简介:Thepresentstudywasdesignedtoperformstructuralmodificationsofofneobavaisoflavone(NBIF),usinganinvitroenzymaticglycosylationreaction,inordertoimproveitswater-solubility.TwonovelglucosidesofNBIFwereobtainedfromanenzymaticglycosylationbyUDP-glycosyltransferase.TheglycosylatedproductswereelucidatedbyLC-MS,HR-ESI-MS,andNMRanalysis.TheHPLCpeakswereintegratedandtheconcentrationsinsamplesolutionswerecalculated.TheMTTassaywasusedtodetectthecytotoxicactivityofcompoundsincancercelllines.Basedonthespectroscopicanalyses,thetwonovelglucosideswereidentifiedasneobavaisoflavone-4′-O-β-D-glucopyranoside(1)andneobavaisoflavone-4′,7-di-O-β-D-glucopyranoside(2).Additionally,thewater-solubilitiesofcompounds1and2wereapproximately175.1-and4031.9-foldhigherthanthatofthesubstrate,respectively.Amongthetestcompounds,onlyNBIFexhibitedweakcytotoxicityagainstfourhumancancercelllines,withIC50valuesrangingfrom63.47to72.81μmol·L~(-1).Theseresultssuggestthatinvitroenzymaticglycosylationisapowerfulapproachtostructuralmodification,improvingwater-solubility.
简介:摘要:为提高数字X射线平板探测器图像数据吞吐量,同时保证数据稳定可靠,提出优化改进型的可靠UDP协议。连接管理方式为探测器进行UDP广播通知计算机建立连接并周期性进行消息发送以进行保活。针对图像数据传输提出确认机制、重传机制和流量控制方案,稳定可靠地传输图像数据。命令传输针对各种不同丢包逻辑设计重传方案确保可靠。整体方案数据传输稳定可靠,图像数据流吞吐量稳定在700Mbps以上。
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