简介:【摘要】目的 研究寻找 CT-SIM增强定位扫描的最佳时相。方法 本文以盆腔病变部位为例,选取我科 2017年 12月 -2018年 3月期间收治的 48例盆腔病变部位的患者,分为两组: A组采用髂外静脉 CT值监测激发扫描 26例, B组采用髂外动脉经验值延迟激发扫描 22例,测量对比两组患者 CT-SIM定位增强图像髂外动、静脉的平均 CT值。结果 A组采用髂外静脉 CT值监测激发扫描的患者的增强断层图像髂外动脉、静脉的平均 CT值 157±8.7HU高于 B组的平均 CT值 128士 8.4HU( P< 0.05)。结论 采用靶静脉 CT值监测对放疗靶区的勾画及动、静脉双期显影和病变部位强化有更加准确,更加个体化、量化的优势。
简介:SignificantanomalieswereobservedatthegeomagneticstationsinthesouthwestregionofChinabeforetheYingjiangMS6.1earthquakeandtheLudianMS6.5earthquakein2014.Weprocessedthegeomagneticverticalcomponentdiurnalvariationdatabythespatialcorrelationmethod.TheresultsshowthatduringtheperiodfromApril1toMay20,2014,thereexistedquasi-synchronousdecreasechangesinthecoefficientcurvesbetweenthefivegeomagneticstationsofGuiyang,Hechi,Nanshan,Muli,YongningandXinyiandHongshanstations.Furthermore,therewasahighgradientzoneinthenormalizedcorrelationcoefficientcontourmapwithbackgroundvaluesremoved.TheepicentersoftheYingjiangMS6.1earthquakeandtheLudianMS6.5earthquakearelocatedinthegradientzoneornearthegradientzone.
简介:Theearthquakestress-dropvaluesoftwosequenceswereaccuratelycalculatedaftertakingawaytheeffectsduetoregionalearthquakeanelasticattenuationandstationsiteresponse,usingwaveformdataandseismicphasedataofsequencesoftheJingguM_S6.6,andLudianM_S6.5earthquakesinYunnan.Theseresultsshowthatthestressdropwithmagnitudeincreaseswithinthescopeofthisstudyofmagnitude.Aftereliminatingtheinfluenceofthemagnitude,theaveragevalueofstress-dropintheJinggusequenceishigherthanthatoftheLudiansequenceatthesamemagnituderange.Thismayberelatedtothestressstateindifferentregions.Intermsofthechangesoftimeandspaceofstress-drop,beforeM_S5.8strongaftershock,thestress-dropis'slowingdown-turningup-keepingahighvalue'afterthemainshock,meanwhile,almostalloftheabnormallyhighstressdropvalueisdistributedaroundtheM_S5.8strongaftershock,showingthatthestressenvironmentintheregionwasincreasingafterthemainshock.AndaftertheM_S5.9strongaftershock,stress-droprapidlydeclinestoarelativelystablestate,meanwhile,thehighvalueofstress-dropisdistributedaroundthestrongaftershock,showingthattheregionaltectonicstressgetsmorefullyrelease,itsstressenvironmentbeginstorapidlydecrease.FortheLudiansequencewithoutastrongaftershockoccurring,theaveragevalueofstressdropislowerthanthatoftheJingguearthquakesequenceatthesamemagnituderange,whileatthesametime,thestress-dropoftheaftershocksequencealmosthasn'tchangedmuch.Inthetimeafterthemainshock,combinedwiththereleasecharacteristicsofthemainenergy,thestressintheregionisexcessivelyreleased,thesubsequentstressintheregiongraduallyreturnstonormal.ThismaybethereasonwhytheactivityofLudianaftershockssignificantlywasweakerandsubsequentlytherewerenostrongaftershocksoccurred.
简介:目的:建立HPLC-MS/MS法测定人尿液中依普利酮的浓度。方法:用蛋白质沉淀法处理尿液,采用WatersSymmetryC18色谱柱(50mm×4.6mm,5μm),流动相为甲醇(A)-乙腈(B)-1mmol/L乙酸铵水溶液(C),梯度洗脱(0.01min:15%A,85%C;0.8min:100%A;2.1min50%A,50%B;2.11~3.5min:15%A,85%C);流速:1.0ml/min,柱温:40℃,采用电喷雾离子源,正离子扫描模式检测。考察该方法的专属性、标准曲线、定量下限、精密度、回收率、基质效应和稳定性。结果:依普利酮尿液浓度在5~2000ng/ml范围内线性关系良好(r〉0.9968),定量下限为5ng/ml。批内精密度RSD〈4.31%,批间精密度RSD〈6.69%,提取回收率在94.41%~98.47%,基质效应在83.97%~99.22%(n=6)。结论:该法准确、灵敏,重复性好,可用于依普利酮在人尿液中浓度的测定。
简介:目的建立超高效液相色谱-串联质谱(UPLC—MS/MS)法测定人血中雪上一枝蒿甲素含量的方法。方法样品经乙酸乙酯提取后,Ci8柱分离,以0.1%甲酸乙腈-0.1%甲酸水为流动相梯度洗脱,正离子-多反应离子监测模式(ESI+-MRM)测定雪上一枝蒿甲素,定性定量离子对分别为344.3/58.0、344.3/91.0。结果雪上一枝蒿甲素在3.5~850ug/L-1。范围内与峰面积呈现良好的线性关系(r=-0.9968),检测限为0.1Iμg/L-1,日内、日间精密度均〈10%,低、中、高三个浓度下准确度(n=5)为97.2%-115.2%,回收率(n=5)为86.6%~89.4%。结论该方法操作简便,结果准确,可作为测定人血中雪上一枝蒿甲素含量的方法。
简介:摘要:目的:建立一个用液相色谱-质谱联用法测定中药材中黄曲霉毒素含量的方法。方法:色谱柱:thermo Scientific Hypersll GOLDTM(1.9μm,2.1×100mm);流动相: 乙腈-10mmol/L的甲酸铵水溶液,梯度洗脱;流量: 0.3 ml/min;以三重四极杆串联质谱仪检测;柱温:25 ℃。结果:根据药材基质的不同,优化得出相应满足方法要求的前处理方法并进行了验证。结论:该方法可用于中药材中黄曲霉毒素含量的测定。
