简介：客观：到为在非细胞组成的牛的心包的准备的最好的过程的搜索，为构造织物工程方法提供脚手架给那么:牛的心包与5个方法被对待，它被划分成6groups.GroupA:新鲜bovinepericardium；GroupB:Trypsin-detergentgroup；GroupC:Freeze-thaw-detergent24h组；组D:.Freeze-thaw-detergent48h组；组E:Freeze-thaw-nuclease组；组F:净化核酸酶的group.Then，由他观察th的染色并且扫描的电子显微镜

简介：基本上在在vitro和细胞外的矩阵种相关房间上面做的织物工程。织物工程的一个主要目标到preparate多孔为房间的三尺寸脚手架增殖，移居，differention并且形成理想的织物和机关的结构。在这研究，各种各样的内容的效果和到chitosan的木钉的macromolecular重量被调查并且评估。chitosan的毛孔形态学被改变集中控制，PEG.Chitosan多孔的支架的macromolecular重量有interconecting孔。毛孔形态学能与改变木钉集中和macromolecular重量被控制。毛孔尺寸在之间1050嗯，在水里胀大的度是85.70%。

简介：Objective:Tostudycellularcompatibilityofimprovedscaffoldmaterialwithdeproteinizedheterogeneousboneandprovideexperimentalbasisonchoosingthescaffoldmaterialinbonetissueengineering.Methods:Bonemarrowstromalcells(BMSC)wereco-culturedwithheterogeneousdeproteinizedboneinvitro.Thecontrastphasemicroscope,scanningelectronmicroscope,MTTassay,flowcytometrywereperformedandtheBGPcontentandALPactivitiesweredetectedinordertoobservethecellgrowth,adhesioninthematerial,cellcycleandcellviability.Results:Thescaffoldmaterialofdeproteinizedheterogeneousbonehadnoinhibitoryeffectoncellularproliferation,differentiationandsecretionfunctionofBMSCs.Conclusions:TheestablishedheterogeneousdeproteinizedbonehasgoodbiocompatibilitywithBMSCsandisapotentiallyidealscaffoldmaterialforbonetissueengineering.

简介：FUNCTIONSOFCELLSSCAFFOLDINTHETISSUEENGINEERINGCell,cellsscaffoldandtheconstructionoftissueandorganarethreemainfactorsfortheTissueEngineering.Amainfunctionofcellsscaffoldintissueengineeringistoprovideanenvironmentforcellspropagation.

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简介：ThispaperaimstoprepareaPVA-GAG-COLcompositewithpolyvinylalcohol(PVA),glycosaminoglycan(GAG)andcollagen(COL)bythemethodoffreezedryingandtoinvestigatethefeasibilityasatissueengineeringscaffoldfortissueororganrepairing.Inthisstudy,SEMwasusedtoobservethemorphology.Biocompatibilitywastestedbycellculturewiththeextractedfluidofcompositematerials.DifferentproportionalscaffoldscouldbeobtainedwithdifferentconcentrationsandalcoholysisdegreeofPVA.Differentproportionalscaffoldsalsohaddifferentporousstructures.SEMprovedthatlargeamountofporousstructurecouldbeformed.Biocompatibilitytestshowedthattheextractedfluidofcompositematerialswasnontoxic,whichcouldpromotetheadhesionandproliferationofthefibroblast.Fibroblastcouldgrowonthescaffoldnormally.AporousscaffoldfortissueengineeringwithhighwatercontentcanbefabricatedbyPVA,GAGandCOL,whichhasexcellentcellbiocompatibility.Theporousstructureshowspotentialintissueengineeringandcellculture.更多还原

