简介:WeusedearthquakecatalogsrecordedbyGuangdongSeismologicalNetworkfrom2008to2014toresolvethespatialvariationsofb-valuesinthecoastalareaofGuangdong,particularlyinthreekeyresearchareas(Yangjiang,Heyuan,andoffshoreNanaoIsland)withstrongseismicity.Ourresultsrevealedthatb-valuesexhibitedsignificantspatialvariations,andzoneswithlowb-valuescouldindicatethemostlikelyseismogenicareaoflargeearthquakes.Weobservedthreeclearlowb-valuepatchesintheoffshoreNanaoIsland.Wefoundadistincthighb-valuepeakatthedepthof11kmandtwominimumpeaksatabout14and7–8kmintheYangjiangarea.Theoverallb-valuesgenerallydecreasewithdepthintheHeyuanarea.Thespatialvariationsofb-valuesreflecttectonicanomalies;thatis,the‘low-high-low’distributionofb-valuesintheoffshoreNanaoIslandandtheYangjiangareamayindicatetheanomalyofthecrustalstructurewithaweaklayer.Theb-valuesofreservoir-inducedseismicityareobviouslylowerthanthatinducedbytectonism.Thisfindingindicatesthatthereservoirareaisgenerallyathighstressstateundertheconditionofhighporepressure.Weinferredthatlargeearthquakesmightbepronetooccurat10–12kmdepthintheoffshoreNanaoIsland,at12–15kmdepthintheYangjiangarea,andatthelowerpartoftheseismicactivityzoneintheHeyuanarea.Moreover,theupstreamareaoftheXinfengjiangreservoiristhemostlikelyareaoffuturelargeearthquakesintheHeyuanarea.
简介:SequencevariationofpartialcytochromebgenesbetweentwoCoiliaspecies,C.ectenesandC.mystus,wasinvestigated.Ofthe402nucleotides,twenty-seven(6.72%)arepolymorphicandallaresynonymoussubstitutions.Atthethirdpositionsofgeneticcondonofcytochromebgene,thetwospeciesshowanextremeanti-Gbias(<4%)andapronouncedbiastowardsAandC(>68%).Thereisnoaminoacidsequencedivergencebetweenthepartialcytochromebgenesofthetwospecies,indicatingaclosegeneticrelationshipbetweenthem.Thek-2pgeneticdistanceofpartialcytochromebsegmentofthetwospeciesis0.072,suggestingthatthespecieswereseparated3.6Maago,inthemiddlePliocene.Ourresultrevealsthatthecytochromebgeneisanappropriatemarkerforstudiesofpopulationgeneticstructuresandphylogeographicpatternsofthetwospecies.
简介:Effectsofthemixtureofβ-ecdysoneandpolypodine-B(2:1W/W)onthemoultingandgrowthofprawn,Penaeusorientalisswerestudiedinlaboratorybyrearingtheanimalwiththeaddedcompounddiets.Theresultsshowedthattheadditionofthemoultinghormone(M.H)mixturetocompounddiets,from1.33×10-6to30×10-6,couldincreasemoultingactivityandgrowthrateoftheprawntodifferentextents,amongwhichthe5.33×10-6-10.67×10-6additionhadstatisticallysignificanteffects.However,60×10-6addeddietscausedmoultingandgrowthinhibitionsandcuticlethickening.Theanalysesofprotein,waterandashcontentsoftheprawnshowednoremarkabledifferencesbetweenhormonedosedanimalsandthecontrol.
简介:HepatitisBvirus(HBV)isasignificantglobalpathogenandefficientcureforHBVpatientsisstillachallenginggoal.Wepreviouslyreportedthatacidicmucopolysaccharidefromstichopusjaponicusselenka(SJAMP)couldinhibitHBsAgandHBeAgexpressioninvitro.However,thepotentialanti-HBVeffectsofSJAMPinvivohavenotyetbeenexplored.Inthisstudy,weshowthatSJAMPexhibitspotentanti-HBVactivityinHBVtransgenicmiceinadose-dependentmanner.Specifically,sixtyHBVtransgenicmaleBALB/cmicewererandomlyselectedtoreceivethetreatmentofPBS,lowdoseSJAMP(30mgkg-1),middledoseSJAMP(40mgkg-1),highdoseSJAMP(50mgkg-1)andIFN(45IUkg-1)for30d.SJAMPtreatmentsuppressedserumHBV-DNA,andliverHBsAgandHBcAglevelsinHBV-transgenicmice.ThepresentstudyhighlightsthepotentialapplicationofSJAMPinHBVtherapy.
