简介：BACKGROUND:ToevaluateandsummarizetheeffectsofcerebralperfusionandvascularreserveonthetreatmentofSICAS.Recently,researchonβ-amyloidproteinhasfocusedontheregulatoryeffectsofes-trogenorphytoestrogenonitsdeposition.However,therehavebeenonlyafewreportsondynamicchangesofβ-amyloidproteindepositioninhippocampusofovariectomizedrats.OBJECTIVE:Tomeasureβ-amyloidproteindepositioninthehippocampalformationofovariectomizedratsbyusingimmunohistochemistry;toobservetime-dependentdynamicchanges.DESIGN:Randomizedcontrolledanimalstudy.SETTING:ThirdXiangyaHospitalofCentralSouthUniversity.MATERIALS:TheexperimentwascarriedoutintheCentralLaboratoryoftheThirdXiangyaHospitalofCentralSouthUniversityfromNovember2005toDecember2006.FiftyhealthyfemaleSpragueDawley(SD)rats,weighing(293±10)g,wereprovidedbytheAnimalLaboratoryofXiangyaMedicalCollege,CentralSouthUniversity.Allratshadneitherachildbearinghistorynorhepaticorrenaldisease,orskeletaldeformity.β-amyloidproteinimmunohistochemicalkitwasprovidedbyWuhanBosterCompany.Theex-perimentwasinaccordancewithanimalethicsstandards.METHODS:Allratswererandomlydividedintofivegroups,includingnormalcontrolgroup(n=10),shamoperationgroup(n=10),andovariectomizedgroup(n=30).Afteranesthesiaintheovariectomizedgroup,thebilateralovarieswereseparatedandresected.Thesamevolumeoffatwasresectedintheshamoperationgroup.Ratsfromthenormalcontrolgroup,however,didnotreceiveanysurgicaltreatments.Ratsinthenormalcontrolgroupandshamoperationgroupweresacrificedbyanesthesia7weeksaftersurgery.Everytenratsfromtheovariectomizedgroupwasrespectivelysacrificedat7,15,and30weeksaftersurgery.Immunohistochemistrywasusedtodetectβ-amyloidproteindepositioninhippocampalsections.Cellcountingandgrayvaluemeasurementsservedtorecordthedynamicchangesin