简介:AbstractBackground:Cerebral cavernous malformations (CCMs), a major neurosurgical condition, characterized by abnormally dilated intracranial capillaries, result in increased susceptibility to stroke. KRIT1 (CCM1), MGC4607 (CCM2), and PDCD10 (CCM3) have been identified as causes of CCMs in which at least one of them is disrupted in most familial cases. Our goal is to identify potential biomarkers and genetic modifiers of CCMs, using a global comparative omics approach across several in vitro studies and multiple in vivo animal models. We hypothesize that through analysis of the CSC utilizing various omics, we can identify potential biomarkers and genetic modifiers, by systemically evaluating effectors and binding partners of the CSC as well as second layer interactors.Methods:We utilize a comparative omics approach analyzing multiple CCMs deficient animal models across nine independent studies at the genomic, transcriptomic, and proteomic levels to dissect alterations in various signaling cascades.Results:Our analysis revealed a large set of genes that were validated across multiple independent studies, suggesting an important role for these identified genes in CCM pathogenesis.Conclusion:This is currently one of the largest comparative omics analysis of CCM deficiencies across multiple models, allowing us to investigate global alterations among multiple signaling cascades involved in both angiogenic and non-angiogenic events and to also identify potential biomarker candidates of CCMs, which can be used for new therapeutic strategies.
简介:摘要目的采用流式细胞术SP细胞分选法分离并鉴定胰腺癌SP细胞符合胰腺癌干细胞特性。方法1)选用人胰腺癌细胞系BxPC-3、PANC-1、SW1990,采用流式细胞术SP分选法分离出SP细胞;2)采用平板克隆实验、细胞分化实验、裸鼠成瘤实验研究SP细胞亚群的基本生物学特性。结果1)人胰腺癌细胞系BxPC-3、PANC-1、SW1990均含有SP细胞亚群,比例(%)分别是0.58±0.02,8.74±0.33,3.92±0.16;2)胰腺癌细胞SP细胞亚群和non-SP细胞亚群克隆形成能力、增殖分化能力有差异(P<0.05),SP细胞具有更强的克隆形成能力和增殖分化能力;SP细胞的裸鼠成瘤能力至少是非non-SP的100倍。结论胰腺癌SP细胞具有胰腺癌干细胞特性,流式细胞术SP分选法是分离胰腺癌干细胞的可靠方法。
简介:MaterialFlowAnalysis(MFA)isacrucialinstrumentforsustainabledevelopmentandcreatingindustrialecologysystem.MFAstudiescouldbalanceandanalyzethesources,flows,andconsumesofspecificmaterialsorsubstances.TheresultsofMFAstudiescouldsupportthestrategiesordecisionsmakingforenergy,resource,andwastemanagement,especiallyachievingsustainableresourcemanagement.AtCSC,thedynamicsimulatingsoftware,STELLA,isusedtodevelopaMFAmodelforscenarioanalysis.CSCalsousesthefreewareSTAN2.0asatoolforvisualizingandsimulatingmaterialflowsandstocks.CasestudiesofgreenhousegasesMFAforintegratedsteelworksareconducted.Theresultsshowedthatthecarboncontentofhot-metalisanimportanthiddenflowforbalanceanalysis,andthedifferentGHGemissionscenariosandmitigationactionscenariosareassessed.Inaddition,theIron-makingGHGI/OMFAModel,basedonworldsteelGlobalSteelSectorApproach(GSSA),isdevelopedforcalculatingtheCO2andenergyintensityofcokemaking,sintering,andBFprocesses.ThisMFAmodelwasusedtoanalyzetheCO2reductionpotentialforiron-makingprocess.ThecasesconductedforMFAapplicationsatCSCweresuchasgreenhousegas,zinc,etc.Inthefuture,CSCisgoingtodevelopa'CSCEnvironmentManagementandDecisionSupportingSystem'whichcombineMFA,LCA(lifecycleassessment),andenvironmentriskassessment.Thissupportingsystemexpectstopromotingenergyefficiencyandbestresourceuse,supportingenvironmentpolicymaking,creatingenvironmentalinformationvalue,etc.
简介:<正>至2004年底,我国加入WTO已近3年,也意味着针对汽车产业的"入世"保护期也走到尽头。在这3年中。中国汽车发生了怎样的变化?恐怕最直观的,就是老百姓面对众多品牌和车型选择上的增强。几年前还在为"老三样"充斥大街小巷而满腹牢骚的中国车迷,如今已经彻底理解了"审美疲劳"的含意:2001年国内轿车企业只有14家(不含吉利、美日、悦达、英格尔、百利等),乘用车型不过36种;而随着加入WTO后各汽车跨国公司争先建厂,今天市场上主流的乘用车品牌据不完全统计已达103种,如果再算上每个车型下属的各种排量、配置的细分车型,总数不下四五百种。形形色色的各式车型不但令购车者眼花缭乱,连汽车业内人士对不少车型的价格配置往往也是一头雾水。