简介:Thegrowth,biochemicalcontentandbioaccumulationquantityof2,2’,4,4’-tetrabromodiphenylether(BDE-47)inSkeletonemacostatumwerestudiedunderdifferentN:Pratios(1,4,16,64and128).AllcellularbiochemicalcontentsofS.costatumpresenteddecreasingtrendovercultivationtime.Atearlystageofcultivation,thecellularprotein,carbohydrateandlipidinS.costatumpresentedhighervaluesintreatmentsofN:P=4and16.However,theywerelowerinthesetreatmentsatthelatestage,buthigherintreatmentsN:P=1and128.Similarly,BDE-47levelspercellofS.costatumwerehigherintreatmentsofN:P=4and16atearlystageofcultivation,whichwere3.8and3.7ng(106cells)-1,respectively.Atthemiddlestageofcultivation,theBDE-47levelperS.costatumcelllowered;anditfurtherreducedinthetreatmentsofN:P=4and16atthelatestagewiththevalues0.6and0.5ng(106cells)-1,respectively.However,itroseinN:P=128,reachingupto2.3ng(106cells)-1.ComparedwithBDE-47percell,BDE-47peralgalvolumeunderdifferentN:Pratiosdidnotpresentobviousdifference.ThequantityBDE-47accumulatedpercellofS.costatumwaspositivelycorrelatedwithprotein,carbohydrateandlipidpercell;meanwhile,theBDE-47pervolumehadapositivecorrelationwithbiochemicalcontentpervolume.ThevariationofbioaccumulationabilityofBDE-47inS.costatumcanbeexplainedbybiochemicalchangesduetoN:Pratios.
简介:TiO2filmswereformedonmetallictitaniumsubstratesbytheanodicoxidationmethodinH2SO4solutionunderthe80VD.C..PhasecomponentandmicrostructurewerecharacterizedbyX-raydiffraction(XRD)andscanningelectronmicroscopy(SEM).Watercontactanglesontitaniumoxidefilmsurfaceweremeasuredunderbothdarkandsunlightilluminationconditions.Corrosiontestswerecarriedoutinseawaterunderdifferentilluminationconditionsbyelectrochemistryimpedancespectrum(EIS)andpolarizationcurves.TheresultshowedthattheTiO2filmpreparedbytheanodicoxidationmethodwasanatasewithauniformstructureandwithoutobviousporesorcracksonitssurface.Theaveragewatercontactangleofthefilmwas116.4?indark,incontrasttoanangleof42.7?undertheUVilluminationfor2hours,whichdemonstratesgoodhydrophobicproperty.Theanti-corrosionbehavioroftheTiO2filmwasdecliningwiththeextendedimmersiontime.Underdarkconditions,however,thehydrophobicTiO2filmretardedthewaterinfiltratingintothesubstrate.Theimpedancechangedslowlyandthecorrosioncurrentdensitywas2ordersofmagnitudelowerthanthatwiththefilmilluminatedbysunlight.AllofthosementionedaboveindicatethattheTiO2filmpossessesmuchbetterperformanceunderdarkcondition,anditcanbeappliedasanengineeringmaterialunderdarkseawaterenvironment.
简介:我们曾报道了短梗霉菌产生的高纤维素酶产量98。在这项研究中,羧甲基纤维素酶(CMCase)在培养的细胞。短梗霉98的纯化至均一,与酶的最大产量为4.51U(mg蛋白)-1。SDS-PAGE分析表明,纯化的酶的分子量为67.0kda。具有相当的敏感性为40℃纯化的酶的最适温度,比从其他真菌的cmcases低得多。该酶的最佳pH值为5.6,和活动的个人资料被稳定在一个范围内的酸度(pH5,0-6.0)。这种酶被激活Na+,Mg2+,Ca2+,K+,Fe2+和Cu2+,然而,它是由Fe3+,Ba2+,Zn2+,Mn2+和银离子抑制。公里和纯化的酶的Vmax值4.7mgml-10.57pmolL-1min-1(mg蛋白)1,分别。只有大小不同的低聚糖,羧甲基纤维素(CMC)释放与纯化的酶水解后。该基因编码的酶是A.霉98个克隆,其中包含一个开放阅读框(eu978473)意义。推导的蛋白质含有酶超家族的保守结构域(糖基水解酶家族5)。的N-末端氨基酸序列的纯化的酶是m-a-p-h-a-e-p-q-s-q-t-t-e-q-t-s-s-g-q-f,这与从克隆的基因推导一致。这表明,纯化的酶是由克隆纤维素酶基因在酵母编码。
简介:CarboncycleisconnectedwiththemostimportantenvironmentalissueofGlobalChange.Asoneofthemajorcarbonreservoirs,oceansplayanimportantpartinthecarboncycle.Inrecentyears,ironseemstogiveusagoodnewsthatoceanicironfertilizationcouldstimulatebiologicalproduc-tivityasCO2sinkofhuman-producedCO2.Oceanicironfertilizationexperimentshaveverifiedthataddingironintohighnutrientlowchlorophyll(HNLC)seawaterscanincreasephytoplanktonproductionandexportorganiccarbon,andhenceincreasecarbonsinkofanthropogenicCO2,toreduceglobalwarming.Insixtydays,theexportorganiccarboncouldreach10000timesforaddingironbymodelpredictionandinsituexperiment,I.e.TheatmosphericCO2uptakeandinorganiccarbondrawdowninupperseawatersalsohavethesamemagnitude.Therefore,oceanicironfertilizationisoneofthestrate-giesforincreasingcarbonsinkofanthropogenicCO2.Thepaperisfocusedontheironfertilization,es-peciallyinsituoceanironexperimentsinorderthatthefutureresearchismoreefficient.
