简介：AbstractSepsis remains one of the leading causes of death globally, in spite of advanced developments in intensive care and better understandings of pathophysiology related to sepsis. There is no special treatment or drug available for sepsis, currently. Under normal circumstances, neutrophil is a major player in acute infection control. However, during sepsis, the migration abilities and antimicrobial functions of neutrophils are impaired, resulting in a dysregulated immune response. Recent studies have indeed demonstrated that blocking or reversing neutrophil migration and impaired antibacterial function can improve the outcomes in septic animal models. This article systemically synthesized information regarding related factors and signaling involved in the functions of neutrophils in sepsis. This review also discussed the possibility that neutrophils be used as a marker for specific diagnosis and/or prediction of the outcomes of sepsis.

简介：AbstractOur study aimed to determine the effect of the neutrophil-lymphocyte ratio on the prognosis of adult patients with acute stroke. We searched the Web of Science, PubMed, Embase, Cochrane Library, and China National Knowledge Infrastructure databases and selected all of the potentially eligible studies. From the included studies, we extracted characteristics such as the stroke type and acquisition time until routine blood collection and the odds ratios across studies. The 95% confidence intervals and odds ratios were pooled to calculate the effect size for the neutrophil-lymphocyte ratio in acute stroke patients. We defined poor function outcomes according to the modified Rankin Scale ≥ 3 or Glasgow Outcome Scale< 3.Thirteen studies with 4443 patients were included in our analysis, including 7 ischemic and 6 hemorrhagic stroke studies. The pooled odds ratios for poor functional outcome at 3 months with a higher neutrophil-lymphocyte ratio in acute ischemic and hemorrhagic patients were 1.689 (95% CI = 1.184-2.409, p < 0.001) and 1.125 (95% CI = 1.022-1.239, p < 0.001), respectively, and the overall pooled odds ratio for poor functional outcome following stroke was 1.257 (95% CI = 1.146-1.379, p < 0.001). At the same time, the overall combined odds ratio for death at 3 months was 1.632 (95% CI = 1.155-2.306, p < 0.001).The neutrophil-lymphocyte ratio, an easily calculated marker, plays a predictive role in the short-term outcomes of adult patients (mean age ≥ 50 years) following acute ischemic and hemorrhagic stroke.

简介：LFA-1andMac-1,twoβ2integrinmembersconstitutivelyexpressedonneutrophils,mediateleukocyterecruitmentcascadebybindingtothesameligandofICAM-1.TheslowrollingandfirmadhesionofleukocytesrelyonLFA-1whilethecellcrawlingisdependentonMac-1.Wehypothesizedthattheirdistinctroleswerelikelyattributedtothedifferencesinthebindingkineticsorinthediverseresponsesofoutside-inandinside-outsignaling.Inthisstudy,wecomparedtheICAM-1bindingfeaturesbetweensolubleormembrane-expressedLFA-1andMac-1withdifferentaffinityconformationsusingopticaltraptechnique.Ourdataindicatethattheaffinityup-regulationfromwidetype(WT)tohighaffinity(HA)isoff-ratedependentforLFA-1buton-ratedependentforMac-1.Thestructuralbasesofthisnewfindingwerefoundtobeconsistentwithourprevioussimulations.Theseresultsfurtheredourunderstandingontheirfunctiondifferencesundershearflow.

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简介：AbstractBackground:Although a variety of risk factors of pneumonia after clipping or coiling of the aneurysm (post-operative pneumonia [POP]) in patients with aneurysmal subarachnoid hemorrhage (aSAH) have been studied, the predictive model of POP after aSAH has still not been well established. Thus, the aim of this study was to assess the feasibility of using admission neutrophil to lymphocyte ratio (NLR) to predict the occurrence of POP in aSAH patients.Methods:We evaluated 711 aSAH patients who were enrolled in a prospective observational study and collected admission blood cell counts data. We analyzed available demographics and baseline variables for these patients and analyzed the correlation of these factors with POP using Cox regression. After screening out the prognosis-related factors, the predictive value of these factors for POP was further assessed.Results:POP occurred in 219 patients (30.4%) in this cohort. Patients with POP had significantly higher NLR than those without (14.11 ± 8.90 vs. 8.80 ± 5.82, P < 0.001). Multivariate analysis revealed that NLR remained a significant factor independently associated with POP following aSAH after adjusting for possible confounding factors, including the age, World Federation of Neurosurgical Societies (WFNS) grade, endovascular treatment, and ventilator use. And the predictive value of NLR was significantly increased after WFNS grade was combined with NLR (NLR vs. WFNS grade × NLR, P = 0.011).Conclusions:Regardless of good or poor WNFS grade, patients having NLR >10 had significantly worse POP survival rate than patients having NLR ≤10. NLR at admission might be helpful as a predictor of POP in aSAH patients.

