简介：AIM:Tostudytheblockingeffectsofgenisteinoncellproliferationcycleinhumangastriccarcinomacells(SGC-7901)andthepossiblemechanism.METHODS:MTTassaywasappliedinthedetectionoftheinhibitoryeffectsofgenisteinoncellproliferation.Flowcytometrywasusedtoanalyzethecellcycledistribution.ImmunocytochemicaltechniqueandWesternblottingwereperformedtodetecttheproteinexpressionofcyclinD1,cyclinB1andp21waf1/cip1.RESULTS:Genisteinsignificantlyinhibitedthegrowthandproliferationofhumangastriccarcinomacells(SGC-7901).Sevendaysaftertreatmentwithdifferentconcentrationsofgenistein(2.5,5.0,10.0,20.0μg/mL),thegrowthinhibitoryrateswere11.2%,28.8%,55.3%,84.7%respectivelyandcellcycleswerearrestedattheG(2)/Mphase.GenisteindecreasedcyclinD1proteinexpressionandenhancedcyclinB1andp21waf/cip1proteinexpressioninaconcentration-dependentmanner.CONCLUSION:ThegrowthandproliferationofSGC-7901cellscanbeinhibitedbygenisteinviablockingthecellcycle,withreducedexpressionofcyclinD1andenhancedexpressionofcyclinB1andp21waf/cip1proteinintheconcentrationrangeof0-20μg/mL.

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简介：TheanalysisofsolarcellperformancehasbeendonebysimulatingtheexternalI-Vcharacteristicsofn^+/p/p^+singlecrystalsiliconsolarcellunderhighlightintensityand1.5airmass(AM).Thismethodallowsthemaximizationofsolarcellefficiency.Tofabricatelow-costn^+/p/p^+singlecrystalsiliconsolarcells,solidsourceofdopedphosphorousandboronwasused.

简介：AmethodisdescribedwhichpermitstransmissionelectronmicroscopeofsinglecellstreatedwithHpDpluslasermicroirradiation.Thepreselectedsinglecellthatwasirradiatedbylaserunderlightmicroscopeandfollowedfixation,embeddedandsectioningisexaminedunderelectronmicroscope.Theresultsdemonstratedthatatthelightdoseof1.88ml/μm2notonlytheirradiatednucleolusappearedtransparentregion,buttheotherpartssuchasnon-irradiatedmitochondriaincytoplasmcanalsobedamaged.Whenpartialcytoplasmisirradiatedwiththelightdoseof4.50ml/μm2,thedamagesappearinallcytoplasm,butthereislittlechangeinthenucleus.Theexperimentalresultsalsodemonstratethatcytoplasmismoresensitivethannucleus.ItisthemitochondriaincytoplasmthatareverysensitivetoHpDpluslaser.

简介：AbstractBackground:Deregulation of miRNA-21 expression has been reported to be associated with vascular smooth muscle behavior and cytoskeletal stability. This study is aimed to investigate the density of serum miRNA-21 in patients with different phases of intracranial aneurysms (IAs) and explore its warning function for IA rupture.Methods:A total of 16 in 200 IA patients were selected and categorized into 4 groups based on the phase of IA. Microarray study was carried out using serum miRNA and differentially expressed miRNAs were identified. Another 24 samples from a cohort of 360 patients were added and real-time polymerase chain reaction (RT-PCR) was performed on expanded sample size (n = 40) for miRNA-21 validation. Potential gene targets of miRNA-21 were screened out from Gene Ontology (GO) database and literatures.Results:Microarray study identified 77 miRNAs with significantly different expression levels between experimental groups and the control group. RT-PCR assays validated significant downregulation of miRNA-21 in experimental groups, among which miRNA-21 expression level of daughter aneurysm group decreased the most. Bioinformatic analyses revealed that several target genes related with miRNA-21 may be involved in IA formation and rupture.Conclusions:This study suggested that miRNA-21 had a protective effect for intracranial vascular wall against remodeling and warning function for intracranial aneurysm rupture. Significant suppression of serum miRNA-21 in IA patients may provide diagnostic clues for aneurysm rupture and guide clinical intervention.