简介:Astochastictwo-dimensionalFornasini-Marchesini'sModelⅡ(2-DFMMⅡ)withmultiplicativenoiseisgiven,andafilteringalgorithmforthismodel,whichisoptimalinthesenseoflinearminimum-variance,isdeveloped.Thestochastic2-DFMMⅡwithmultiplicativenoisecanbereducedtoa1-Dmodel,andtheproposedoptimalfilteringalgorithmforthestochastic2-DFMMⅡwithmultiplicativenoiseisobtainedbyusingthestateestimationtheoryof1-Dsystems.Anexampleisgiventoillustratethevalidityofthisalgorithm.
简介:Thispaperpresentsthedetaildesignconditions,designcriteriaandmethodsaswellasdesignconclusionsoftheprocess,structureandcorrosionoftheproductionflowlinesandhighpressurewaterinjectionlinesinBZ34-2/4oilfieldinBohaiBay.AtpresentthisdesignrepresentsthedesigncapabilityandlevelinthefieldofthesubmarinepipelineengineeringofChina.
简介:Inthisstudy,thecombinedactionsofwavesandtidalcurrentsinestuarineandcoastalareasareconsideredanda2Dmathematicalmodelforsedimenttransportbywavesandtidalcurrentshasbeenestablishedinorthogonalcurvilinearcoordinates.Non-equilibriumtransportequationsofsuspendedloadandbedloadareusedinthemodel.Theconceptofbackgroundconcentrationisintroduced,andtheformulaofsedimenttransportcapacityoftidalcurrentsfortheOujiangRiverestuaryisobtained.TheDouGuorenformulaisemployedforthesedimenttransportcapacityofwaves.SedimenttransportcapacityintheformofmudandtheintensityofbacksiltingarecalculatedbyuseofLuoZaosen'sformula.Thecalculatedtidalstagesareingoodagreementwiththefielddata,andthecalculatedvelocitiesandflowdirectionsof46verticallinesfor8crosssectionsarealsoingoodagreementwiththemeasureddata.Onsuchabasis,simulationsofbacksiltingafterexcavationofthewaterwaywithasandbarundercomplicatedboundaryconditionsinthenavigationchannelinducedbysuspendedload,bedloadandmudbywavesandtidalcurrentsarediscussed.
简介:TheDiverGasRecoverySystem(DGRS)isanimportantaccessoryequipmentofthe300mSaturationDiveSystem.Thesystemfacilitatestherecyclingofthebreathinggasesfordivers,therefore,ithasgreateconomicbenefits.InApril1995,a300munmannedtestanda100mmannedtestontheHY2031DGRSwerecarriedoutsuccessively.Tenmonthslater,anothermannedtestontheHY2032DGRS
简介:AninvivofluorescencediscriminationtechniqueforphytoplanktonpopulationwasdevelopedbyusingWaveletpackettransform,clusteranalysisandnon-negativeleastsquares.Thetechniquewasusedtoanalyzewatersamplesfromdifferentsearegions.Forsimulativemixtures,whendominantspeciesaccountfor60%,70%,80%,90%atthedivisionlevel,thecorrectdiscriminationratios(CDRs)are83.0%,99.1%,99.7%and99.9%withtherelativecontentsof58.5%,68.4%,77.7%and86.3%,respectively;whenthealgaedominanceare60%,70%,80%,90%,100%atthegenuslevel,theCDRsare86.1%,94.9%,95.2%,96.8%and96.7%,respectively.Forlaboratorymixtures,theCDRsare88.1%and78.4%atthedivisionandgenuslevel,respectively.Forfieldsamples,theCDRswere91.7%and80%atthedivisionandgenuslevel,respectively(mesocosmexperiments),andtheCDRswere100%and66.7%atthedivisionandgenuslevel,respectively(JiaozhouBay).ThefluorometrictechniquewasusedtoestimatethephytoplanktoncommunitycompositionandrelativeabundanceofdifferentclassesfortheApril2010cruiseintheYellowSeawiththeresultsagreeingwiththoseinpublishedpapersbyotherauthors.
