简介：垂体腺瘤是一种常见的颅内肿瘤，占所有颅内肿瘤的10％～15％。虽然大多数垂体腺瘤是良性肿瘤，但是它常常引起一些内分泌症状，如巨人症、闭经泌乳及Cushing综合征等；另外由于其在颅内的特殊位置，肿瘤的增大和侵袭往往引起头痛、视力下降及视野缺损等症状。虽然近年来垂体腺瘤的诊断和治疗已取得长足的进步，但是部分垂体腺瘤尤其是侵袭性垂体腺瘤对于神经外科医师而言，

简介：MicroRNAs(miRNAs)aredynamicallyregulatedduringneurodevelopment,yetfewreportshaveexaminedtheirroleinspinabifida.Inthisstudy,weusedanestablishedfetalratmodelofspinabifidainducedbyintragastricallyadministeringoliveoil-containingall-transretinoicacidtodamsonday10ofpregnancy.Damsthatreceivedintragastricadministrationofall-transretinoicacid-freeoliveoilservedascontrols.ThemiRNAexpressionprofileintheamnioticfluidofratsat20daysofpregnancywasanalyzedusinganmiRNAmicroarrayassay.Comparedwiththatincontrolfetuses,theexpressionofmiRNA-9,miRNA-124a,andmiRNA-138wassignificantlydecreased(>2-fold),whereastheexpressionofmiRNA-134wassignificantlyincreased(>4-fold)intheamnioticfluidofratswithfetusesmodelingspinabifida.Theseresultswerevalidatedusingreal-timequantitativereverse-transcriptionpolymerasechainreaction.HierarchicalclusteringanalysisofthemicroarraydatashowedthatthesedifferentiallyexpressedmiRNAscoulddistinguishfetusesmodelingspinabifidafromcontrolfetuses.OurbioinformaticsanalysissuggestedthatthesedifferentiallyexpressedmiRNAswereassociatedwithmanycytologicalpathways,includinganervoussystemdevelopmentsignalingpathway.ThesefindingsindicatethatfurtherstudiesarewarrantedexaminingtheroleofmiRNAsthroughtheirregulationofavarietyofcellfunctionalpathwaysinthepathogenesisofspinabifida.Suchstudiesmayprovidenoveltargetsfortheearlydiagnosisandtreatmentofspinabifida.

简介：突触是神经元之间或神经元与效应器细胞之间相互接触和传递信息的部位，是产生神经功能和维持神经活动的基础。突触可塑性是指神经系统在外界因素的作用下，多种机制介导的突触结构或功能适应性变化。突触可塑性作为神经可塑性的一种特殊形式，主要包括突触发育的可塑性、突触形态的可塑性和突触传递的可塑性。

简介：神经胶质瘤是最常见的颅内原发性肿瘤,且其发病率随年龄增大有增高的趋势[1-2],也几乎是死亡率最高的脑内肿瘤。microRNA-203（miRNA-203）是新近发现的与胶质瘤密切相关的一类表达于真核生物的非编码蛋白质的短链RNA,近年来的研究发现miRNA-203在胶质瘤中的异常表达与胶质瘤发病的关系密切。

简介：Inthepresentstudy,weconstructedalentivirus,FIV-CMV-GFP-miR-7-3,containingthemicroRNA-7-3geneandthegreenfluorescentproteingene,andusedittotransfecthumangliomaU251cells.Fluorescencemicroscopyshowedthat80%ofU251cellsexpressedgreenfluorescence.Real-timereversetranscriptionPCRshowedthatmicroRNA-7-3RNAexpressioninU251cellswassignificantlyincreased.ProliferationwasslowedintransfectedU251cells,andmostcellswereintheG1phaseofthecellcycle.Inaddition,theexpressionoftheserine/threonineproteinkinase2wasdecreased.ResultssuggestedthattransfectionwithalentiviruscarryingmicroRNA-7-3caneffectivelysuppressepidermalgrowthfactorreceptorpathwayactivityinU251cells,arrestcellcycletransitionfromG1phasetoSphaseandinhibitgliomacellgrowth.