简介：Tocloneandconstructtherecombinantplasmidcontainingthemajoroutermembraneprotein(MOMP)geneofChlamydiatrachomatis(C.trachomatis)andtoexpressthefusionproteininE.coliBL21,theMOMPgenewasamphfiedbypolymerasechainreaction(PCR)fromgenomeofC.trachomatisserovarD.ThefragmentwasclonedintotheprokaryoticexpressionvectorpET-22b(+)afterdigestionwithBamHⅠandNotⅠandtransformedintoE.coliXL1-Blue.RecombinantswereselectedbyenzymedigestionandsequencingandtherecombinantplasmidwithMOMPgenewasthentransformedintoE.coliBL21withIPTGtoexpressthetargetgene.TheexpressionrecombinantproteinswerepurifiedbyNi-NTAaffinitychromatography,andidentifiedbySDS-PAGEandWesternblot.Itwasfoundthata1.2kbMOMPgenewasisolated.TheDNAsequenceofMOMPwasfoundtobejustthesameasthesequencepublishedbyGenBank.ArecombinantplasmidcontainingMOMPgenewasconstructedtoexpressthefusionproteinsinE.coli.SDS-PAGEanalysisshowedthattherelativemolecularweightoftherecombinantproteinwasabout47kDathatwasconsistentwiththetheoreticalpredictedvalue,andthespecificityoftheexpressedproteinwasconformedbyWesternblot.ItconcludedthattheMOMPgenecouldbeexpressedintheprokaryoticsystem,bywhichitprovidedthefoundationforthefuturestudiesonthebiologicalactivitiesofC.trachomatisandforthedevelopmentofvaccineagainstthispathogen.

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简介：性腺开发要求保证germline和体的干细胞的更新和区别最终生产成熟配偶子的一个协调soma-germline相互作用。果蝇瘤suppressor基因磁盘大(dlg)编码在神经与肌的连接的上皮的极化，不对称的神经母细胞部门，和形成期间工作的有中隔的连接蛋白质。这里，我们在体的包囊房间(SCC)在睾丸开发和它的批评功能报导dlg的角色。在这些房间，dlg首先为他们的幸存和扩大被要求，并且贡献spermatocyte包囊区别。当Dlg变得限制了在时，房间死亡首先在spermatogonial扩大的结束发生在SCC野类型(wt)睾丸到盖住成长spermatocyte包囊的远侧的体的房间。在早SCC的dlg抄本的RNAi弄空充分阻止了睾丸开发，而在迟了的SCC的弄空导致了spermatocyte包囊结构和细菌房间individualization的一个故障。在SCC的特定的dlg表示导致了dlg异种睾丸的发展营救，而它在细菌房间的表示没施加如此的效果。在wt睾丸的dlgoverexpression在损坏spermatogonial包囊的情况下导致了spermatocyte包囊扩大。我们的数据证明dlg实质上为他们的幸存，扩大，和区别，并且为germline房间的封装在SCC被要求。