简介:目的运用高效液相色谱-高分辨飞行时间质谱联用(HPLC-TOF-MS)技术和高效液相色谱串联三重四级杆质谱(HPLC-MS/MS)法,对产复康颗粒化学成分进行快速鉴定及定量分析,并进行初步药动学研究。方法HPLC-TOF-MS法采用ZORBAXEclipsePlusC18(100mm×3.0mm,1.8μm)色谱柱;流动相为乙腈(A)-0.1%甲酸水(B),梯度洗脱,体积流量0.2mL/min;质谱采用正、负离子扫描,扫描范围m/z100~2000。HPLC-MS/MS法采用WatersXBridge?BEHC18(150mm×4.6mm,2.5μm)色谱柱;流动相为乙腈(A)-0.1%甲酸水(B),梯度洗脱,体积流量0.4mL/min;ScheduledMRM正负离子切换监测模式定量分析。ig给予SD大鼠产复康颗粒(0.5、5g/kg),给药后于不同时间点颈静脉取血0.2mL,分离血浆,HPLC-MS/MS法检测产复康颗粒入血成分及含量。结果HPLC-TOF-MS法鉴定产复康颗粒中24种成分;建立的HPLC-MS/MS方法分离度良好,各方法学验证均符合要求,对13种主要成分进行定量分析,水苏碱、黄芪甲苷和益母草碱的含量较高,可能是其主要活性成分;药动学结果显示,仅在给药剂量5g/kg时,血浆中可检测到水苏碱和益母草碱,水苏碱的消除较慢,益母草碱快速消除。结论该方法简便、准确,重复性好,可用于产复康颗粒中多种成分的鉴定和含量的测定,为药材的质量控制、药动学研究提供了参考。
简介:AbstractBackground:The human brain is the most complex organ in the body, and it is important to have a better understanding of how the protein composition in the brain regions contributes to the pathogenesis of associated neurological disorders.Methods:In this study, a comparative analysis of the frontal and temporal cortex proteomes was conducted by isobaric tags of relative and absolute quantification (iTRAQ) labeling and two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS). Brain protein was taken from relatively normal tissue that could not be avoided of damage during emergent surgery of the TBI (traumatic brain injury) patients admitted in Beijing Tiantan Hospital from 2014 to 2017. Eight cases were included. Four frontal lobes and 4 temporal lobes proteome were analyzed and the proteins were quantitated. Gene Ontology (GO), Ingenuity Pathway Analysis (IPA), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to analyze the biological function of identified proteins, unchanged proteins, and differentially expressed proteins (DEPs).Results:A total number of 2127 protein groups were identified in the frontal and temporal lobe proteomes. A total of 1709 proteins could be quantitated in both the frontal and temporal cortex. Among 90 DEPs, 14 proteins were screened highly expressed in the temporal cortex, including MAPT, SNCG, ATP5IF1, GAP43, HSPE1, STMN1, NDUFS6, LDHB, SNCB, NDUFA7, MRPS36, EPDR1, CISD1, and RALA. In addition, compared to proteins expressed in the frontal cortex, 14 proteins including EDC4, NIT2, VWF, ASTN1, TGM2, SSB, CLU, HBA1, STOM, CRP, LRG1, SAA2, S100A4, and VTN were a low expression in the temporal cortex. The biological process enrichment showed that unchanged proteins between the frontal and temporal cortex mainly take part in regulated exocytosis, axon guidance, and vesicle-mediated transport. The KEGG pathway analysis showed that unchanged proteins between the frontal and temporal cortex mainly take part in oxidative phosphorylation, carbon metabolism, Huntington's disease, and Parkinson's disease.Conclusions:The majority of proteins are unchanged between the frontal and temporal cortex, and unchanged proteins are closely related to its function. Among DEPs, MATP (tau) is upregulated in the temporal cortex, closely related to Alzheimer's disease (AD), and is one of the targets for the treatment of AD. CLU is downregulated in the temporal cortex which functions as an extracellular chaperone that prevents aggregation of non-native proteins. It was suggested that the temporal lobe may not be the "functional dumb area" of the traditional view, but could be involved in important neural metabolic circuits.
简介:目的:采用HPLC-MS/MS法分析鉴定罗通定在大鼠胆汁中的主要代谢产物。方法:罗通定灌胃给予大鼠(120mg·kg^-1),收集胆汁样品。色谱分离采用ZorbaxSBC18(150mm×0.5mmI.D.,5μm);流动相:甲醇-水-三乙胺(30:70:0.07,V/V/V);质谱分析采用(+)ESI模式。结果:在大鼠胆汁中观察到4个罗通定代谢产物,初步推测其中2个为罗通定去甲基化后再与硫酸结合的产物。结论:去甲基化后形成硫酸结合物为罗通定在大鼠胆汁中一主要排泄形式。