简介：客观:准备nanophase人工的骨头合成支架基于bionics，进满足在骨胶原和无机的部分以及黏合的代理人的不同分子的重量之间的比率怎么不同的理论，和探查影响支架的微观结构和性质。方法：在象内容比率和环境，然后nanophasecollagen/calcic的pH价值那样的某些反应条件下面在一起沉淀期间带钙的无机的分子到deposite到自我装配的骨胶原模板上盐被获得。以便改进机械性质，有性质和可控制的可被细菌破坏的活动被选择为黏合的代理人的稳定的平等者的poly乳的酸(PLLA)，然后瞄准人工的骨头支架(nanophasecollagen/calcicsalt/PLLA合成)被完成。在有isoproanol酒精的准备液体排水量方法以后，扫描电子显微镜(SEM)，传播电子显微镜(TEM)，fourier变换红外线的spectrometry(FTIR)，机械测试系统被执行到测试孔和密度，形态学，符合构造，作文和机械性质，respectively.Results:合成支架主要是的人工的综合骨头部分与B类型碳酸盐代替的骨胶原和钙磷酸盐创作了。crystallinity是低的，并且水晶尺寸到达类似于自然骨头的纳米。有效地使用的PLLA改进能在cancellous标准的地板水平到达的机械性质，并且与80%的高孔开发three-dimensional-porous结构。根据比较，在象黏合的PLLA的增加一样的骨胶原和钙的无机的盐之间的内容比率都在综合支架的微观结构上有效果，这能被看见，PLLA的另外分子的重量在机械properties.Conclusion上有效果:nanophase人工的骨头合成支架由biomimetic综合了进程是最答应为临床的申请的最佳的材料不管怎么从结构，作文和pr判定了之一

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简介：AIMTo估计角膜的矩阵作为脚手架过去常重建的非细胞组成的驼鸟张力亢进的盐溶液与一个消化方法相结合的损坏cornea.METHODSA习惯于decellularize驼鸟角膜。非细胞组成的角膜的矩阵的微观结构被传播电子显微镜学(TEM)和hematoxylin和曙红观察(H&；E)染色。机械性质被一个电流计和一台紧张机器检测。非细胞组成的角膜的矩阵也被移植进一个兔子角膜和cytokeratin3被用来检查有免疫力的phenotype.RESULTSThe微观结构和驼鸟角膜的机械性质很好在decellularization过程以后被保存。在vitro，甲基thiazolyltetrazolium结果表明非细胞组成的驼鸟角膜(AOC)的摘录没在增长上有禁止的效果角膜上皮或endothelial房间或在keratocytes上。兔子当角膜的混浊和接枝溶解发生在非细胞组成的猪的角膜(APC)时，薄片状的keratoplasty证明移植AOC进主人角膜透明、完全合并移植。重建的角膜的显型类似于有3首先在表面的上皮的房间layer.CONCLUSIONWe使用了的cytokeratin的高表情的一个正常兔子角膜重建的脚手架损坏了角膜的AOC。与猪的角膜相比，驼鸟角膜的解剖结构人的角膜接近那些。根据原则，那结构决定功能，薄片状的keratoplasty也证实了的异种皮移植AOC移植与APC接枝的相比产生了优异结果。

简介：Theobjectiveofthisstudywastoproducetheporouscollagen-chitosan/Glycosanminglycans(GAG)forcornealceil-seedimplantasathree-dimensionaltissueengineeringscaffoldtoimprovetheregenerationcorneas.Theeffectofvariouscontentofglycerolasformporousagenttocollagen-chitosan/GAGpreservedaporousdimensionalstructurewasinvestigated.Theheat-dryingwasusedtoprepareporouscollagen-chitosan/GAGscaffold.Theporemorphologyofcollagenchitosan/GAGwascontrolledbychangingtheconcentrationofglycerolsolutionanddryingmethods.TheporousstructuremorphologywasobservedbySEM.Thediameteroftheporesform10to50μm.Thehighlyporousscaffoldhadinterconnectingpores.Thecornealcellmorphologywasobservedunderthelightmicroscope.Theseresultssuggestthatcollagen-chitosan/GAGshowedthatcornealcellhaveformedconfluentlayersandresemblethesurfaceofnormalcornealcellsurface.