简介：ObjectiveTounderstandthemechanismofnoiseexposureinducedouterhaircells(OHCs)deathpathways.MethodsThirtytwoguineapigswereusedinthisstudy.Theanimalswereeitherexposedfor4h/daytobroadbandnoiseat122dBSPL(A-weighted)for2consecutivedaysorperfusedwithMNNG.Afterauditorytest,thecochleaeofanimalsweredissected.Propidiumiodide(PI),aDNAintercalatingfluorescentprobe,wasusedtotracemorphologicalchangesinOHCnuclei.F-actinstainingwasusedtodeterminemissingOHCs.Caspase-3wasdetectedinlivingorganofCortiwholemountsusingthefluorescentprobe.ThesinglestrandDNA(ssDNA)inapoptoticOHCsinguineapigsandapoptosisinducingfactor(AIF)inhaircellsinguineapigswereexaminedbyimmunohistologymethod.WholemountsoforganofCortiwereprepared.Morphologicalandfluorescentchangeswereexaminedunderaconfocalmicroscope.Results(1)Bothapoptoticandnecrotichaircellsappearedfollowingnoiseexposure.(2)NoiseexposureinducedsinglestrandDNAinapoptoticOHCsbutnotinthenormalOHCs.(3)EitherafternoiseexposureorafterMNNGperfusion,apoptoticOHCswerefeaturedbynuclearcondensationorfragmentationwithcaspase-3activation,whereasnecroticOHCswerecharacterizedbynuclearswellingwithoutcaspase-3activation.(4)InnormalorganofCorti,AIFwaslocatedinthemitochondriaareas.Afternoiseexposure,AIFwastranslocatedfrommitochondriainapoptoticandnecroticOHCs.ConclusionThesefindingsindicatethatnoiseexposuredamagesDNAintheOHC,whichtriggersactionofCaspase-3.Subsequently,AIFistranslocatedtothenucleus,leadingtoDNAdamageandOHCsdeath.

简介：AbstractWith the development of human assisted reproductive technology (ART), an objective, accurate, and non-invasive method to assess the quality and viability of oocytes and embryos remains one of the most significant goals. Granulosa cells (GCs) play an essential role in oocyte development. GCs can differentiate into mural GCs (MGCs) and cumulus cells (CCs) under the influence of oocytes. MGCs promote the growth and development of follicles by secreting cytokines and steroid hormones. Simultaneously, CCs can form cumulus-oocyte complexes to communicate with oocytes through gap junctions and promote oocyte growth and maturation. Seeking suitable biomarkers in GCs provides a direction for the non-invasive assessment of oocyte and embryo abilities during ART procedures. To date, only a few studies have investigated potentially effective GC biomarkers during ART processes, such as the apoptosis of GCs, transcriptomic characteristics of GCs, quality and quantity of mitochondria in GCs, and telomere length of such cells. These are potential reference indices for screening high-quality oocytes and embryos. Independent studies on MGCs and CCs can provide more effective results. Although there is scope for optimization and improvement, the results have become increasingly accurate with the constant advances in technology. Due to the heterogeneity of the study population and technical limitations, clinical tests for GCs cannot be performed as part of routine tests, but their prospects are promising. This article reviews the biomarkers that have been studied in MGCs and CCs.

简介：Cellaffinityisanimportantfactortobeconcernedwhenbiodegradablepolymericmaterialsareutilizedascellscaffoldintissueengineering.Manystudieshaveprovedthathydrophilicity,surfaceenergy,chargeandroughnessofthematerialsurfacegreatlyinfluencethecellattachmentandcellgrowthonthematerial.

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简介：One-cellmouseembryosfromKMstrainandB6C3F1strainwereculturedinM16medium,inwhich2-cellblockgenerallyoccurs.EmbryosofKMstrainexhibited2-cellblock,whereasB6C3F1embryos,whichareregardedasanonblockingstrain,proceededtothe4-cellstageinourculturecondition.Itisoftenassumedthattheblockofearlydevelopmentisduetothefailureofzygoticgeneactivation(ZGA)inculturedembryos.Inthisstudyweexaminedproteinsynthesispatternsbytwo-dimensionalgelelectrophoresisof[35S]methionineradiolabeled2-cellembryos.Embryosfromtheblockingstrainandthenonblockingstrainwerecomparedintheirdevelopmentbothinvitroandinvivo.ThedetectionofTRCexpression,amarkerofZGA,at42hposthCGinKMembryosdevelopedinvitrosuggestedthatZGAwasalsoinitiatedeveninthe2-cellarrestedembryos.Nevertheless,asignificantdelayofZGAwasobservedinKMstrainascomparedwithnormallydevelopedB6C3F1embryos.AttheverybeginningofmajorZGAasearlyas36hposthCG,TRChasalreadybeenexpressedinB6C3F1embryosdevelopedinvitroandKMembryosdevelopedinvivo.Butfor2-cellblockedKMembryos,TRCwasstillnotdetectableevenat38hposthCG.Theseevidencessuggestthat2-cell-blockedembryosdoinitiateZGA,andthat2-cellblockphenomenonisduenottothedisabilityininitiatingZGA,buttoadelayofZGA.

