EFFECT OF LIPOSOMAL TRANSFECTION OF HTERT ON APOPTOSIS OF NEWBORN RAT COCHLEAR BASILAR MEMBRANE CELLS-中国期刊网

摘要 ObjectiveTheaimofthisstudyistoinvestigatetheeffectoftransfectedhTERTgeneoncellapoptosisofnewbornratcochlearbasilarmembranecells(CBMCs).MethodsCBMCsisolatedfromnewbornratcochlearweretransfectedusingaplasmidcontaininghumantelomerasereverasetranscriptasegene(pCI-neo-hTERT),andwerescreenedusingG418toobtainstabletransfectedcelllines.Cellapoptosisratewasanalyzedbyflowcytometry.hTERTandapoptosisrelatedgenesexpressionweredetectedbyreversetranscriptasepolymerasechainreaction(RT-PCR).ResultshTERTgeneexpressionwasdetected72hoursaftergenetransfectionintransfectedcells.TheapoptoticrateoftransfectedCBMCssignificantlyreduced.Expressionofapoptosisrelatedgenescorrespondinglychanged.ConclusionTransfectionofhTERTgeneleadstoreducedapoptosisrateinnewbornratCBMCs.andlowerexpressionofapaf1,Caspase3andBCL2intransfectedcellsascomparedtothatofnormalCBMCs.

1TANG,Lanyan. Therapeutic Effect of Bone Marrow Mesenchymal Stem Cells on Rat Bladder Cancer.,2023-03.
2LiHL YeKH. Effect of heparin on apoptosis in human nasopharyngeal carcinoma CNE2 cells.细胞生物学,2001-04.
3Yunong Wu Jiajia Zhang Xiaomeng Song Jinhai Ye Yangyu Zhen. The effect of human semaphorin 3f gene transient transfection on the proliferation of tongue carcinoma cells.口腔医学,2008-06.
4ZHANGMIN;HONGQINGZHANG;等. Effect of Bcl—2 and caspase—3 on calcium distribution in apoptosis of HL—60 cells.细胞生物学,2000-03.
5Li Ding;Xiao-Mao Hu;Hong Wu;Ge-Xiu Liu;Yang-Jun Gao;Dong-Mei He;Yuan Zhang. Combined transfection of Bcl-2 siRNA and miR-15a oligonucleotides enhanced methotrexate-induced apoptosis in Raji cells.肿瘤,2013-01.
6Richard Salvi. lead neurotoxicity in rat cochlear organotypic cultures.耳鼻咽喉科,2011-02.
7Jintao Yu;Dalian Ding;Hong Sun;Richard Salvi;Jerome A.Roth. Trimethyltin-induced cochlear degeneration in rat.耳鼻咽喉科,2016-03.
8Rong-Hua Xu;Liang-Yan Zheng;Dong-Lei He;Jian Tong;Li-Ping Zheng;Wu-Ping Zheng;Jin Meng;Li-Ping Xia;Cong-Jun Wang;Ji-Lin Yi. Effect of fragile histidine triad gene transduction on proliferation and apoptosis of human hepatocellular carcinoma cells.消化系统,2008-12.
9Jing Tan;Qing Zeng;Xian-Zheng Jiang;Le-Ye He;Jin-Rong Wang;Kun Yao;Chang-Hui Wang. Apoptosis of bladder transitional cell carcinoma T24 cells induced by adenovirus-mediated inducible nitric oxide synthase gene transfection.肿瘤,2013-05.
10郑华川;杨雪飞;孙晋民;李晓晗;姜卫国;张荫昌;辛彦. EXPRESSION OF MATRIX METALLOPROTEINASE-7 AND FAS LIGAND：THEIR APOPTOSIS-INDUCING EFFECT ON GASTRIC CANCER CELLS.肿瘤,2003-03.

EFFECT OF LIPOSOMAL TRANSFECTION OF HTERT ON APOPTOSIS OF NEWBORN RAT COCHLEAR BASILAR MEMBRANE CELLS

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EFFECT OF LIPOSOMAL TRANSFECTION OF HTERT ON APOPTOSIS OF NEWBORN RAT COCHLEAR BASILAR MEMBRANE CELLS

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