简介：NocathiacinI,aglycosylatedthiopeptideantibiotic,displaysexcellentantibacterialactivitiesagainstmultidrugresistantbacterialpathogens.Previously,anovelnocathiacinIformulationforintravenousadministrationhasbeensuccessfullydevelopedanditsaqueoussolubilityisgreatlyenhancedforclinicalapplication.ThepurposeofthepresentstudywastoincreasethefermentationtiterofnocathiacinIandreduceoreliminateanalogousimpuritiesbyscreeningthemediumingredientsusingresponsesurfacemethodology.Afterasysmaticoptimization,awater-solublemediumcontainingquality-controllablecomponentswasdevelopedandvalidated,resultinginanincreaseintheproductionofnocathiacinIfrom150to405.8mg·L-1at150-Lscale.Meanwhile,theanalogousimpuritiesexistedinreportedprocessesweregreatlyreducedoreliminated.Usingoptimizedmediumforfermentation,nocathiacinIwithpharmaceuticallyacceptablequalitywaseasilyobtainedwitharecoveryof67%.Inconclusion,theresultsfromthepresentstudyofferapracticalandefficientfermentationprocessfortheproductionofnocathiacinIasatherapeuticagent.

简介：Cancerisamajorhealthconcernandleadingburdenoneconomyworldwide.Anincreasingeffortisdevotedtoisolationanddevelopmentofplant-deriveddietarycomponentsaseffectivechemo-preventiveproducts.Phytochemicalcompoundsfromnaturalresourcessuchasfruitsandvegetablesareresponsiblefordecreasingtheriskofcertaincancersamongtheconsumingpopulations.Apigenin,aflavonoidphytochemicalfoundinmanykindsoffruitsandvegetables,hasbeenshowntoexertsignificantbiologicaleffects,suchasanti-oxidant,anti-inflammatoryandmostparticularlyanti-neoplasticproperties.Thisreviewisintendedtosummarizethemostrecentadvancesintheanti-proliferativeandchemo-preventiveeffectsofapigeninindifferentcancermodels.Analysisofthedatafromthestudiedcancermodelshasrevealedthatapigeninexertsitsanti-proliferativeeffectsthroughmultipleandcomplexpathways.Thisguidedustodiscoversomecontroversialresultsabouttheexactroleofcertainmolecularpathwayssuchasautophagyintheanticancereffectsofapigenin.Further,therewerecumulativepositiveevidencessupportingtheinvolvementofcertainpathwayssuchasapoptosis,ROSandDNAdamageandrepair.Apigeninpossessesahighpotentialtobeusedasachemosensitizingagentthroughtheup-regulationofDR5pathway.Accordingtothesepreclinicalfindingswerecommendthatfurtherrobustunbiasedstudiesshouldconsiderthepossibleinteractionsbetweendifferentmolecularpathways.

简介：目的：建立用超高效液相色谱（UPLC）测定肝癌前病变模型大鼠组织中丹皮酚的分析方法,研究丹皮酚在大鼠体内的组织分布特征。方法：采用UPLC测定大鼠组织中丹皮酚的浓度。结果：丹皮酚在对照组大鼠各组织中的分布顺序依次为肾〉胃〉小肠〉脑〉肝〉脾〉心〉肺,在模型组大鼠各组织中的分布顺序依次为肝〉肾〉脾〉肠〉胃〉心〉脑〉肺,且其在模型组各组织的AUCall均大于在对照组各组织的AUCall。结论：相对于对照组,丹皮酚在模型动物组织内含量增高。