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简介：AbstractObjective:The objective of this study was to investigate the expression levels of microRNA-141-5p(miRNA-141-5p), MAPK1 and neutrophil elastase in patients with and without preeclampsia (PE), and the relationship between miRNA-141-5p and MAPK1 with respect to the secretion of elastase by neutrophils in patients with PE.Methods:Thirty patients with PE and 30 healthy pregnant (HP) women were recruited from The Second Hospital of Shanxi Medical University, Taiyuan, China, between February 2017 and July 2018. Neutrophils were isolated from 8 mL peripheral blood samples and cultured. We recorded neutrophil count and morphology during culture. Apoptosis was detected by flow cytometry in different groups at 0, 24, and 48 h. The expression levels of elastase were detected in neutrophils by enzyme-linked immunosorbent assay, whereas the expression levels of miRNA-141-5p in peripheral blood neutrophils were detected by real-time polymerase chain reaction. We used TargetScanHuman Release 7.2 to analyze the target genes of miRNA-141-5p. The expression of MAPK1 in peripheral blood neutrophils was detected by western blotting. Data were analyzed by SPSS version 21.0 software, and comparisons between groups were carried out with the Student t test.Results:There was no significant difference between the PE and HP groups (P > 0.050) with regard to age or body mass index. The weight of newborns in the PE group (2846.00 ± 600.00 g) was significantly lower than that in the HP group (3055.00 ± 230.68 g). The number of neutrophilic granulocytes(NGs) in blood samples from the PE group was significantly higher than that in the HP group (P = 0.003). There was no significant difference between the groups with regard to morphology. Apoptosis in the PE group was delayed when compared with the HP group at different time points. The P value of apoptosis in the PE and HP groups were respectively 0.790, < 0.001 and 0.030 at 0 h, 24 h and 48 h. The expression levels of miRNA-141-5p in the PE group were significantly lower than those in the HP group (P < 0.050). The expression levels of MAPK1 in neutrophils from the PE group were significantly higher than those in the HP group (P < 0.050) by western blot. The expression levels of elastase in neutrophils from the PE group were significantly higher than those in the HP group (P < 0.050). Furthermore, the number of NGs in peripheral blood from the PE group was higher than that of the HP group; however, the levels of apoptosis were lower. The expression levels of miRNA-141-5p in NGs decreased, the expression of MAPK1 increased, and the secretion of neutrophil elastase in the NG medium increased in the PE group than those in the HP group.Conclusion:Collectively, our analysis suggested that miRNA-141-5p may be involved in the pathogenesis of PE by regulating the MAPK1 signaling pathway to activate neutrophils and increase the secretion of elastase.

简介：为neovascular在诊断和预后调查neutrophil-to-lymphocyte比率(NLR)和platelet-to-lymphocyte比率(PLR)的地方年龄相关的有斑点的退化(AMD).METHODSOne百个AMD病人和100健康控制在学习被包括。血样品从静脉的血被获得，它被用于平淡的分析，并且这些样品被使遭到完成血计数。NLR被定义为淋巴细胞的数字划分的嗜中性的计数，并且PLR被定义为lymphocytes.RESULTSNo的数字划分的血小板计数统计上重要的差别以人口统计的特征在考虑下面在二个组之间被观察(P>0.05)。在耐心的组的平均NLR被发现在健康控制组比那显著地高(P<0.05)。平均PLR作为与控制组相比在耐心的组是显著地更高的(P<0.05)。当最好改正的视觉尖酸(BCVA)增加了，NLR和PLR减少了(在49.8%和63.0%点的重要否定关联，分别地)而当中央有斑点的厚度(CMT)增加了，NLR和PLR增加了(在59.3%和70.0%点的重要积极关联，分别地).CONCLUSIONNLR和PLR层次作为与健康控制相比在neovascularAMD病人之中是更高的组。NLR和PLR层次被发现与BCVA并且直接相反地成正比与CMT成正比。

