简介:Fifty-sevenstations(48gridstationsandninestratifiedstations)weresampledacrossthestudyregion(67.000°–88.394°N,152.500°–178.643°W)duringthe4thChineseNationalArcticResearchExpedition(CHINARE4)fromJulytoAugust2010bytheicebreakerR/VXuelong.Atotalof24speciesofHydromedusaewereidentifiedfrom130zooplanktonsamples,ofwhichsevenspeciesbelongedtoAutomedusa,eightspeciestoAnthomedusae,fourspeciestoLeptomedudae,andthreespeciestoSiphonophora.CatablemamulticirratumKishinouye,1910,BougainvilliabitentaculataUchida,1925,andEuphysajaponica(Maas,1909)wererecordedforthefirsttimeintheArcticsea.Inthepresentpaper,18speciesofHydromedusaeweredescribedandillustrated,ofwhichthreespeciesweredescribedforthefirsttimeintheArcticsea,and15speciesweredescribedforthefirsttimeinChina.
简介:Thegrowth,biochemicalcontentandbioaccumulationquantityof2,2’,4,4’-tetrabromodiphenylether(BDE-47)inSkeletonemacostatumwerestudiedunderdifferentN:Pratios(1,4,16,64and128).AllcellularbiochemicalcontentsofS.costatumpresenteddecreasingtrendovercultivationtime.Atearlystageofcultivation,thecellularprotein,carbohydrateandlipidinS.costatumpresentedhighervaluesintreatmentsofN:P=4and16.However,theywerelowerinthesetreatmentsatthelatestage,buthigherintreatmentsN:P=1and128.Similarly,BDE-47levelspercellofS.costatumwerehigherintreatmentsofN:P=4and16atearlystageofcultivation,whichwere3.8and3.7ng(106cells)-1,respectively.Atthemiddlestageofcultivation,theBDE-47levelperS.costatumcelllowered;anditfurtherreducedinthetreatmentsofN:P=4and16atthelatestagewiththevalues0.6and0.5ng(106cells)-1,respectively.However,itroseinN:P=128,reachingupto2.3ng(106cells)-1.ComparedwithBDE-47percell,BDE-47peralgalvolumeunderdifferentN:Pratiosdidnotpresentobviousdifference.ThequantityBDE-47accumulatedpercellofS.costatumwaspositivelycorrelatedwithprotein,carbohydrateandlipidpercell;meanwhile,theBDE-47pervolumehadapositivecorrelationwithbiochemicalcontentpervolume.ThevariationofbioaccumulationabilityofBDE-47inS.costatumcanbeexplainedbybiochemicalchangesduetoN:Pratios.
简介:Acomprehensivestrengthmonitoringsystemusedonafixedjacketplatformispresentedinthispaper.Thelong-termmonitoringofW-11-4Aplatformachieved.Structuralresponses(strainandacceleration)atselectedlocations,aswellasassociatedenvironmentalparameters,havebeenobtained.Theemphasisofthepaperisplacedonthesystemdesign,andtheinstrumentationandoperationmethodologyemployedinthemonitoringofthestructuralresponses.Theperformanceofthesystemandthecharacteristicresultsobtainedduringits13-monthoperationarealsosummarized.
简介:Thispaperpresentsthedetaildesignconditions,designcriteriaandmethodsaswellasdesignconclusionsoftheprocess,structureandcorrosionoftheproductionflowlinesandhighpressurewaterinjectionlinesinBZ34-2/4oilfieldinBohaiBay.AtpresentthisdesignrepresentsthedesigncapabilityandlevelinthefieldofthesubmarinepipelineengineeringofChina.
简介:Onthebasisofthetemperatureobservationsduring1961-2000inChina,sevencoupledgeneralcirculationmodels'(GCMs)extremetemperatureproductsareevaluatedsuppliedbytheIntergovernmentalPanelonClimateChange's4thAssessmentReport(IPCC-AR4).Theextremetemperatureindicesinusearefrostdays(FD),growingseasonlength(GSL),extremetemperaturerange(ETR),warmnights(TN90),andheatwavedurationindex(HWDI).Resultsindicatethatallthesevenmodelsarecapableofsimulatingspatialandtemporalvariationsintemperaturecharacteristics,andtheirensembleactsmorereliablethananysingleone.Amongthesevenmodels,GFDL-CM2.0andMIROC3.2performancesaremuchbetter.Besides,mostofthemodelsareabletopresentlineartrendsofthesamepositive/negativesignsastheobservationsbutforweakerintensities.Thesimulationeffectsaredifferentonanationwidebasis,with110°Nasthedivision,east(west)ofwhichtheeffectsarebetter(worse)andthepoorerovertheQinghai-TibetanPlateauinChina.Thepredictionsforthe21stcenturyonemissionsscenariosshowthatexceptdecreasesintheFDandETR,otherindicesdisplaysignificantincreasingtrend,especiallyfortheindicesofHWDIandTN90,whichrepresentthenotableextremeclimate.Thisindicatesthatthetemperature-relatedclimateismovingtowardstheextreme.Inthelate21stcentury,theGSLandTN90(HWDI)increasemostnotablyinsouthwestChina(theQinghai-TibetanPlateau),andtheFDdecreasemostremarkablyintheQinghai-TibetanPlateau,northwestandnortheastofChina.ApartfromSouthChina,theyearlychangerangeoftheextremetemperatureisreducedinmostofChina.
简介:我们曾报道了短梗霉菌产生的高纤维素酶产量98。在这项研究中,羧甲基纤维素酶(CMCase)在培养的细胞。短梗霉98的纯化至均一,与酶的最大产量为4.51U(mg蛋白)-1。SDS-PAGE分析表明,纯化的酶的分子量为67.0kda。具有相当的敏感性为40℃纯化的酶的最适温度,比从其他真菌的cmcases低得多。该酶的最佳pH值为5.6,和活动的个人资料被稳定在一个范围内的酸度(pH5,0-6.0)。这种酶被激活Na+,Mg2+,Ca2+,K+,Fe2+和Cu2+,然而,它是由Fe3+,Ba2+,Zn2+,Mn2+和银离子抑制。公里和纯化的酶的Vmax值4.7mgml-10.57pmolL-1min-1(mg蛋白)1,分别。只有大小不同的低聚糖,羧甲基纤维素(CMC)释放与纯化的酶水解后。该基因编码的酶是A.霉98个克隆,其中包含一个开放阅读框(eu978473)意义。推导的蛋白质含有酶超家族的保守结构域(糖基水解酶家族5)。的N-末端氨基酸序列的纯化的酶是m-a-p-h-a-e-p-q-s-q-t-t-e-q-t-s-s-g-q-f,这与从克隆的基因推导一致。这表明,纯化的酶是由克隆纤维素酶基因在酵母编码。