学科分类
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7 个结果
  • 简介:Thispaperpresentsthedetaildesignconditions,designcriteriaandmethodsaswellasdesignconclusionsoftheprocess,structureandcorrosionoftheproductionflowlinesandhighpressurewaterinjectionlinesinBZ34-2/4oilfieldinBohaiBay.AtpresentthisdesignrepresentsthedesigncapabilityandlevelinthefieldofthesubmarinepipelineengineeringofChina.

  • 标签: SUBMARINE PIPELINE SLUGGING INHIBITOR RISER inplace
  • 简介:18SribosomalDNA基因(18SrDNA)序列(近似在长度的1300bp)从从与线虫大批从Qingdao海岸的内部潮汐的沉积收集的免费生活的海洋的线虫提取的DNA被放大特定的教材。PCR产品被克隆,重新放大,与Rsa消化了我和Hin6我限制endonucleases和分开的inagarose胶化。在17种限制碎片长度类型之中,打1,2和6盖住61.2%,分别地,当时,克隆的14.4%and9.3%分析了留下14仅仅盖住21克隆,它说明了15.1%总数。24代表性的克隆andphylogenetically被定序由指在RDP和GenBank数据库当前可得到的那些分析了。尽管它是难的由于已知的海洋的免费生活的线虫的18SrDNA顺序数据的缺乏把这些序列分到已知的种类或类,获得的序列被分到Adenophorea的线虫。在他们之中,12个序列接近了PontonemavulgareandAdoncholaimussp,四到Daptonemaprocerus并且二(相同)到Enoplusbrevis。我们的结果证明免费生活的海洋的线虫差异能被检索的PCR决定,18SrDNA的分析定序,仅当免费生活的海洋的线虫的18SrDNA序列被积累到某程度,一个18SrDNA序列能被分到种或一个类。

  • 标签: 青岛海滨 线虫类 生物多样性 18S核醣体RNA 基因序列分析
  • 简介:WeusedearthquakecatalogsrecordedbyGuangdongSeismologicalNetworkfrom2008to2014toresolvethespatialvariationsofb-valuesinthecoastalareaofGuangdong,particularlyinthreekeyresearchareas(Yangjiang,Heyuan,andoffshoreNanaoIsland)withstrongseismicity.Ourresultsrevealedthatb-valuesexhibitedsignificantspatialvariations,andzoneswithlowb-valuescouldindicatethemostlikelyseismogenicareaoflargeearthquakes.Weobservedthreeclearlowb-valuepatchesintheoffshoreNanaoIsland.Wefoundadistincthighb-valuepeakatthedepthof11kmandtwominimumpeaksatabout14and7–8kmintheYangjiangarea.Theoverallb-valuesgenerallydecreasewithdepthintheHeyuanarea.Thespatialvariationsofb-valuesreflecttectonicanomalies;thatis,the‘low-high-low’distributionofb-valuesintheoffshoreNanaoIslandandtheYangjiangareamayindicatetheanomalyofthecrustalstructurewithaweaklayer.Theb-valuesofreservoir-inducedseismicityareobviouslylowerthanthatinducedbytectonism.Thisfindingindicatesthatthereservoirareaisgenerallyathighstressstateundertheconditionofhighporepressure.Weinferredthatlargeearthquakesmightbepronetooccurat10–12kmdepthintheoffshoreNanaoIsland,at12–15kmdepthintheYangjiangarea,andatthelowerpartoftheseismicactivityzoneintheHeyuanarea.Moreover,theupstreamareaoftheXinfengjiangreservoiristhemostlikelyareaoffuturelargeearthquakesintheHeyuanarea.

  • 标签: B-VALUES EARTHQUAKE GUANGDONG SEISMOGENIC area STRESS
  • 简介:SequencevariationofpartialcytochromebgenesbetweentwoCoiliaspecies,C.ectenesandC.mystus,wasinvestigated.Ofthe402nucleotides,twenty-seven(6.72%)arepolymorphicandallaresynonymoussubstitutions.Atthethirdpositionsofgeneticcondonofcytochromebgene,thetwospeciesshowanextremeanti-Gbias(<4%)andapronouncedbiastowardsAandC(>68%).Thereisnoaminoacidsequencedivergencebetweenthepartialcytochromebgenesofthetwospecies,indicatingaclosegeneticrelationshipbetweenthem.Thek-2pgeneticdistanceofpartialcytochromebsegmentofthetwospeciesis0.072,suggestingthatthespecieswereseparated3.6Maago,inthemiddlePliocene.Ourresultrevealsthatthecytochromebgeneisanappropriatemarkerforstudiesofpopulationgeneticstructuresandphylogeographicpatternsofthetwospecies.