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简介：AbstractBackground:Follistatin-like 1 (FSTL1) plays both pro-inflammatory and anti-inflammatory roles in the inflammatory processes. We investigated whether serum FSTL1 could predict the current anti-neutrophil cytoplasmic antibody-associated vasculitis (AAV)-specific indices.Methods:We randomly selected 74 patients with AAV from a prospective and observational cohort of Korean patients with AAV. Clinical and laboratory data and AAV-specific indices were recorded. FSTL1 concentration was determined using the stored sera. The lowest tertile of the short-form 36-item health survey (SF-36) was defined as the current low SF-36. The cutoffs of serum FSTL1 for the current low SF-36 physical component summary (PCS) and SF-36 mental component summary (MCS) were extrapolated by the receiver operator characteristic curve.Results:The median age was 62.5 years (55.4% were women). Serum FSTL1 was significantly correlated with SF-36 PCS (r = -0.374), SF-36 MCS (r = -0.377), and C-reactive protein (CRP) (r = 0.307), but not with Birmingham vasculitis activity score (BVAS). In the multivariable linear regression analyses, BVAS, CRP, and serum FSTL1 were independently associated with the current SF-36 PCS (β = -0.255, β = -0.430, and β = -0.266, respectively) and the current SF-36 MCS (β = -0.234, β=-0.229, and β= -0.296, respectively). Patients with serum FSTL1 ≥779.8 pg/mL and those with serum FSTL1 ≥841.6 pg/mL exhibited a significantly higher risk of having the current low SF-36 PCS and SF-36 MCS than those without (relative risk 7.583 and 6.200, respectively).Conclusion:Serum FSTL1 could predict the current functional status in AAV patients.

简介：我们以前报导在C3H/HeN(C3H)老鼠的neutrophils的巨大的渗入不能高效地控制感染和力量贡献的Chlamydiamuridarum(厘米)到肺感染的这些老鼠的高危险性。为了推进，在chlamydial感染期间在C3H鼠标定义嗜中性的回答的性质，分别地，我们检验与neutrophils渗入和激活有关的粘附分子和CD11b的表达式后面的intranasal厘米感染。结果证明selectins(E-selectin，P-selectin和L-selectin)的表示，和在C3H老鼠的肺的细胞间的房间粘附molecule-1(ICAM-1)比在C57BL/6(B6)更显著地增加了老鼠，更抵抗的紧张。这些结果在C3H老鼠与巨大的neutrophils渗入相关很好。相反，外部血上的CD11b表示和在C3H鼠标的肺neutrophils在感染(白天14)的迟了的噬菌体期间与B6鼠标相比展出了重要减小。这些调查结果建议在C3H鼠标的粘附分子的高级表达式可以提高neutrophils招募到肺，但是neutrophils上的CD11b表示的衰落可以稀释嗜中性的功能。因此，neutrophils上的CD11b下面规定可以贡献C3H老鼠的失败控制chlamydial肺感染。

简介：嗜中性的渗入是含酒精的steatohepatitis的一个特点；然而，内在的机制仍然保持不清楚。我们以前报导synergistically喂的chronic-plus-binge乙醇导致neutrophils的肝的招募，它贡献肝损害。在这份报纸，我们在chronic-plus-binge乙醇喂导致调查了不变的自然漂亮T(iNKT)房间的角色肝的嗜中性的渗入和肝损害。野类型并且iNKT的二紧张房间缺乏的老鼠(CD1d缺乏并且Jα；18-deficient老鼠)受到喂的chronic-plus-binge乙醇。肝损害和发炎被检验。synergistically喂的Chronic-plus-binge乙醇增加了肝的iNKT房间的数字并且导致了他们的激活，与独自的长期的喂或饮酒作乐相比。iNKT房间缺乏的老鼠被保护免受chronic-plus-binge的伤害导致乙醇的肝的嗜中性的渗入和肝损害。而且，显著地喂几基因的肝的表示在野类型的老鼠与发炎和嗜中性的招募联系了的upregulated的chronic-plus-binge乙醇，而是这些基因的正式就职在iNKT被废除房间缺乏的老鼠。重要地，几cytokines和chemokines(例如，MIP-2，MIP-1，IL-4，IL-6和osteopontin)在嗜中性的渗入包含了在肝的NKT房间的upregulated从chronic-plus-binge被孤立与喂对的老鼠相比的喂乙醇的老鼠。最后，有堵住抗体的CD1d的处理，堵住iNKT房间激活，部分阻止了chronic-plus-binge导致乙醇的肝损害和发炎。喂的Chronic-plus-binge乙醇激活肝的iNKT房间，它在早含酒精的肝损害的发展起一个关键作用，部分地由释放招募的调停人neutrophils到肝，并且这样，iNKT房间为含酒精的肝的治疗代表一个潜在的治疗学的目标疾病。