简介:Inviewoftheanti-HIVactivitiesofdelavirdineandresveratrol,anovelanti-AIDsdrugResdelasu,1-{3-[(1-methyl-ethyl)amino]-2-pyridinyl}-4-{[5-(4-{5[(1E)-2-(hydroxyphenyl)ethenyl]-1,3-benzenedioyl-carbonylbu-tanoyl}amino)-1H-indol-2-yl]carbonyl}-piperazinewassynthesizedbylinkingthedelavirdinederivativeandresveratrolwithsuccinicanhydrideastheconjugatedcompoundviatwodifferentroutes.Itsstructurewasconfirmedbymeansof1HNMR,13CNMR,IR,MS,andelementalanalysis.TherouteofesterificationfollowedbyamidationforsynthesizingResdelasuwassimplerandgaveahigheryield(46%)thanthatofamidationfollowedbyesterification.ItwasprovedthatthemethodwasefficientandconvenienttoprepareResdelasu.TheresultsofHIV-1ReverseTranscriptaseAssayandquantitativetitrationofHIV-1viaMAGIassayshowedthatanovelanti-AIDsdrug,Resdelasu,hadbeensynthesized.
简介:多保护是在安全管理的一个原则,并且反爆炸的使用和耐火的代理人是它的关键部分。在这份报纸,反爆炸的领域里的nano氧化物粒子的性质并且耐火被分析。纳米粒子的沉积速度是计算的。CH4作为一个例子被拿,并且它起始的阶段的燃烧机制被结束。反应系统的分子的精力上的15氧化物的效果与程序Hyperchem6被计算,并且在激进分子和氧化物之间的可能的接触方式被得到。氧化物例如ZrO2,能与激进分子结合,这被结束,并且因此阻止燃烧反应。nano-ZrO2暴发地产生了喷雾器,这被发现比在用作反爆炸和耐火的代理人的另外的对应物优异,因此,将在安全管理起一个日益重要的作用。
简介:HeteroatomM-ZSM-12zeolites(M=B,Al,Ge,Ga,Fe)arehydrothermallysynthesizedanditisprovedthattheheteroatomMisinvolvedintheframeworkofsynthesizedmolecularsievesbymeansofXRD,IRspectra.TheresultsofadsorptionanddiffusionexperimentsindicatethattheheteroatomMmodifiestheporevolume,specificsurfacearea,andthechannelofZSM-12molecularsieves.
简介:EachofKeggintypeheteropolytungstosilicicandheteropolytungstogermanicacidsreactedwith8-quinolinol,p-aminodimethylanilineandpyridine,respectively,yieldingsixchargetransferheteropolycomplexes(CTHPCs),whichwerepurifiedandcharacterizedbyelementalanalysis,IR,UVand183WNMR.Theanti-HIV-1activitiesofCTHPCswereevaluatedbyELISAofHIV-P24antigen.ThetoxicityagainstMT-4cellswasmeasuredbyMTT.Theresultsshowthatmedianinhibitoryconcentration(IC50)foreachCTHPCtoinhibitHIV-P24antigenincellculturewaslowerthan5μg/mL,whilemediantoxicityconcentration(IC50)againstMT-4cellswashigherthan268μg/mL.Thefollowingmechanismsmightbeconsideredfortheiranti-HIV1activity,namely,(1)inhibitingthepenetrationofHIV-1virusintotargetcellsforitcanblockadeCD4receptorofMT-4cellsand(2)inhibitionofsyncytiumformation.
简介:Theactivesiteof3CLproteinase(3CL^por)forcoronaviruswasidentifiedbycomparingthecrystalstructuresofhumanandporcinecoronavirus.Theinhibitorofthemainproteinofrhinovirus(Ag7088)couldbindwith3CL^proofhumancoronavirus,thenitwasselectedasthereferenceformoleculardockinganddatabasescreening.Theligandsfromtwodatabaseswereusedtosearchpotentialleadstructureswithmoleculardocking.SeveralstructuresfromnaturalproductsandACD-SCdatabaseswerefoundtohavelowerbindingfreeenergywith3CL^prothanthatofAg7088.ThesestructureshavesimilarhydrophobicitytoAg7088.Theyhavecomplementaryelectrostaticpotentialandhydrogenbondaeceptoranddonorwith3CL^pro,showingthatthestrategyofanti-SARSdrugdesignbasedonmoleculardockinganddatabasescreeningisfeasible.
