摘要
Anagrusnilaparvataeisthedominanteggparasitoidofriceplanthoppersandplaysanimportantroleinbiologicalcontrol.Symbioticbacteriacansignificantlyinfluencethedevelopment,survival,reproductionandpopulationdifferentiationoftheirhosts.TostudytheinfluenceoftemperatureonsymbioticbacterialcompositioninthesuccessivegenerationsofA.nilaparvatae,A.nilaparvataewereraisedunderdifferentconstanttemperaturesof22°C,25°C,28°C,31°Cand34°C.Polymerasechainreaction-denaturinggradientgelelectrophoresiswasusedtoinvestigatethediversityofsymbioticbacteria.OurresultsrevealedthattheendophyticbacteriaofA.nilaparvataewerePantoeasp.,Pseudomonassp.andsomeunculturedbacteria.ThebacterialcommunitycompositioninA.nilaparvataesignificantlyvariedamongdifferenttemperaturesandgenerations,whichmightbepartiallycausedbytemperature,feedingbehaviorandthephysicalchangesofhosts.However,theanalysisofwspgeneshowedthattheWolbachiainA.nilaparvataebelongedtogroupA,sub-groupMorsandsub-groupDro.Sub-groupMorswasabsolutelydominant,andthisWolbachiacompositionremainedstableindifferenttemperaturesandgenerations,exceptforthe3rdgenerationunder34°Cduringwhichsub-groupDrobecamethedominantWolbachia.Theaboveresultssuggestthatthecontinuoushightemperatureof34°CcaninfluencetheWolbachiacommunitycompositioninA.nilaparvatae.
出版日期
2016年04月14日（中国期刊网平台首次上网日期，不代表论文的发表时间）
