简介：Shigellaflexneriisaninfectiouspathogenthatcausesdysenterytohuman,whichremainsaseriousthreattopublichealth,particularlyindevelopingcountries.Inthisstudy,theglobalproteinexpressionpatternsofS.flexneriduringtransitionfromexponentialgrowthtostationaryphaseinvitrowereanalyzedbyusing2-DPAGEcombinedwithMALDI-TOFMS.Inatime-courseexperimentwithfivetimepoints,therelativeabundanceof49proteinspotsvariedsignificantly.In-terestingly,aputativeoutermembraneproteinYciD(OmpW)wasalmostnotdetectedintheexponentialgrowthphasebutbecameoneofthemostabundantproteinsinthewholestationary-phaseproteome.SomeproteinsregulatedbytheglobalregulatorFNRwerealsosignificantlyinduced(suchasAnsB,AspA,FrdAB,andKatG)orrepressed(suchasAceEF,OmpX,SodA,andSucAB)duringthegrowthphasetransition.Theseproteinsmaybethekeyeffectorsofthebacterialcellcycleorplayimportantrolesinthecellularmaintenanceandstressresponses.Ourexpressionprofiledataprovidevaluableinformationforthestudyofbacterialphysiologyandformthebasisforfutureproteomicanalysesofthispathogen.