简介:CardiactroponinI(cTnI)wasseparatedandpurifiedfromhumanleftventriculartissuebyaffinitychromatographicmethodandusedtoimmunizeBalb/cmicebyintraperitonealinjectionandfourhybridomacelllines,whichsecretedmonoclonalantibody(mAb)againsthumancTnI,wereobtainedbycellfusion,identificationandcloningtwice.ThreemAbs(9F5,2F11,8C12)wereproducedfromtheascitesofBalb/cmiceinjectedintraperitoneallythehybridomacellsandcharacterizedbymeansofasurfaceplasmonresonance(SPR)biosensor.AnoptimalandspecificsensingmembranefortroponinIwaspreparedwithstaphylococcalproteinA(SPA)astheintermediatelayerandmAbagainsthumancTnIasthecaptureantibody.Onthebasisofthesensingmembrane,twomodesofoperationoftheSPRbiosensorweredeveloped,i.e.,adirectdetectionofantigen-antibodyaffinityandasandwichassay.Inthesandwichassaydetectionmode,themAbscompetitionwasmeasuredbymonitoringwhetherthesecondaryantibodyhadbeenattachedtothecTnIalreadycapturedbythefirstantibodyonthesensorsurface.TheSPRbiosensorwasshowntobeabletodirectlydetecttheantigen-antibodyaffinityandtheorderoftheaffinitywasfoundtobe9F5>2F11>8C12.Inthesandwichdetectionmode,itwasfoundthatthedifferentepitopesonthecTnImoleculeswererecognizedbythethreemAbsrespectively,buttheasymmetricalcompetitionwasshownbetween2F11and8C12andnocompetitionwasfoundbetween9F5and2F11or8c12.Basedontheseresults,adoublemonoclonalsandwichimmunoassayforcTnIwasdevelopedbyusingtheoptimalantibodypairof9F5and2F11andtheSPRbiosensorwithSPAsubstratemembrane,whichshowedanexcellentsensitivityof0.8μg/LforboththebufferandtheserumsamplescomparedwiththedirectdetectionofcTnIforthebufferwiththelowestdetectionlimitof4μg/LandconventionalELISAwiththesensitivityof1.9μg/L.
简介:Aseriesofnewflavanonederivativesoffarrerolwasdesignedandsynthesizedasapotentinhibitorofvascularsmoothmusclecells(VSMCs)vegetationaccordingtoaconvenientmethod.Thestructuresofallthesynthesizedcompoundswereconfirmedby1HNMR,13CNMRandEIHR-MS.ThebiologicalactivitiesofthesecompoundsagainstVSMCsinvitrowereevaluated.Theassayresultsindicatethattwocompounds,5,7-dihydroxy-6,8-dimethyl-2-(2-nitrophenyl)chroman-4-one(7f)and2,3-dibromo-4,5-dihydroxydiphenylmethanone(7j)exhibitedhighactivityagainstVSMCsinvitrowithIC50valuesof9.9and6.7μmol/L,respectively,andthepreliminarystructure-activityrelationship(SAR)wasdescribed.
简介:Anewcerebroside,1-O-(β-D-glucopyranosyloxy)-(2S,3R,4E,8Z)-2-[(2′R)-2’-hydroxylignoceranoylamino]-4,8-tetradecene-3-diolwasisolatedfromthe60%EtOHextractoftraditionalChinesemedicalplantCyperusrotundusL.Itsstructurewasdeterminedonthebasisofspectroscopicdata.Thisnewcompoundshowedanti-proliferationeffectonvascularsmoothmusclecells(VSMCs).
简介:Bytheadditionalreactionofbinuclearcompounds(Me4N)2M2S4(TDT)2(M=Mo,W;H2TDT=H2CH3C6H3S2)withmononuclearcuprouscomplex,twonewM-Cu-SclustersMo2Cu2S4(TDT)2-(PPh3)2·CH3CH2OH(1)andW2Cu2S4(TDT)2(PPh3)2·0.5CH3CH2OH-0.5H2O(2)havebeenpreparedandcharacterizedbyIR,UV-Vis,31PNMRspectroscopy,cyclicvoltammetryandsinglecrystalX-raystructureanalysis.BothcompoundscrystallizeinspacegroupP21/nwithlatticeparametersa=1.0956(3),b=2.2072(3),c=2.4340(3)nm,β=100.36(2)°,V=5.790(3)nm3andZ=4for1anda=1.0965(9),b=2.2135(3),c=2.4317(4)nm,β=99.63(8)°,V=5.819(8)nm3andZ=4for2.Bothmolecularstructurescontainacubane-likeclustercore[M2Cu2S4](M=Mo,W)andtheirskeletonsarealmostthesameexceptforMatoms.Thesyntheses,structuresandspectrumcharacterizationsofthesetwoclustersarediscussed.Thethird-ordernonlinearoptical(NLO)propertyofthetwoclusterswasstudiedbythetechniqueofforwarddegeneratefour
简介:Anovelaciddyeacidwassynthesizedbythecondensationreactionbetweenbromamineacidand2-amino-6-nitrobenzothiazoleanditsanti-UVprotectionpropertieswereevaluated.Theresultsshowedthatsilkdyedwiththisdyehadverygoodultravioletradiationprotectioncapabilityandexcellentdyeingperformance.TheUV-absorptionmechanismofthisdyewasalsodiscussed.