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简介：TheaimofthisstudyistofindtheexperimentalevidencethattheprecursorfrequencyofalloreactiveCTLsisproportionaltothenumberoftheT-cellepitopespecificities.ThenumberofT-cellepitopespecificitieswasmanipulatedbypulsingdifferentnumberofHLA-A2restrictedpeptide(s)ontotheT2cells,whichactedasstimulatingcellstoelicitallo-reactionbyco-culturingwithperipheralbloodlymphocytes(PBLs)ofHLA-A2negativeindividual.TenHLA-A2restrictedpeptides(allwerenormalcellcomponents)weresynthesized,andcellpeptideextractwaspreparedbyfrozenandthawed.T2cellsloadedwithdifferentnumberofpeptide(s)wereco-culturedwithPBLsofanHLA-A2negativeindividual;thelatterwerestainedwithPKH67inadvance.Thentheproliferationwasmonitoredwithflowcytometry,andtheprecursorfrequencyoftheeffectorcellswasanalyzedbytheModFitSoftware.After6dofculture,noproliferationwasobservedinthebulkcultureofPBLalone,andobviousproliferationtookplacewhenPBLsoftheHLA-A2negativewereco-culturedwithT2cellsloadedwithorwithoutloadingpeptide(s).TheprecursorfrequencyofthealloreactiveCTLswas0.052819forco-culturewithT2cellsloadedwithoutpeptide;howeveritwas0.030429forT2cellswithEBV/LMP2Aand0.030528forT2cellsloadedwithasingleautogeneicpeptide,andincreasedupto0.144942forT2cellsloadedwith10autogeneicpeptides;theprecursorfrequencywas0.203649whenco-culturedwithT2cellsloadedwithmiscellaneouspeptidesextractedfromthecytoplasmofT2cells.ThisstudyrevealsthattheprecursorfrequencyofalloreactiveCTLsisproportionaltothenumberofT-cellepitopespecificities,andindependentofthedensityoftheallogeneicHLAClassⅠmolecule.OurfindingssupportthehypothesisthatthealloreactiveTcellpopulationscomprisemiscellaneousTcellclones;eachisspecifictocorrespondingpMHC.ThenovelconstellationofpeptidespresentedbyallogeneicMHCmoleculesmakesthous

简介：AIM:Toevaluatetheroleofsurvivinandcaspase-3inapoptosisofgastriccarcinoma,aswellasinprognosisofpatientswithgastriccarcinoma.METHODS:Expressionsofsurvivinandcaspase-3wereinvestigatedimmunohistochemicallyin80gastriccarcinomapatientswithoutahistoryofchemo-radiationtherapy.TumorcellapoptosiswasexaminedbyTUNELmethod.RESULTS:Immunohistochemicalanalysisshowedthatsurvivinexpressionwaspositivein61of80patients(76%)withgastriccarcinoma.Incontrast,noexpressionofsurvivininadjacentnormaltissueswasdetected.Expressionlevelofcaspase-3washigherinnormaltissuesthanincarcinoma.Patientswithhigherexpressionofsurvivinhadworsehistologicalgradesandpathologicalstages.Expressionofcaspase-3wassignificantlyassociatedwithhistologicalstages,butnotwiththepathologicalstages.Althoughsurvivinexpressionincarcinomawasnotinverselyrelatedtocaspase-3,patientswithsurvivin(-)andcaspase-3(+)hadthemaximumapoptosisindex.CONCLUSION:Expressionlevelofsurvivinwasassociatedwithhistologicalgradesandpathologicalstagesofthetumor,indicatingthatsurvivinmaybeapoorprognosisfactorforgastriccarcinoma.Unlikecaspase-3,survivin(anapoptosisinhibitor)canmarkedlyinhibittheapoptosisoftumorcells.

