinformaticanalysisoftheDNAsequenceoftheqtl12.1regionwasalsodonetoidentifyandanalyzepositionalcandidategenesassociatedwiththisQTLandtoascertaintheputativemolecularbasisofqtl12.1.

  • 标签: 水分胁迫条件 粮食产量 QTL 转育后代 评估 群分析