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简介：AIMTo与人的角膜的上皮的房间和成纤维细胞调查角膜的前面的薄片状的重建的可行性，并且在vitro.METHODSThe支架的一个非细胞组成的猪的角膜矩阵(APCM)从与0.5%钠dodecyl硫酸盐(SDS)被对待的新鲜猪的角膜被准备答案和角膜的房间的完全的移动被hematoxylin曙红证实(他)染色并且4′；，染色的6-diamidino-2-phenylindole(DAPI)。人的角膜的成纤维细胞和上皮的房间与沥滤是有教养的从APCM提取的液体，然后房间proliferative能力被MTT评估试金。构造人的角膜的前面的薄片状的代替，角膜的成纤维细胞被注入APCM并且为3d有教养，由culturing列在后面在基质构造的角膜的上皮的房间为另一10d出现。角膜的代替被分析由他染色，并且immunofluorescencestaining.RESULTSHistological检查显示在APCM由没有房间他染色，并且染色的DAPI没检测任何剩余DNA。从APCM的沥滤的液体几乎没在人的角膜的成纤维细胞和上皮的房间的增长能力上有小影响。在10d，一连续盖住APCM的表面的人的角膜的上皮的房间的3～5层被观察，并且注射角膜的成纤维细胞在支架以内散布了。构造的显型类似于正常人的角膜，与cytokeratin的高表示12在上皮的房间层和在stroma.CONCLUSIONCorneal的vimentin的高表示，前面的薄片状的代替能被与一个非细胞组成的猪的角膜矩阵栽培人的角膜的上皮的房间和成纤维细胞在vitro重建。这在vivo为进一步的移植打了基础。

简介：Theextracellularmatrix,whichincludescollagens,laminin,orfibronectin,playsanimportantroleinperipheralnerveregeneration.Recently,aSchwanncell-derivedextracellularmatrixwithclassicalbiomaterialwasusedtomimictheneuralniche.However,extensiveclinicaluseofSchwanncellsremainslimitedbecauseofthelimitedorigin,lossofanautologousnerve,andextendedinvitroculturetimes.Inthepresentstudy,humanumbilicalcord-derivedmesenchymalstemcells(hUCMSCs),whichareeasilyaccessibleandmoreproliferativethanSchwanncells,wereusedtoprepareanextracellularmatrix.WeidentifiedthemorphologyandfunctionofhUCMSCsandinvestigatedtheireffectonperipheralnerveregeneration.Comparedwithanon-coateddishtissueculture,thehUCMSC-derivedextracellularmatrixenhancedSchwanncellproliferation,upregulatedgeneandproteinexpressionlevelsofbrain-derivedneurotrophicfactor,glialcell-derivedneurotrophicfactor,andvascularendothelialgrowthfactorinSchwanncells,andenhancedneuriteoutgrowthfromdorsalrootganglionneurons.ThesefindingssuggestthatthehUCMSC-derivedextracellularmatrixpromotesperipheralnerverepairandcanbeusedasabasisfortherationaldesignofengineeredneuralniches.

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简介：Inanerawhencardiactransplantpatientsaresurvivingmoreimmediateissuesofrejectionandinfection,theprevalenceofmorechronicissuessuchascardiacallograftvasculopathy(CAV)isrising.Thiscasedescribesaman20yearsaftercardiactransplantwithhisfirstpresentationofCAV.Acutemyocardialinfarctionwasdiagnosedonthebasisofsymptomsandbiochemicalmarkersandoncoronaryangiography,andhewasfoundtohaveacriticalstenosisofthemidportionoftheleftanteriordescendingartery.Itwaselectedtotreatthispercutaneouslywithafullybioresorbablevascularscaffold(BVS)becauseofthediffusenatureofthediseaseprocess.Thiswassuccessfullyperformedwithopticalcoherencetomographyguidance.TheuseofBVSinCAVhasnotbeenwellstudied.ThisisoneoffewcasereportsdescribingtheuseofBVSinCAV.

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