简介：GliomacelllineC6culturedonsiliconsurfacesmodifiedbydifferentchemicalfunctionalgroups,includingmercapto（-SH）,carboxyl（-COOH）,amino（-NH2）,hydroxyl（-OH）andmethyl（-CH3）groups,wasstudiedheretoinvestigatetheinfluenceofsurfacechemistryonthecellproliferation,adhesionandapoptosis.AFMconfirmedthesimilarcharacteristicofdifferentfunctionalgroupsoccupation.TheadheringC6exhibitedmorphologicalchangesinresponsetodifferentchemicalfunctionalgroups.TheC6adheredto-COOH,-NH2,-OHand-CH3surfacesandflattenedmorphology,whilethoseon-SHsurfaceexhibitedthesmallestcontactareawithmostlyroundedmorphology,whichledtothedeathofcancercells.TheresultsofMTTassayshowedthatthe-COOHand-NH2groupspromotedceilproliferation,whilethe-SHsignificantlyinhibitedtheproliferation.Comparedwithotherchemicalfunctionalgroups,the-SHgroupexhibiteditsuniqueeffectonthefateofcancercells,whichmightprovidemeansforthedesignofbiomaterialstopreventandtreatglioma.

简介：优化协调控制的过程被划分成二个阶段。在第一个阶段，学习改进单个点的交叉的一个柔韧的最佳的控制模型优化周期和裂口。在第二个阶段，学习作为一个完全的系统把所有连接与动脉的道路的交叉相结合，并且使用房间传播模型在城市的庆祝动脉的道路上模仿交通流动。我们基于站台建议一个协调控制模型优化在邻近的交叉之间的偏移量。基因算法被MATLAB执行解决模型。性能评估表演模型有效地，还原剂一般水准延期和动脉的道路上并且大部分的车辆的停止的率不仅提高动脉的道路的交通能力，而且为流动变化降低信号控制的敏感。

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简介：Agiantcelltumoroccursmainlyintheproximaltibia,humerus,distalradiusboneandthepelvicbone.Itisrarelyobservedinsuchsitesastheribsandthetemporalbone.Theconditionisprimarilytreatedwithsurgicalexcisionandfunctionalreconstruction.Theeffectofchemotherapyonlungmetastasesandlocallyadvancedgiantcelltumorshasremainedunknown.Wecollectedandanalyzedthedataofsixpatientswithraregiantcelltumorslocatedintheheadandneckpatients.Afteranaveragefollow-upof42.6monthsaftersurgery(14to90months),nolocalrecurrenceormetastasiswasobserved.Wealsocollectedandanalyzedthedataoffivepatientswithmetastaticgiantcelltumorswhowereundergoingsurgeryfortheprimarytumorbefore;ofthreepatientswhohadexperiencedmultiplechemotherapycycles,onehadspontaneousregression,andonesurvivedforlongtimerdespiteprogression.Theothertwopatientshadtheirmajormetastaticlesionsresectedbysurgery,andpresentedlong-termsurvivalduringthefollowup.Inaddition,thisstudyreportsonepatientwithlocallyadvancedgiantcelltumoroftherib,whohasundergonesuccessfulsurgicalresectionfollowingtwocyclesofchemotherapywithifosfamideandliposomaldoxorubicin.Completeresectionofthelesionattheheadandneckisthekeytorelapse-freesurvival.Theprognosisoflungmetastasesinpatientswithgiantcelltumorsisrelativelysatisfying.Neoadjuvantchemotherapyisalsoconducivetothesurgeryforlocallyadvancedlesionsandimprovementofthequalityoflife.