简介:Huanghe河三角洲是世界的大河之一,自从最后,Huanghe河里的Huanghe河三角洲paleoenvironmental进化是一个热问题冰川。基于联合AMS14核心DYZK1沉积标明日期在的C在亚底部侧面上Huanghe河三角洲和声学的顺序沉没了,phytolith分析在96件沉积样品上被执行。谷物尺寸参数,磁性的危险性与biostratum的垂直变化被相结合重建在Huanghe河三角洲的paleo沉积、气候的条件。在索引参数有一条重要垂直变化法律的学习结果表演,和Huanghe河三角洲的那沉积环境经历了河的facies-sealand转变的进化进程外形潮汐的完全外形浅海的faciesdelta外形自从26.0kaB。P...phytolith分析结果如下。第一,phytoliths被划分成矛尖状,光滑伸长,多刺伸长,一种特殊格律,长矩形,bulliform和其它类型。在不同sedimental环境,phytolith内容定期变化,显示phytolith在沉积环境有的一样装配稳定性的某个度。第二,矛尖状,phytoliths的光滑伸长和多刺伸长范畴在潮汐的扁平的外形和三角洲免职有更大的内容,当时一在浅海的外形的更小的内容和河的外形环境。通过变化系数的比较分析,第三,在沉积的主要phytolith范畴的内容在sealand转变外形和河的外形免职有一个更大的变化振幅,当在tidalflat外形和三角洲外形免职是更稳定的时。
简介:Inthispaper,thefocusedwavegroupswithdifferentparametersandtheiractionsonaverticalcylinderareexperimentallystudied.Theharmonicwavecharacteristicsofthefocusingwavesareanalyzedbytheadditionandsubtractionofthecrestandtroughfocusingwaves.Theanalyzedresultsshowthathigherorderharmonicscanbegeneratedbecauseoftheinteractionofcomponentwaves.Nonlinearityincreaseswiththeinputtedwaveamplitudeandthefrequencywidthincrement.Further,thewaverun-uparoundtheverticalcircularcylinderisexperimentallystudied.Itincreaseswiththewavesteepnessandtherelativecylinderdiameterincrease.However,thevariationsofwaverun-uparoundthecircularcylinderaredifferent.Theresearchesprovideareferenceforfurthernumericalstudies.
简介:TostudytheTaiwanStrait(TS),anunusualseaarea,thenumericalmodelinmarginalseasofChinaisusedtosimulateandanalyzethetidalwavemotioninthestrait.ThenumericalmodelingexperimentsreproducetheamphidromicsystemoftheM2tideinthesouthendoftheTaiwanstrait,andconsequentlyconfirmtheexistenceofthedegenerateamphidromicsystem.Onthisbasis,furtherdiscussionisconductedontheM2systemanditsformationmechanism.ItcanbeconcludedthatthetidalwavesoftheTSisconsistedoftheprogressingwavefromthenorthentranceandthedegenerateamphidromicsystemfromthesouthentrance,inwhichtheprogressingwavefromthenorthentrancedominatesthetidalwavemotioninthestrait.Exceptfortheconvergenteffectcausedbythelandformandboundary,thedegenerateamphidromicsystemproducedinthesouthofthestraitisanotherimportantfactorforthefollowingphenomena:thelargetidalrangeinthemiddleofthestrait,theconcentrativezoneofco-amplitudeandco-phaselineinthesouthofthestrait.ThedegenerateamphidromicsystemismainlyproducedbytheincidentPacificOceantidalwavefromtheLuzonstraitandtheactionbytheshorelineandlandform.Thepositionoftheamphidromicpointiscompelledtomovetowardsouthwestuntildegeneratingbythepowerfulprogressingwavefromthenorthentrance.