  • 标签: 细胞色素 B基因 基因转化 线粒体DNA 凤尾鱼
  • 简介:Effectsofthemixtureofβ-ecdysoneandpolypodine-B(2:1W/W)onthemoultingandgrowthofprawn,Penaeusorientalisswerestudiedinlaboratorybyrearingtheanimalwiththeaddedcompounddiets.Theresultsshowedthattheadditionofthemoultinghormone(M.H)mixturetocompounddiets,from1.33×10-6to30×10-6,couldincreasemoultingactivityandgrowthrateoftheprawntodifferentextents,amongwhichthe5.33×10-6-10.67×10-6additionhadstatisticallysignificanteffects.However,60×10-6addeddietscausedmoultingandgrowthinhibitionsandcuticlethickening.Theanalysesofprotein,waterandashcontentsoftheprawnshowednoremarkabledifferencesbetweenhormonedosedanimalsandthecontrol.

  • 标签: PENAEUS EFFECTS of statistically larvae remarkable
  • 简介:HepatitisBvirus(HBV)isasignificantglobalpathogenandefficientcureforHBVpatientsisstillachallenginggoal.Wepreviouslyreportedthatacidicmucopolysaccharidefromstichopusjaponicusselenka(SJAMP)couldinhibitHBsAgandHBeAgexpressioninvitro.However,thepotentialanti-HBVeffectsofSJAMPinvivohavenotyetbeenexplored.Inthisstudy,weshowthatSJAMPexhibitspotentanti-HBVactivityinHBVtransgenicmiceinadose-dependentmanner.Specifically,sixtyHBVtransgenicmaleBALB/cmicewererandomlyselectedtoreceivethetreatmentofPBS,lowdoseSJAMP(30mgkg-1),middledoseSJAMP(40mgkg-1),highdoseSJAMP(50mgkg-1)andIFN(45IUkg-1)for30d.SJAMPtreatmentsuppressedserumHBV-DNA,andliverHBsAgandHBcAglevelsinHBV-transgenicmice.ThepresentstudyhighlightsthepotentialapplicationofSJAMPinHBVtherapy.

  • 标签: Stichopus japonicus acid mucopolysaccharide hepatitis B virus transgenic mice ANTIVIRAL
  • 简介:Huanghe河三角洲是世界的大河之一,自从最后,Huanghe河里的Huanghe河三角洲paleoenvironmental进化是一个热问题冰川。基于联合AMS14核心DYZK1沉积标明日期在的C在亚底部侧面上Huanghe河三角洲和声学的顺序沉没了,phytolith分析在96件沉积样品上被执行。谷物尺寸参数,磁性的危险性与biostratum的垂直变化被相结合重建在Huanghe河三角洲的paleo沉积、气候的条件。在索引参数有一条重要垂直变化法律的学习结果表演,和Huanghe河三角洲的那沉积环境经历了河的facies-sealand转变的进化进程外形潮汐的完全外形浅海的faciesdelta外形自从26.0kaB。P...phytolith分析结果如下。第一,phytoliths被划分成矛尖状,光滑伸长,多刺伸长,一种特殊格律,长矩形,bulliform和其它类型。在不同sedimental环境,phytolith内容定期变化,显示phytolith在沉积环境有的一样装配稳定性的某个度。第二,矛尖状,phytoliths的光滑伸长和多刺伸长范畴在潮汐的扁平的外形和三角洲免职有更大的内容,当时一在浅海的外形的更小的内容和河的外形环境。通过变化系数的比较分析,第三,在沉积的主要phytolith范畴的内容在sealand转变外形和河的外形免职有一个更大的变化振幅,当在tidalflat外形和三角洲外形免职是更稳定的时。

  • 标签: 黄河三角洲地区 古环境演化 植硅体 高分辨率 河流相沉积 沉积环境